Western Blotting
Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia
... Concluding remarks. Among the assays evaluated, the com- bined usage of ELISA and confirmatory Western blotting seems to be the most suitable approach for serodiagnosis of tularemia. Microagglutination has ...
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Estrogen receptors alpha and beta in rat placenta: detection by RT-PCR, real time PCR and Western blotting
... To study whether the changes in gene expression reflect changes in protein, the expression of ERα and ERβ pro- teins were studied. For ERα, two antibodies were used, one raised against the hinge region of the ERα (ERα-H) ...
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Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective
... by western blotting and antibody testing for capacity to immunoprecipitate PTMA from tissue and the recombinant PTMA, it was evident that AntiCT antibody successfully immunopreci- pitated PTMA from the ...
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Detection of Herpes Simplex Virus Type 2 Specific Immunoglobulin G Antibodies in African Sera by Using Recombinant gG2, Western Blotting, and gG2 Inhibition
... The detection and differentiation of type-specific antibodies to herpes simplex virus type 1 (HSV-1) and HSV-2 has been limited to reference laboratories due to the limited laboratory tools for HSV type-specific antibody ...
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In Search of Regulators of LeSPL CNR by South Western Blotting and Yeast One Hybrid Library Screening System
... Gene expression regulatory networks are comprised of cis- and trans- acting fac- tors, and differences in gene expression are attributable to genetic variation. Ep- igenetic change can also be considered as an important ...
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Characterization of Polyclonal Antibody Induced by Autoantibody TPO (Thyroidperoxidase) From Autoimmune Thyroid Disease (AITD) Serum with ELISA and Western Blotting
... In western blotting method, Autoantibody TPO as antigen react with autoantibody TPO monoclonal antibody, so that positive reaction of antigen-antibody is indicated by the appearance of purplish blue stain ...
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Original Article Western blotting was used to detect ZAP-70 molecule from γδΤ cells in peripheral blood
... Abstract: Objective: To detect the expression of signal transduction molecule ζ-chain associated protein 70 (ZAP- 70) in activated γδΤ cells from human peripheral blood. Methods: The peripheral blood mononuclear cells ...
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Serological Differentiation of Murine Typhus and Epidemic Typhus Using Cross-Adsorption and Western Blotting
... 12 Western blots were interpreted in a blinded fashion by four members of the laboratory, the workers were unable to determine if the Western blot reac- tions for sera from six patients (patients 3, 7, 8, ...
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Immune complex transfer enzyme immunoassay that is more sensitive and specific than western blotting for detection of antibody immunoglobulin G to human immunodeficiency virus type 1 in serum with recombinant pol and gag proteins as antigens.
... immunoassays, Western blotting (Ortho kit), the conventional ELISA with five recombinant proteins of HIV-1 as antigens (Abbott kit), and the gelatin particle agglutination test with a lysate of HIV-1 as the ...
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Western Blotting Handbook and Troubleshooting Guide
... a Western blotting procedure is to separate the macromolecules using gel ...the blotting membrane. Detailed procedures for detection of a Western blot vary ...
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Differentiation of Larva Migrans Caused by Baylisascaris procyonis and Toxocara Species by Western Blotting
... performed Western blots, wherein the ...migrans. Western blot results showed that ...ELISA, Western blotting could be an efficient tool for diagnosis of this infection in ...
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Identification of a calcitriol-regulated Sp-1 site in the promoter of human CD14 using a combined western blotting elect rophoresis mobility shift assay (WEMSA)
... It is difficult to calculate the precise cost savings afforded by WEMSA over EMSA. However, our best estimate would be around 30%. This is based on the cost of biotinylated oligonucleotides in EMSA as compared to ...
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Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with Western blotting.
... 1 Localization of Discontinuous Epitopes of Herpes Simplex Virus Glycoprotein D: Use of a Nondenaturing "Native" Gel System of Polyacrylamide Gel Electrophoresis Coupled with Western Blo[r] ...
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Qualitative and quantitative analyses of Epstein-Barr virus early antigen diffuse component by western blotting enzyme-linked immunosorbent assay with a monoclonal antibody.
... 793 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We report the use of monoclonal antibody against the early antigen diffuse component anti-EA-D of Epstein-Barr virus[r] ...
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Western Blotting. Prepare samples:
... -block in 5% skim milk in TBST for 1 hour at room temperature (or in block desired for best results with your antibody). -incubate in plastic seal-a-meal bags in primary antibody at des[r] ...
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Immunoenzyme Western blotting analysis of antibody specificity in Aleutian disease of mink, a parvovirus infection.
... The sera of 27 pastel non-Aleutian and 15 sapphire Aleutian mink infected for various times with ADV Pullman strain, a low-virulence strain, were tested by blotting analysis, and represe[r] ...
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Molecular Markers in Acute and Chronic Phases of Human Toxoplasmosis: Determination of Immunoglobulin G Avidity by Western Blotting
... We characterized antigenic markers recognized by human serum samples from patients presenting with acute and chronic toxoplasmosis by the determination of immunoglobulin G (IgG) antibody avidity by a Western blot ...
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An overview of technical considerations for Western blotting applications to physiological research
... The general principles of detection for WB are the same as for other antibody-based assays, such as the enzyme-linked immunosorbent assay (ELISA) (Vol- ler et al., 1978). In general terms, the 2 ° Ab is conju- gated with ...
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Analysis of western blotting (immunoblotting) technique in diagnosis of congenital syphilis
... Lane a, sera from infants with symptomatic congenital syphilis; lanes b and c, sera from infants with symptomatic congenital syphilis before lane b and after lane c IgM fractionation; la[r] ...
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Western and Dot Blotting Analyses of Ehrlichia chaffeensis Indirect Fluorescent Antibody Assay Positive and Negative Human Sera by Using Native and Recombinant E chaffeensis and E canis Antigens
... Recently, human infections with an E. ewingii-like agent have been documented (2). Like those of E. ewingii-infected dogs (23), these patients’ sera are IFA and Western immuno- blot positive for both E. ...
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