Whole-cell patch-clamp: The Arcuate Nucleus
Glucose sensing in the hypothalamic arcuate nucleus: electrophysiological and mathematical studies
... 3.1 Electrophysiology - Whole-cell patch-clamp: The arcuate nucleus 3.1.1 Experimental groups and animal care Male Wistar rats, aged 5 to 8 weeks old, were used throughout this ...
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Properties of the Nucleo-Olivary Pathway: An In Vivo Whole-Cell Patch Clamp Study
... the cell to generate the rebound de- ...single cell level, are proportional to the duration of the GABAergic inhibition: short IPSPs are followed by a short decay in phase-match, whereas long IPSP is ...
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Whole-Cell Patch Clamp Investigations on Rapid Synaptic Scaling of Mouse CA1 Pyramidal Neurons under Optogenetic Stimulation
... One obvious way to resolve GABAergic signals would be to decrease the chloride content such that entering WC configuration actually dilutes the available Cl- pool, driving E Cl - > –70 mV. This would have the added ...
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Planar Patch Clamp: Advances in Electrophysiology
... the cell is positioned auto- matically by application of suction ...a cell has been sealed to the chip and whether the parameters correspond to the cell-attached or the whole-cell ...
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Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings
... as whole- cell patch-clamp recordings do not accurately describe the macro- scopic properties of voltage-activated channels 7–9 ...using cell-attached and cell- free ...
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Antagonistic modulation of NPY/AgRP and POMC neurons in the arcuate nucleus by noradrenalin
... the arcuate nucleus of the hypothalamus (ARH) satiety signaling (anorexigenic) pro- opiomelanocortin (POMC)-expressing and hunger signaling (orexigenic) agouti-related peptide (AgRP)-expressing neurons are ...
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Remodeling of the arcuate nucleus energy balance circuit is inhibited in obese mice
... a whole, we detected a considerable degree of apoptosis in chow-fed mice at 16 weeks of age, supporting the view of neuronal ...of cell death within older neurons may be decreased to compensate for the ...
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A patch-clamp study of native and recombinant glutamate receptors
... from whole-cell experiments, that kainate failed to desensitise AMPA ...However, whole-cell responses afford a limited resolution and AMPA receptors were found to desensitise (albeit slightly) ...
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Automatic tracking of cells for video microscopy in patch clamp experiments
... in patch-clamp experiments are very thick ( > 200 μ m), therefore, the image sensor register a mixture of focused cells with blurred structures from defocused ...a whole new ...
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Dual patch voltage clamp study of low membrane resistance astrocytes in situ
... Abstract Whole-cell patch clamp recording has been successfully used in identifying the voltage-dependent gating and conductance properties of ion channels in a variety of ...dual patch ...
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Hardware Manual Version 2.5 EPC 10 USB. Patch Clamp Amplifier
... 7.2 Capacitance Compensation The EPC 10 USB provides automatic procedures for both fast and slow capacitance subtraction. In both cases, the ongoing pulse protocols are suspended and short trains of square-wave pulses ...
The vertebrate neuronal gamma aminobutyric acid (GABAa) receptor and its modulation: a patch clamp study
... FOR PATCH-CLAMP RECORDING The microscope, manipulators and aspirator flasks were positioned on a steel baseplate, and enclosed in a copper Faraday ...the patch amplifier was employed as the canmon ...
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Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
... automated patch-clamp system, the following procedures were performed ...384-well patch-clamp chip, and entrapped in the holes of the wells by an automatic- ally applied ...standard ...
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NOISE PERFORMANCE OF A PATCH VOLTAGE CLAMP AND A BILAYER VOLTAGE CLAMP
... • Seal Noise: Although the precise physical nature of the so-called “gigaseal” is not completely understood, its importance to high resolution patch clamping in undeniable. The seal resistance is in parallel with ...
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Glucokinase activity in the arcuate nucleus regulates glucose intake
... that arcuate nucleus glucokinase regulates the set point for glucose and that altering arcuate nucleus glucok- inase activity alters the set point for glucose consumption ...the arcuate ...
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Metabolic and serotonergic modulation of hypothalamic arcuate nucleus neurones in vitro
... Bath application of 5-HT induced a membrane depolarisation in a sub-population of ARC neurones (30%), a response that persisted in the presence of TTX indicating a direct eftect.. 5-[r] ...
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Ultrastructural response of arcuate nucleus neurons to fasting in aged rats
... The arcuate nucleus of the hypothalamus (ARH) is involved in the control of energy homeostasis. Leptin — an adipocyte derived hormone — is known to act on the hypothalamic nuclei and thus to control body ...
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The effect of fasting on the ultrastructure of the hypothalamic arcuate nucleus in young rats
... 4A, C, 5A, 6A) among other ultrastructural chang- es. They were formed by two cisternae of the RER attached closely to each other. Their outer mem- branes were irregularly studded with ribosomes. The narrow cleft between ...
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Mapping neuronal inputs to Kiss1 neurons in the arcuate nucleus of the mouse.
... into arcuate (ARC) Kiss1 ...to arcuate Kiss1 neurons includ- ing proopiomelanocortin neurons in the ARC itself, kisspeptin neurons in the anteroventral periventricular nucleus, vasopressin neurons in ...
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Automated Patch Clamp System Software Release Notes
... There is now a warning message at the beginning of the experiment reminding the user to manually place a cellboat with cells on the process deck. Keep in mind that if the cell boat is present and has not been ...
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