VIRAL-RNA
Quantification and kinetics of viral RNA transcripts produced in Orthohantavirus infected cells
... single-stranded RNA consisting of the Small (S), Medium (M) and Large (L) RNA segments [8, ...the viral RNAs and protects it from degradation. All viral RNA segments are coated with the ...
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Molecular Bases of Viral RNA Targeting by Viral Small Interfering RNA-Programmed RISC
... specific RNA degradation mediated by viral siRNA-pro- grammed ...of RNA silencing in natural virus infection, a new experimental system was further ...PVX-Cym viral ge- nome was efficiently ...
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Nucleocapsid-Independent Specific Viral RNA Packaging via Viral Envelope Protein and Viral RNA Signal
... non-MHV RNA transcript in the absence of N ...enveloped RNA viruses studied thus far, N protein or capsid protein plays an essential role in viral RNA ...MHV RNA packaging is ...non-MHV ...
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Complementary Mutations in the N and L Proteins for Restoration of Viral RNA Synthesis
... the viral RNA in the core ...sequestered RNA. In the NLP, the sequestered RNA is inaccessible if the helix 5-loop gate is in ...the viral RNA. During the viral RNA ...
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CNOT4 Mediated Ubiquitination of Influenza A Virus Nucleoprotein Promotes Viral RNA Replication
... enhances viral NP ubiquitination, whereas the deubiquitinase USP11 reduces ...of viral RNP, thereby enhancing their function in viral RNA ...enhances viral NP ubiquitination, whereas ...
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Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA
... all RNA, presumably to free ribonucleotides with 5’-hy- droxyls and 3’-phosphates, but also on the additional step of boiling at 100 °C for 10 min after pH has been neutralized to between pH 7 and ...in ...
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Four Proteins Processed from the Replicase Gene Polyprotein of Mouse Hepatitis Virus Colocalize in the Cell Periphery and Adjacent to Sites of Virion Assembly
... in viral RNA synthesis (3, 12, 33) and since N is also a structural component of the virion (6, 9, 18, 19, 35, 39–41), we expected that N would be abundant both at sites of replication and at sites of ...
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Functional Consequences of RNA 5′-Terminal Deletions on Coxsackievirus B3 RNA Replication and Ribonucleoprotein Complex Formation
... an RNA second- ary structure required for viral RNA ...of viral RNA in vitro, which was detected only for the 7- and 21- nucleotide ...I RNA forms a ribonucleoprotein complex ...
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RNA Interference and its application in management of viral diseases in plants
... antisense RNA expressed in transgenic plants and more directly by reports of unexpected outcomes in experiments performed by plant scientists in the ...to viral diseases observed a similar unexpected ...to ...
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RNA-Based Immunity Terminates Viral Infection in Adult Drosophila in the Absence of Viral Suppression of RNA Interference: Characterization of Viral Small Interfering RNA Populations in Wild-Type and Mutant Flies
... of viral RNA genomes in fruit flies and mosquitoes induces the production of virus-derived small interfering RNAs (siRNAs) to specifically reduce virus accumulation by RNA interference ...the ...
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Identification of a Natural Viral RNA Motif That Optimizes Sensing of Viral RNA by RIG I
... well and grown overnight at 37°C. RNA FISH was performed according to published protocols, with minor modifications (48). At different times after transfection, the cells were washed with phosphate-buffered saline ...
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Strand-Specific RNA Synthesis Defects in a Poliovirus with a Mutation in Protein 3A
... positive-strand viral RNA synthesis in vitro, viral cDNAs containing wild-type or mutant 3A coding sequences were engineered into plasmids containing the cis- active hammerhead ribozyme coding ...
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Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA.
... and viral RNA replication in the HeLa S10 translation-replication ...poliovirus RNA replication without affecting the polymerase elongation reaction (32, ...of RNA replication by guani- dine ...
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Synthetic nucleotides and their biological applications
... Early experiments of Bausek and Merigan (1969) on the dsRNA induction of interferon produced evidence for a temperature dependent step in the induction of interferon, whereby an inducer was susceptible to action of RNase ...
342
IFNs and STATs in innate immunity to microorganisms
... inside cells, such as species of the genera Listeria, Mycobacterium, Salmonella, Chlamydia, and Leishmania (11, 19–21, 30–33). Treatment with recombinant IFN- γ or, conversely, abrogation of IFN- γ responsive- ness ...
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RNA viral diseases of finfish: A Review
... and RNA- dependent RNA polymerase, respectively (Ashraf et ...SVC viral genome consists of one molecule of non-infectious linear single standard RNA (Hill et ...
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Multiple Functions of Rice Dwarf Phytoreovirus Pns10 in Suppressing Systemic RNA Silencing
... Taken together, our results show that RDV Pns10 plays important roles in enhancing viral replication, systemic move- ment, and invasion of new tissues and especially the shoot apices. The siRNA binding activity is ...
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