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whole genome amplification

Forensic Applications of Whole Genome Amplification

Forensic Applications of Whole Genome Amplification

... A review of the literature was carried out in order to assess the latest research and to identify a potentially fit for purpose whole genome amplification technique. There are three potential ...

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Forensic Applications of Whole Genome Amplification

Forensic Applications of Whole Genome Amplification

... A review of the literature relating to the forensic applications of whole genome amplification (WGA) has been carried out. DNA profiling techniques are undoubtedly a valuable forensic investigations ...

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Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification

Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification

... selective whole genome amplification (sWGA) strategy, originally described by Leichty and Brisson [8], uses computationally selected short oligonucleotide probes of 8–12 mers as primers that ...

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Whole genome amplification and its impact on CGH array profiles

Whole genome amplification and its impact on CGH array profiles

... two whole genome amplification methods for STR genotyping of LCN and degraded DNA ...of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage ...panel. ...

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Whole genome amplification and exome sequencing of archived schistosome miracidia

Whole genome amplification and exome sequencing of archived schistosome miracidia

... for whole genome amplification of Schistosoma mansoni and Schistosome haematobium miracidia from these cards, as well as real time PCR quantifi- cation of amplified schistosome ...using ...

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A systematic evaluation of whole genome amplification of bisulfite-modified DNA

A systematic evaluation of whole genome amplification of bisulfite-modified DNA

... Whole genome amplification of bisulfite-modified DNA We performed multiple displacement amplification (MDA)-based WGA by phi29 DNA polymerase using sodium bisulfite-modified DNA as the ...

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Whole genome amplification of degraded and nondegraded DNA for forensic purposes

Whole genome amplification of degraded and nondegraded DNA for forensic purposes

... Received: 31 May 2012 / Accepted: 14 August 2012 / Published online: 1 September 2012 # The Author(s) 2012. This article is published with open access at Springerlink.com Abstract Degraded DNA is often analyzed in ...

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DNA profiling of sperm cells by using micromanipulation and whole genome amplification.

DNA profiling of sperm cells by using micromanipulation and whole genome amplification.

... The aim of this study was to develop a method that enables DNA profiling of up to a single sperm cell. Spermatozoa were isolated through micromanipulation. Spermatozoa were lysed and their DNA was pre-amplified by ...

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Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance

Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance

... human genome sequence, SNP identification efforts and the development of high-throughput genotyping platforms ...performing whole genome association studies ...assays. Whole genome ...

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Analysis of Archived Residual Newborn Screening Blood Spots After Whole Genome Amplification

Analysis of Archived Residual Newborn Screening Blood Spots After Whole Genome Amplification

... using whole exome sequencing [4]. Here we assess the effects of whole genome amplification on our ability to identify real variation in exome sequence data as an applica- tion for samples with ...

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Efficiency of whole genome amplification of single circulating tumor cells enriched by CellSearch and sorted by FACS

Efficiency of whole genome amplification of single circulating tumor cells enriched by CellSearch and sorted by FACS

... DNA amplification SKBR-3 sorted into a 384-well ...4°C. Whole genome amplification mix (5 μ l) was added and incubated for 250 minutes at 30°C on a BioRad CFX 384 quantitative PCR machine ...

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Short tandem repeat analysis after whole genome amplification of single B-lymphoblastoid cells

Short tandem repeat analysis after whole genome amplification of single B-lymphoblastoid cells

... To allow multiple genetic analyses on a single cell, whole genome amplification (WGA) is required. Unfortunately, studies comparing different WGA methods for downstream human identification Short ...

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Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

... DNA Amplification Kit (GE Healthcare, Little Chalfont, Buckinghamshire, England); MALBAC: Yikon Genomics Single Cell Whole Genome Amplification Kit; ADO: Allele drop-out; CNVs: Copy number ...

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Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

... DNA Amplification Kit (GE Healthcare, Little Chalfont, Buckinghamshire, England); MALBAC: Yikon Genomics Single Cell Whole Genome Amplification Kit; ADO: Allele drop-out; CNVs: Copy number ...

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Highly efficient PCR assay to discriminate allelic DNA methylation status using whole genome amplification

Highly efficient PCR assay to discriminate allelic DNA methylation status using whole genome amplification

... Findings: We developed Hpa II- McrBC whole-genome-amplification PCR (HM-WGA-PCR) that uses whole-genome- amplified DNA as the template. HM-WGA-PCR uses only 1/100th the genomic template ...

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Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue     optimization for genome wide array analyses

Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue optimization for genome wide array analyses

... for genome wide analyses, such as single nucleotide polymorphism (SNP) arrays, is often greater than what can be obtained from the dissected tissue, we have compared three different whole genome ...

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Comparison of single cell sequencing data between two whole genome amplification methods on two sequencing platforms

Comparison of single cell sequencing data between two whole genome amplification methods on two sequencing platforms

... low-coverage whole genome sequencing (SLWGS) has enabled better reproducibility and accuracy for detection of copy number variations ...The whole genome amplification (WGA) method and ...

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Copy number and loss of heterozygosity detected by SNP array of formalin-fixed tissues using whole-genome amplification

Copy number and loss of heterozygosity detected by SNP array of formalin-fixed tissues using whole-genome amplification

... for whole-genome applications prohibits their use for small, laser capture micro-dissected (LCM), and/or rare clinical samples, which are also often formalin-fixed and paraffin-embedded ...(FFPE). ...

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STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods

STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods

... The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis. In these applications, target cells are extremely ...

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Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing

Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing

... Lieselot Deleye 1 , Dieter De Coninck 1 , Annelies Dheedene 2 , Petra De Sutter 3 , Björn Menten 2 , Dieter Deforce 1, * & Filip Van Nieuwerburgh 1, * Starting from only a few cells, current whole ...

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