virus isolation
Newcastle Disease Virus Isolation and Its Prevalence in Uganda Poultry Farms
... for virus shadding and antibody titers by use of the HA-HI tests followed by virus isolation from the tissues using 11-day-old chick embryonated eggs and chicken embryonated fibroblasts ...
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Comparison of monoclonal antibody based sandwich enzyme linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions
... Comparison of Monoclonal Antibody-Based Sandwich EnzymeLinked Immunosorbent Assay and Virus Isolation for Detection of Peste des Petits Ruminants Virus in Goat Tissues and Secretions JER[r] ...
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Excretion of bovine herpesvirus 1 in semen is detected much longer by PCR than by virus isolation
... of virus excretion varied with the tests used. On the basis of the virus isolation tests, virus excretion periods of several days or weeks and, subsequently, intermit- tent virus ...
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Comparison of Immunohistochemistry and Virus Isolation for Diagnosis of West Nile Virus
... live virus is not present in formalin- fixed ...that virus isolation still works on severely autolyzed tissue while immunohistochemistry results may be ...
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Economic comparison of enzyme immunoassay and virus isolation procedures for surveillance of arboviruses in mosquito populations
... However, the use of two different primary isolation cell culture assays per mosquito pool increased the estimated total cost for virus isolation and identification to $4.48 to $9.48 per [r] ...
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Comparison of Sensitivities of Virus Isolation, Antigen Detection, and Nucleic Acid Amplification for Detection of Equine Influenza Virus
... No virus was detected in the samples from horse 1 by use of the outer nucleoprotein ...used. Virus was detected in these samples and in the samples collected on days 6 and 7 with the primers from the matrix ...
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Development of a New Method for Diagnosis of Rubella Virus Infection by Reverse Transcription Loop Mediated Isothermal Amplification
... Virus isolation is traditionally performed for the laboratory confirmation of rubella virus infection, but the sensitivity of virus isolation is low because of poor conditions of sample ...
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Pathogenesis of experimental salmonid alphavirus infection in vivo: an ultrastructural insight
... of virus challenged ...for virus isolation ...viable virus replication in the heart or could be as a consequence of the limitations encountered during sample ...the virus ...
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One-Step RT-qPCR assay for detection and quantification of equine infectious anemia virus in-vitro
... of virus infection, it is useful to elucidate the pathogenesis and persistence of viruses, allowing prediction of disease progression, and evaluating the effects of antiviral therapy as well (Iwami et ...general, ...
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Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells
... For this reason, we evaluated different methods for virus isolation and infection of cells in culture and attempted to establish optimal conditions for virus recovery from the PMCs of al[r] ...
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Establishment and Characterization of a Madin Darby Canine Kidney Reporter Cell Line for Influenza A Virus Assays
... Viral isolation in cell cultures is handicapped by the relatively longer times required to ob- tain test results (5 to 7 days) and substantial requirements for specialized materials, equipment, and labor (42), al- ...
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PCR for detection of rubella virus RNA in clinical samples
... rubella virus RNA using primers from the E1 open reading frame was ...rubella virus) and specific; five wild-type rubella strains and four vaccine strains were detected, and no nonspecific amplification of ...
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Lagos Bat Virus in Kenya
... Bats were collected by hand nets or manually in the caves and human dwellings and mistnetted around roosts or in locations of nocturnal foraging. Both adult and subadult animals (based on body size) were randomly ...
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An integrated target sequence and signal amplification assay, reverse transcriptase PCR enzyme linked immunosorbent assay, to detect and characterize flaviviruses
... To evaluate the sensitivity and specificity of the RT-PCR-ELISA, we analyzed 91 serum specimens previously shown to contain DEN virus as demonstrated by virus isolation in Aedes albopict[r] ...
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Clinical Features and Virological Findings in Children With Primary Human Herpesvirus 7 Infection
... for virus isolation and antibody ...an isolation of HHV-7 and HHV-6 from peripheral blood MNCs and antibody responses to both viruses in the 15 patients are shown in Table ...by virus ...
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AMERICAN ACADEMY OF PEDIATRICS, INC. PROCEEDINGS AND REPORTS
... DISTRIBUTION OF PARALYTIC CASES WITH REFERENCE TO VACCINE LoT, VACCINATION STATUS, AND TYPE OF POLIOMYELITIS VIRUS. ( Based on virus isolation or serologic change)[r] ...
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Laboratory Diagnosis and Potential Application of Nucleic Acid Biosensor Approach for Early Detection of Dengue Virus Infections
... dengue virus, the genus of Flaviviridae virus ...dengue virus and patients just solely depend on the treatment of the dengue infection ...dengue virus is very desirable, especially in resource ...
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Isolation of Bovine Herpesvirus-1 (BoHV-1) From Latently Infected/Carrier Cattle in Ahvaz
... including virus isolation or those immunological assays which detect specific antibodies against the virus such as virus neutralization test (VNT), enzyme-linked immunosorbent assay (ELISA) ...
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First isolation and genetic characterization of pseudocowpox virus from cattle in Japan
... Previously, there has only been one report describing the PCR detection of PCPV DNA in Japan, in which PCPV DNA was detected from oral lesions in a calf in Iwate Pre- fecture, in the northern part of Japan, but ...
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SUB-ACUTE SCLEROSING PAN ENCEPHALITIS A REPORT OF SEVEN CASES
... The cases reported here show clinical features of S.S.P.E. consistent with those generally described inS.P.P.E. On an average 2 to 3 cases per year have been seen in the Civil Hospital,during the last 8-10 years which ...
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