western blot
Line blot and western blot immunoassays for diagnosis of Mediterranean spotted fever
... Finally, we also evaluated whether the Western blot and line blot immunoassays exhibited any species specificity for MSF infection by testing MSF sera against six different spotted fever[r] ...
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Cytomegalovirus Destruction of Focal Adhesions Revealed in a High-Throughput Western Blot Analysis of Cellular Protein Expression
... conventional Western blot assays (MOI ⫽ 5) and the Powerblot (MOI ⫽ 5), with conventional Western blotting data on the left and those from the Powerblot on the ...Conventional Western ...
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Development of a Western Blot Assay for Detection of Antibodies against Coronavirus Causing Severe Acute Respiratory Syndrome
... by Western blotting for comparison with IFA results to further validate the reliability of our Western blot ...20 Western blot-positive samples from SARS patients, 5 Western ...
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Western blot analysis of the human antibody response to Campylobacter jejuni cellular antigens during gastrointestinal infection
... Western blot analysis was used to identify antigenic components of Campylobacterjejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enter[r] ...
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LDBio Toxo II Immunoglobulin G Western Blot Confirmatory Test for Anti Toxoplasma Antibody Detection
... Tests commonly used for routine determination of anti-Toxoplasma gondii immunoglobulin G (IgG) antibodies show a high level of consistency. However, considerable variations between commercial screening tests are still ...
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Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)
... Please cite this article as: Cuttell, L., G´omez-Morales, M.A., Cookson, B., Adams, P.J., Reid, S.A., Vanderlinde, P.B., Jackson, L.A., Gray, C., Traub, R.J.,Evaluation of ELISA coupled with Western blot as ...
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Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot
... Background: Trichinellosis is a meat-borne zoonotic disease caused by parasites of the genus Trichinella. To date, 12 taxa have been described. The identification of Trichinella species is crucial in order to identify ...
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Western Blot Analysis of Sera from Leishmania major-Infected BALB/c and C57BL/6 Mice
... Western blot analysis of antibody-reactive leishmanial antigens. Discontinuous SDS–PAGE was performed on 4% stacking gels over 10% sepa- rating gels. Parasites lysates were boiled at 100°C for 5 min in 6X ...
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Multicenter evaluation of the novel ABN Western blot (immunoblot) system in comparison with an enzyme linked immunosorbent assay and a different Western blot
... With the DuPont Western blot as the reference assay, the overall sensitivity and specificity of the ABN WesPage were 100 and 99.1%, respectively, when indeterminate results were not take[r] ...
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Evaluation of a Commercial Echinococcus Western Blot Assay for Serological Follow-Up of Patients with Alveolar Echinococcosis
... commercialized Western blot assay has been eval- uated for the serological confirmatory testing of echinococcosis and differentiation between infections with ...Echinococcus Western Blot IgG ...
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Evaluation of two sets of immunohistochemical and Western blot confirmatory methods in the detection of typical and atypical BSE cases
... molecular features and deposition patterns in the brainstem, independently of the geographical origin of the BSE isolates detected [18-21] or breed or route of inoculation in cases of experimental transmission [22]. In ...
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Western Blot Analysis of Sera Reactive to Human Monocytic Ehrlichiosis and Human Granulocytic Ehrlichiosis Agents
... by Western blotting may preclude definitive deter- mination of the specific ehrlichial ...context, Western blotting and the use of recombinant major outer membrane proteins can be helpful in discriminating ...
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Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis
... ELISA techniques currently available are designed to detect polyclonal antibodies that target the whole virion. The use of nucleoprotein as the antigen for diagnosis and evaluation of vaccine immune responses is an ...
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Bicentric Evaluation of Six Anti-Toxoplasma Immunoglobulin G (IgG) Automated Immunoassays and Comparison to the Toxo II IgG Western Blot
... For all the automated techniques studied, the sensitivities and specificities obtained should be analyzed and interpreted in parallel with the quantitative results for the discordant and borderline sera (Table 3). These ...
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Frequency, Causes, and New Challenges of Indeterminate Results in Western Blot Confirmatory Testing for Antibodies to Human Immunodeficiency Virus
... poses, Western blotting or line immunoassays with separated viral proteins immobilized on membrane testing strips gener- ate specific information on the reactivities of antibodies to individual proteins ...result, ...
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<p>Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species</p>
... the Western blot and dot-blot ELISAs; the signals of the corresponding negative samples were visibly similar to those of the positive samples (Figures 1 and ...the Western blot and ...
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Comparison of the western blot assay with the neutralizing antibody and enzyme linked immunosorbent assays for measuring antibody to verocytotoxin 1
... A Western blot (immunoblot) assay (WBA) was developed to detect immunoglobulin G (IgG) antibodies against Escherichia coli Verocytotoxin 1 (VT1) by using a chemiluminescence detection ...
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Detection of Potentially Diagnostic Leishmania Antigens with Western Blot Analysis of Sera from Patients with Cutaneous and Visceral Leishmaniases
... Western blot (WB) technique is considered more sensitive and specific than IFA and ELISA, particularly in cases with low antibody titration, asymptomatic VL and CL (8, ...
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Evaluation of the Aspergillus Western Blot IgG Kit for Diagnosis of Chronic Aspergillosis
... (ii) Aspergillus Western blot IgG kit. Each serum was tested using the Asp-WB IgG kit (LDBio Diagnostics, Lyon, France) according to the man- ufacturer’s instructions. Briefly, 1.2 ml of sample buffer was ...
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Evaluation of Western Blot CagA Seropositivity in Helicobacter pylori-Seropositive and -Seronegative Subjects
... In conclusion, our data suggest that for a major proportion of H. pylori-seronegative subjects who are CagA seropositive, the reaction to CagA is a false-positive reaction. Our results also suggest that there is a ...
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