whole-mount in situ hybridization
Multi probe in situ hybridization to whole mount Arabidopsis seedlings
... antibodies) hybridization of fluorescently la- beled nucleic acids which resolved the problems related to antibody detection through enzymatic assay per se (Bauman et ...1990s, whole mount in ...
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Original Article An optimized protocol for whole mount in situ hybridization of mouse brain
... of whole mount in situ hybridization (WMISH) which is a preferred method for transcript distributions detection in whole embryos, tissues and organs, for the detection of c-fos mRNA in ...
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Alpha tubulin marker gene of neural territory of sea urchin embryos detected by whole mount in situ hybridization
... Int J Dev lI;o! 39 477 483 (1995) 477 Q,iginal Article a Tubulin marker gene of neural territory of sea urchin embryos detected by whole mount in situ hybridization FABRIZIO GIANGUZZA', CATERINA CASAN[.] ...
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Whole mount in situ hybridization reveals the expression of the Xl Fli gene in several lineages of migrating cells in Xenopus embryos
... Int J De, lIiol 39 909 919 (1995) 909 Original Article Whole mount in situ hybridization reveals the expression of the XI Fli gene in several lineages of migrating cells in Xenopus embryos DENISE MEYE[.] ...
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Patterns of gene expression in schistosomes: localization by whole mount in situ hybridization
... used whole mount in situ hybridization (WISH) to determine gene expression patterns, as a vital tool for formulating and testing hypotheses about ...for hybridization with ...
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Localization of a nervous system specific class II beta tubulin gene in Xenopus laevis embryos by whole mount in situ hybridization
... Int I De, Riol J5 399 405 (l tJ(1) 399 Original Article Localization of a nervous system specific class II B tubulin gene in Xenopus laevis embryos by whole mount in situ hybridization RALF OSCHWALD',[.] ...
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Postimplantation mouse embryos cultured in vitro Assessment with whole mount immunostaining and in situ hybridization
... Tn! J, Dey, BioI ~1 365 3N (1997) 365 Postimplantation mouse embryos cultured in vitro Assessment with whole mount immunostaining and in situ hybridization GENEVIEVE VAN MAELE FABRY, FREDERIC CLOTMAN,[.] ...
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Mapping Neurotransmitter Identity in the Whole-Mount Drosophila Brain Using Multiplex High-Throughput Fluorescence in Situ Hybridization
... needed. Hybridization was performed in custom plexiglass chambers modified from ...20-hr hybridization reaction was car- ried out with the chambers inside a humidified polypropylene container (2249-6; Ted ...
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Rainbow trout glucose transporter (OnmyGLUT1): functional assessment in Xenopus laevis oocytes and expression in fish embryos
... The importance of facilitative glucose transporters for fish remains undefined. To study its functional properties, we expressed the putative glucose transporter OnmyGLUT1 in Xenopus laevis oocytes. This heterologous ...
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Synaptic transmission in neurons that express the Drosophila atypical soluble guanylyl cyclases, Gyc 89Da and Gyc 89Db, is necessary for the successful completion of larval and adult ecdysis
... using whole-mount in situ hybridization labeling in embryos showed that all three atypical sGC subunit genes, Gyc-89Da, Gyc-89Db and Gyc-88E, were expressed in a subset of peripheral and ...
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Toxic Eects of Paclobutrazol on Developing Organs at Di erent Exposure Times in Zebrafish
... Figure 4. Intestine defects are more severe when exposure to PBZ begins at early stages. Zebrafish embryos were exposed to a range of PBZ concentrations (0.34, 3.4, and 17 µM) beginning at different stages (24, 36, 48, ...
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FGFR1 function at the earliest stages of mouse limb development plays an indispensable role in subsequent autopod morphogenesis
... variations. Whole-mount in situ hybridization using Hoxd12 revealed a normal appearance of the first two or three digits while the development of the posterior digits was abnormal ...
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Novel bacteriocyte-associated pleomorphic symbiont of the grain pest beetle Rhyzopertha dominica (Coleoptera: Bostrichidae)
... Our whole-mount in situ hybridization data gener- ally agreed with the previous reports, in that most adult males exhibited degeneration of the bacteriomes, although we found that some adult ...
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Physiological effects of KDM5C on neural crest migration and eye formation during vertebrate development
... Molecular analyses of KDM5C functional roles using whole‑mount in situ hybridization, β ‑ galactosidase staining, and reverse transcription‑polymerase chain reaction revealed that los[r] ...
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Third generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust
... subcellular resolution within whole-mount vertebrate embryos (Trivedi et al., 2018). Precision increases with probe set size (Trivedi et al., 2018), so the prospect of using large unoptimized ...
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Evolutionary loss of melanogenesis in the tunicate Molgula occulta
... by whole-mount mRNA in situ hybridization on embryos at different developmen- tal stages ...in situ hybrid- ization using a riboprobe prepared from a genomic clone of the ...
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FGF signaling is required for initiation of feather placode development
... soaked in FGF10 (referred to as FGF10 beads) to ppf explants and assayed for changes in gene expression normally associated with the onset of bud development. In explants cultured in the presence of FGF10 beads, ...
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Mapping a multiplexed zoo of mRNA expression
... In situ hybridization methods are used across the biological sciences to map mRNA expression within intact ...in situ amplifiers based on the mechanism of hybridization chain reaction (HCR) ...
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Molecular cytogenetic identification of three rust-resistant wheat-Thinopyrum ponticum partial amphiploids
... in situ hybridization (GISH), multicolor GISH (mcGISH) and fluorescence in situ hybridization (FISH) were used to analyze the genomic constitution of SN0389, SN0398 and SN0406, three octoploid ...
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The reliability of rabbit monoclonal antibodies in the immunohistochemical assessment of estrogen receptors, progesterone receptors, and HER2 in human breast carcinomas
... in situ hybridization system (SISH) were compared with each other and with each of the antibody assays ...in situ–based systems were between the rabbit monoclonal antibodies and ...
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