Western blot protein analysis
Western blot analysis of antibody response to pneumococcal protein antigens in a murine model of pneumonia.
... Blood samples to be analyzed were obtained by retro-orbital puncture (individual kinetics of antibody response) or heart puncture. After centrifugation, serum samples were stored at 2 80°C until use. Antipneumococcal ...
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Voltage profile generation for simultaneous multi protein detection in western blot analysis
... The protein transfer unit of this device consists of a common cathode and a multi-anode plate, which applies a non- uniform voltage ...the protein samples isolated from bone-forming osteoblast cells in the ...
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Targeting JNK-interacting protein 1(JIP1) sensitises osteosarcoma to doxorubicin
... Osteosarcoma (OS) is the most common primary malignant bone tumour in children and adolescents. Despite aggressive therapy, survival outcomes remain unsatisfactory, especially for patients with metastatic disease or ...
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Ethylnitrosourea-Induced Mutation in Mice Leads to the Expression of a Novel Protein in the Eye and to Dominant Cataracts
... a protein of 119 amino acids and a calculated molecular weight of 13 ...kD. Western blot analysis using polyclonal antibodies against ␥-crystallins or the novel Aey1-specific protein ...
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HCMV Promotes Breast Cancer Metastasis: Impacts of CMVIL-10 in the Tumor Microenvironment
... Initial analysis of optimizations of knockdown of mDax-1 protein in siRNA treated mESC was confirmed by western blot ...mDax-1 protein was analyzed using antibodies specific to ...
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Identification of the Gene Encoding a 38-Kilodalton Immunogenic and Protective Antigen of Streptococcus suis
... suis protein(s) that can serve as a vaccine candidate or a diagnostic reagent, we constructed and screened a gene library with a polyclonal antibody raised against the whole-cell protein of ...
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Prokaryotic expression, purification of a novel candidate tumor suppressor gene FUS1 and characterization of its polyclonal antibodies
... FUS1 protein, FUS1 cDNA from MRC-5 cells was amplified by RT-PCR and cloned into prokaryotic expression vector ...SDS–PAGE analysis revealed that the ac- cumulation of the recombinant protein FUS1 ...
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Recombinant neuraminidase pseudotyped baculovirus: a dual vector for delivery of Angiotensin II peptides and DNA vaccine
... For protein synthesis, Western blot analysis of the cell lysates obtained from transduced HEK293A cells revealed a 55 kDa recombinant protein specifically bound to both anti-NA ...
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Original Article Sp1 is over-expressed in nasopharyngeal cancer and is a poor prognostic indicator for patients receiving radiotherapy
... specificity protein 1 (Sp1) protein expression with clinicopathological significance and disease prognosis in NPC patients receiving ...by western blot and immunohistochemical analysis, ...
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High Level Expression of Recombinant Ribosomal Protein (L7/L12) from Brucella abortus and Its Reaction with Infected Human Sera
... ribosomal protein, were involved in the immune response developed in the ...L7/L12 protein among bacteria and even eukaryotes raised questions about the nature of the antigenic motif that was recognized by ...
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Targeted ablation of the cellular inhibitor of apoptosis 1 (cIAP1) attenuates denervation-induced skeletal muscle atrophy
... by Western blot analysis of pro- tein extracted from the heart and spleen ...4c). Western blot analysis of protein extracts from the gastrocnemius muscle of wild-type and ...
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SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation
... by Western blot (WB) analysis ...WB analysis between meta- static lymph nodes tissuess (ML) than the matched pri- mary lymph node ...WB analysis also showed that SIRT7 protein ...
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Original Article Z-VRPR-FMK can inhibit the growth and invasiveness of diffuse large B-cell lymphoma by depressing NF-κB activation and MMP expression induced by MALT1
... Abstract: This study aimed to investigate the therapeutic effect of the mucosa-associated lymphoid tissue lym- phoma translocation gene 1 (MALT1) on diffuse large B-cell lymphoma (DLBCL) and its underlying molecular ...
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Characterization of Neospora caninum Surface Protein NcSRS2 Based on Baculovirus Expression System and Its Application for Serodiagnosis of NeosporaInfection
... surface protein of ...401-amino-acid protein from au- thentic NcSRS2 ...the protein contained a 53-amino-acid signal ...truncated protein was secreted in the culture ...sequence ...
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The rat striatum responds to nigro-striatal degeneration via the increased expression of proteins associated with growth and regeneration of neuronal circuitry
... iTRAQ analysis of the striatal proteome at 3 days post lesioning suggests that the expression of cer- tain synaptic proteins ...GTPase-activating protein) may also be affected by this denervation (Table 1; ...
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Theophylline action on primary human bronchial epithelial cells under proinflammatory stimuli and steroidal drugs: a therapeutic rationale approach
... Notes: Theophylline is not present. Protein levels were determined by Western blot analysis. The gray level of every band was measured to check the difference in the protein expressions ...
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Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis
... recombinant protein yield is a determinative factor in stimulation of the mucosal immune ...The protein expression level obtained with a recombinant ...k1 protein yield produced by the devised ...
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Localization and phosphorylation of Plasmodium falciparum nicotinamide/nicotinate mononucleotide adenylyltransferase (PfNMNAT) in intraerythrocytic stages
... residues, protein immunoprecipitation was performed in the soluble extract of the parasite using both murine and chicken serum ...to Western blot analysis with anti- phosphorylated S, Y and T ...
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Notch1 signaling regulates the proliferation and self-renewal of human dental follicle cells by modulating the G1/S phase transition and telomerase activity
... Notch1 protein among the five different HDFC groups was determined using western blot ...The protein concentrations were determined using the Bradford assay (Bio- ...the protein bands ...
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Original Article Proteomic analysis of knock-down HLA-G in invasion of human trophoblast cell line JEG-3
... and protein expression levels of matrix metalloproteinase 2 and matrix metalloproteinase 9, and in the proteome assay, there were 3 identified proteins namely, prefoldin 1, eukaryotic translation elongation factor ...
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