whole-cell patch-clamp recording
Acid sensing ion channels: potential therapeutic targets for neurologic diseases
... 1). Whole-cell patch clamp recording studies show that an increase in intracellular Ca 2+ due to cerebral ischemia may be attenuated by the nonspecific Na + channel inhibitor amiloride ...
8
Characterization of intracortical synaptic connections in the mouse anterior cingulate cortex using dual patch clamp recording
... The anterior cingulate cortex (ACC) is involved in sensory, cognitive, and executive functions. Studies of synaptic transmission and plasticity in the ACC provide an understanding of basic cellular and molecular ...
12
In vivo whole-cell recording with high success rate in anaesthetized and awake mammalian brains
... mechanisms, whole-cell patch-clamp recording has become feasible for in vivo preparations including both anaesthetized and awake mammalian ...vitro whole-cell ...
14
High glutamate permeability and distal localization of Best1 channel in CA1 hippocampal astrocyte
... dual patch-clamp recording onto CA1 astrocytes to observe the actual membrane voltage during the voltage clamp ...two recording electrodes were sealed consecutively to a single ...
9
Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings
... potentials. Whole-cell record- ing pipettes had a resistance of between 4 to 6 MΩ and pipettes for cell-attached recordings 8–10 ...the cell-attached measurement of ensem- ble ...
9
Cloning, expression and identification of KTX-Sp4, a selective Kv1.3 peptidic blocker from Scorpiops pococki
... Whole-cell patch-clamp recordings were performed using an EPC 9 amplifier (HEKA Elektronik, Lambre- cht/Pfalz, Germany) at room temperature (22–24 ...for recording Kv cur- rents on DRG ...
8
Expression of functional inhibitory neurotransmitter transporters GlyT1, GAT-1, and GAT-3 by astrocytes of inferior colliculus and hippocampus
... Whole-cell patch-clamp experiments were done as de- scribed before ...the recording chamber was placed at an upright microscope equipped with infrared dif- ferential interference ...
16
GABA-mediated inhibitory postsynaptic currents recorded in rat hippocampal slices using intracellular and patch clamp recording techniques
... a cell to which it is synaptically ...the whole-cell configuration in a motoneurone, stimuli were applied to neighbouring cells until a synaptically connected interneurone was ...large patch ...
118
PubMedCentral-PMC5134327.pdf
... Multi-modal recording describes the simultaneous collection of information across distinct ...simultaneous patch clamp measurements with fast- scan cyclic voltammetry (FSCV) using optically isolated ...
20
Histamine induced modulation of olfactory receptor neurones in two species of lobster, Panulirus argus and Homarus americanus
... Whole-cell voltage-clamp recording from the isolated soma of a lobster olfactory receptor cell membrane potential held at —25 mV, normal patch solution: Eci= — 66mV.. Pressure ejection o[r] ...
14
GAL-021 and GAL-160 are Efficacious in Rat Models of Obstructive and Central Sleep Apnea and Inhibit BKCa in Isolated Rat Carotid Body Glomus Cells
... ml/min) recording chamber mounted on the stage of an Olympus CK40 inverted ...1°C). Whole cell patch clamp recordings were then obtained from type I cells in voltage clamp ...
13
The Effects of Zinc and Other Divalent Cations on M Current in Ventral Tegmental Area Dopamine Neurons
... Electrophysiological measurements were made with an Axopatch-1B patch-clamp amplifier (Axon Instruments, Union City, CA, USA). Microelectrodes were fabricated on a P-97 puller (Sutter Instrument Company, ...
14
A patch-clamp study of native and recombinant glutamate receptors
... the patch shown in Fig ...other patch gave an open time distribution with two exponential components: ...The patch illustrated in Fig ...during patch-clamp ...
159
Intracellular ATP can regulate afferent arteriolar tone via ATP sensitive K+ channels in the rabbit
... Taken together with the patch clamp studies, these data suggest that activation of KATP channels results in cell hyperpolarization and vasodilation, and permit the conclusion that ATP-se[r] ...
9
Schieder, Michael (2011): Funktionelle Charakterisierung des Ionenkanals TPCN2. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... achieve whole lysosome ...EPC-10 patch clamp amplifier (HEKA, Lam- brecht, Germany) and PatchMaster acquisition software ...for recording conditions of chloride currents, and ...
80
NOISE PERFORMANCE OF A PATCH VOLTAGE CLAMP AND A BILAYER VOLTAGE CLAMP
... frequency range of interest for patch clamping. In general distributed RC noise depends on the geometry of the pipette, the thickness and dielectric constant of the pipette wall, the thickness of the elastomer ...
20
Functional up-regulation of Nav1.8 sodium channel in Aβ afferent fibers subjected to chronic peripheral inflammation
... channel blocker or vehicle (DMSO 6%), for a total of three injections per rat [27]. Ambroxol was delivered be- tween the lumbar vertebrae L5 and L6 in a final volume of 25 μl to lightly anesthetized animals with a ...
17
Spontaneously oscillating K+ channel activity in transformed Madin Darby canine kidney cells
... configuration, we found that channel activity was oscillating. The frequency of channel oscillations is 1.1 +/- 0.1 min-1. At the peak of oscillatory channel activity, single-channel current was -2.7 +/- 0.05 pA, and in ...
7
Automatic tracking of cells for video microscopy in patch clamp experiments
... Patch-clamp experiments in cultures or brain slices require a careful assessment of tar- geted cells before placing the patch electrode [1]. Consequently, the microscope stage is constantly moved in ...
14
Dorsal unpaired median neurones in the insect central nervous system: towards a better understanding of the ionic mechanisms underlying spontaneous electrical activity
... activates at depolarizing potentials more positive than −70 mV and exhibits rapid time-dependent activation and inactivation. Half-maximal steady-state inactivation occurs at approximately −65 mV and, unlike the ...
16