whole cell lysate.
A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts
... experiment. Whole-cell lysate can be used as a source of candidate substrates, allowing screens to be performed on samples of specific relevance to the area of interest instead of on protein or ...
12
In vitro cytokine profiles and viability of different human cells treated with whole cell lysate of Mycobacterium avium subsp. paratuberculosis
... Mycobacterium avium subsp. paratuberculosis (MAP) is a zoonotic pathogen, a very slow growing bacterium which is difficult to isolate and passage in conventional laboratory culture. Although its association with Johne ’ ...
6
Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein Antigen (r56) of Orientia tsutsugamushi and Its Use in Enzyme-Linked Immunosorbent Assays
... purified whole-cell lysate of the Karp strain of ...Karp whole-cell lysate antigens correlated well (r 5 ...rickettsia-derived, whole-cell antigen currently used in ...
8
Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureus: implications in the pathogenesis of septic arthritis and other soft tissue infections
... cytokine/chemokine induced by S. aureus is not known at present. Previous studies by Wang and colleagues [31] have shown that inhibitors of p38 MAPK (SB202190) and ERK1/2 (PD98059) and inhibitors of Src Tyrosine kinase ...
14
Serological Response to Pasteurella multocida NanH Sialidase in Persistently Colonized Rabbits
... ELISA. Secondary antibody conjugates were acquired from Accurate Anti- body (Westbury, N.Y.). A whole-cell-lysate ELISA was performed as described by Kawamoto et al. (29, 30) (the Kawamoto ELISA) in ...
10
Myristylated polyomavirus VP2: role in the life cycle of the virus.
... Since the viruses and viral products analyzed resulted from multiple rounds of infection, that is, over a period of 14 days in the case of the whole-cell lysate experiments viruses harve[r] ...
7
B-Cell-Specific Peptides of Leptospira interrogans LigA for Diagnosis of Patients with Acute Leptospirosis
... The whole-cell lysate-based ELISA offers reasonable sensitivity and the possibility of handling many samples at one time; the major drawback of this test system is the need for the maintenance of ...
6
Inducible expression of catalytically active type 1 serine/threonine protein phosphatase in a human carcinoma cell line
... of whole cell lysates using antibody to PP1α revealed diminution of the endogenous PP1α signal after 4 hr of induction, while the 6His-HA-PP1α signal increased (Figure ...the whole-cell ...
12
In vitro synthesis of 9,10-dihydroxyhexadecanoic acid using recombinant Escherichia coli
... Hydroxy fatty acids are important compounds due to their wide variety of high end applications. The produc- tion of vicinal diols of fatty acids has not been reported in the literature. The current study is the first ...
10
Prion subcellular fractionation reveals infectivity spectrum, with a high titre-low PrPreslevel disparity
... MoRK13 whole cell lysate showed no neuropathological abnormalities (Additional file 5: Figure ...MoRK13-inf whole cell lysate and fraction #10 inocula, with the former having ...
17
5364.pdf
... There are several advantages of using protectides over other bulky groups to block the degradation entrance. First, since solid phase synthesis of peptides has been well established for many years, protectides are easily ...
253
Whole gel processing procedure for GeLC-MS/MS based proteomics
... carcinoma sample collected from the tissue archive of the department of pathology at the VU University medical center (VUmc), Amsterdam, the Netherlands was used in compliance with the institution’s ethical regulations. ...
9
Optimizing parameters for clinical scale production of high IL 12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate
... of cell factory to use. Cell factories are especially suitable for clinical-scale production of DCs as they could be easily adapted in a GMP closed-culture system to avoid con- tamination, and be stacked to ...
16
The mechanism of action of a single dose of methylprednisolone on acute inflammation in vivo
... supernatant beta glucuronidase could be accounted for by cell death and lysis, as estimated from measurements of soluble DNA. Only 15-20% of the LDH activity could be accounted for on the basis of cell ...
12
The Ability of Lysate PRF Induces Proliferation of Fibroblast Cells in Endodontic Regenerative Therapy
... Platelet rich fibrin (PRF) is a blood-processed product with one centrifugation step and no additional anticoagulants and bovine serum [4]. Lysate-PRF is part of a PRF containing cytokines, structured ...
6
Gingipain-dependent degradation of mammalian target of rapamycin pathway proteins by the periodontal pathogen Porphyromonas gingivalis during invasion
... virulence factors which contribute to tissue destruction and host invasion (Holt and Ebersole 2005, Tanner and Izard 2006, Sharma 2010, Stafford et al., 2012). Examples of such virulence factors include ...
23
Plasmid Vectors for Proteomic Analyses in Giardia: Purification of Virulence Factors and Analysis of the Proteasome
... Glutathione beads that had been incubated with the cleared lysate and extensively washed showed the presence of a protein correlating well with the expected size of the SBP-GST-ADI fusion (96.7 kDa) and smaller ...
10
Whole-cell biocatalysts by design
... 40]. Whole-cell biocatalysts were developed for Neu5Ac production by assembling a heterologous biosynthetic pathway in ...as whole-cell biocata- lyst ...
12
Epithelial cell α3β1 integrin links β catenin and Smad signaling to promote myofibroblast formation and pulmonary fibrosis
... Preserved acute lung injury response in FASC mice. (A and B) Five days after intratracheal bleomycin injury, littermate control (A) and FASC (B) lung sections (original magnification, × 20) were stained with H&E and ...
13
A thymidylate synthase ternary complex-specific antibody, FTS, permits functional monitoring of fluoropyrimidines dosing
... A new rat monoclonal antibody, FTS, recognizes TS-F in 5FU-treated cells and tissues of human and mice. Using FTS in immunoblot assays, we observed dose- dependent responses of 5FU in cell lines and tissues. A ...
8