virus assembly
Efficient Virus Assembly, but Not Infectivity, Determines the Magnitude of Hepatitis C Virus-Induced Interferon Alpha Responses of Plasmacytoid Dendritic Cells
... C virus (HCV), seven distinct genotypes of which are ...C virus (JFH1) did not induce interferon alpha (IFN- ␣ ), whereas the intragenotypic chimera Jc1 triggered massive IFN-␣ ...of virus particles ...
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Mutations in the Basic Region of the Mason-Pfizer Monkey Virus Nucleocapsid Protein Affect Reverse Transcription, Genomic RNA Packaging, and the Virus Assembly Site
... the assembly of intracytoplasmic ...the virus assembly site at the plasma membrane is dependent on Gag (for a review, see reference ...new assembly site at the plasma membrane, to which ...
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Architect of Virus Assembly: the Portal Protein Nucleates Procapsid Assembly in Bacteriophage P22
... of assembly plateaued (Fig. 1A and B). An aliquot of the assembly product obtained from each in vitro reaction was run on a native agarose gel, and the particles were visualized via transmission electron ...
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Sequence Requirements for Localization of Human Cytomegalovirus Tegument Protein pp28 to the Virus Assembly Compartment and for Assembly of Infectious Virus
... recombinant virus containing the pp28Mut50 mutant and other viral proteins including gB, gM/ gN, and pp150 in infected cells were similar to that of the wild-type parental virus (data not ...mutant ...
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Role of Murine Leukemia Virus Nucleocapsid Protein in Virus Assembly
... the virus life ...leukemia virus (MLV) particle assembly and ...of virus-producing cells, we observed that some NC mutants exhibit various stages of budding defects at the plasma membrane and ...
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Host cytoskeleton in respiratory syncytial virus assembly and budding
... Neurotropic viruses such as herpes simplex viruses (HSV) need to travel long distances within neurons. HSV takes control over the cytoskeleton early in infec- tion by remodelling the cortical actin cytoskeleton [62]. The ...
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Annexin 2: a Novel Human Immunodeficiency Virus Type 1 Gag Binding Protein Involved in Replication in Monocyte-Derived Macrophages
... HIV assembly and budding in MDM (35, 38). Significantly, virus replication was markedly de- creased in MDM in which Anx2 had been transiently knocked down by siRNA methodology, indicating a potential ...
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Role of Nonstructural Protein NS2A in Flavivirus Assembly
... the assembly and secretion of VLPs in packaging cells, repPAC  gal NS2A-I59N RNA-transfected cells were seeded into the wells of a 96-well plate and allowed to incubate for 9 ...the assembly and secretion ...
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Virus Particle Release from Glycosphingolipid-Enriched Microdomains Is Essential for Dendritic Cell-Mediated Capture and Transfer of HIV-1 and Henipavirus
... immunodeficiency virus type 1 (HIV-1) exploits dendritic cells (DCs) to promote its transmission to T ...on virus particles and CD169/Siglec-1 on ...the virus assembly and budding site ...
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Viral DNA Synthesis Defects in Assembly-Competent Rous Sarcoma Virus CA Mutants
... sarcoma virus (RSV) that were designed to test whether these different phenotypes might indicate distinct functional subdomains in the ...destroyed virus infectivity, although either of two lethal ...
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T Cell-Macrophage Fusion Triggers Multinucleated Giant Cell Formation for HIV-1 Spreading
... of virus assembly at the site of cell-to-cell contacts, leading to virus transfer and the accumulation of mature virus particles in cytoplasmic membrane compartments in macrophages after only ...
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Budding Capability of the Influenza Virus Neuraminidase Can Be Modulated by Tetherin
... influenza virus encodes the neuraminidase (NA) which is responsible for cleaving terminal sialic acid residues off gly- coconjugates on both the virus particle and the host cell, thereby facilitating ...
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Critical Role of the Human T-Cell Leukemia Virus Type 1 Capsid N-Terminal Domain for Gag-Gag Interactions and Virus Particle Assembly
... on virus particle ...particle assembly were characterized, and modeling implicates that these residues reside at the dimer interface ...particle assembly, a surprising and somewhat unexpected ...
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Distinct Intracellular Trafficking of Equine Infectious Anemia Virus and Human Immunodeficiency Virus Type 1 Gag during Viral Assembly and Budding Revealed by Bimolecular Fluorescence Complementation Assays
... anemia virus (EIAV), an ungulate lenti- virus, has been used to examine the mechanisms of animal lentivirus assembly and budding, and the results of these stud- ies have provided novel insights into ...
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Infectivity of Moloney Murine Leukemia Virus Defective in Late Assembly Events Is Restored by Late Assembly Domains of Other Retroviruses
... of virus assembly and virion budding (34, ...for virus budding (21, 22, ...viral assembly to an M-MuLV PPPY ...virion assembly. The RSV and M-MuLV L-domains restored the assembly ...
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Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity
... immature virus-like structures [7] also indicates that this motif does not form in an immature ...foamy virus, contain an N- terminal β-hairpin ...leukemia virus for example, a virus which ...
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Detection of intermediates and kinetic control during assembly of bacteriophage P22 procapsid
... . Virus assembly is a process in which well organized, usually highly symmetric (icosahedral or helical), viral capsids are built by association of the protein subunits 2 ...their assembly 3 . While ...
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Analysis of Human Immunodeficiency Virus Type 1 Gag Dimerization-Induced Assembly
... initiate assembly via a cross-link mechanism. Indeed, assembly initia- tion of CASP1Cys proteins but not CASP1 proteins could be accomplished with the cysteine-specific cross-linkers BMH and ...
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Subcellular distribution of viral structural proteins during simian virus 40 infection.
... The possible mechanisms can be studied by using simian virus 40 SV40-infected cells, since viral structural polypeptides are transported into the cell nucleus for assembly into virus By [r] ...
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In Vitro Assembly of PB2 with a PB1-PA Dimer Supports a New Model of Assembly of Influenza A Virus Polymerase Subunits into a Functional Trimeric Complex
... in-solution assembly (six experiments) and 5 to 20% for solid- phase assembly (four experiments) compared with the activity of coexpressed ...in-solution assembly experiments (results not ...vitro ...
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