WI-38 cells
Original Article Guiqi polysaccharide protects the normal human fetal lung fibroblast WI-38 cells from H
... indicated concentrations (0, 1, 10, 100, 200, 400, 800, 1600, 3200, and 6400 μmol/L) for 2 h. Then MTT assay was performed to assess the cell viability and survival rate. Our results showed that the fibroblast ...
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Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells
... HCT116 cells only; no significant changes were found in WI-38 ...in WI-38 cells; however, the original levels were restored after 48 ...HCT116 cells or the levels of ...
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Herpes Simplex Virus and Human Cytomegalovirus Replication in WI-38 Cells II. An Ultrastructural Study of Viral Penetration
... Fusion appeared to begin at one or more small foci of contact between the viral envelope and the plasmalemma, and to proceed very rapidly, since naked viral capsids free in the cytoplasm[r] ...
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Herpes simplex virus and human cytomegalovirus replication in WI-38 cells. III. Cytochemical localization of lysosomal enzymes in infected cells.
... Cytochemical Localization of Lysosomal Enzymes in Infected Cells JANET DUYCKINCK SMITH"* AND ETIENNE DE HARVEN2 Department of Anatomy, The Medical College of Pennsylvania and Hospital, P[r] ...
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Herpes Simplex Virus and Human Cytomegalovirus Replication in WI-38 Cells I. Sequence of Viral Replication
... This was true even in late stages of Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Appearance of increased numbers of perichromatinlike granules Condensation of the c[r] ...
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Oncogenicity of human papillomavirus- or adenovirus-transformed cells correlates with resistance to lysis by natural killer cells.
... (HFK-1321, WI-38 E7 clones, and H4-E7 clones) were transformed by plasmids in which E7 gene expression is driven by the IFN-insensitive ...SiHa, WI-38 E7 CL1 and CL2, and H4-E7 CL1 and ...or ...
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Differential sensitivity of normal and transformed human cells to reovirus infection.
... Because observation of infected cells indicated that trans- infection of L cells results in an inhibition of forned WI-38 cells were more sensitive to reo- cullular DNA synthesis which o[r] ...
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CYTOTOXIC NAPHTHALENE BASED-SYMMETRICAL DISELENIDES WITH INCREASED SELECTIVITY AGAINST MCF-7 BREAST CANCER CELLS
... Interestingly, significant difference in toxicity zones between breast solid tumor cells and normal WI-38 cells was noticed. In this context, the TI values of cytotoxic compounds 2, 9, 10, 12, ...
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Nonproductive Infection of Guinea Pig Cells with Human Cytomegalovirus
... GP and WI-38 cells were infected with 1.5 x 105 TCID50/0.2 ml, and, after 1 h of adsorption, cultures were washed and treated with rabbit anti-CMV serum or with preimmune serum.. The cul[r] ...
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Human cytomegalovirus stimulates host cell RNA synthesis.
... 72 48 24 HOURS AFTER INFECTION 48 24 HOURS AFTER 72 INFECTION Comparison of the rate of labeling of nuclear and cytoplasmic RNA between CMV-infected WI-38 cells in the presence - and abs[r] ...
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Proteome alteration induced by hTERT transfection of human fibroblast cells
... type WI 38 cells (WI38), hTERT transfected WI 38 cells (WI38-hTERT) and control HPV transfected WI 38 cells (WI38-HPV) har- vested at the same number of ...
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Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma
... 1c1c7) cells, as under these experimental conditions murine AHR is more stable than the human counterpart ...in WI-38 cells (Supplemental Figure ...in WI-38 control cells, ...
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Middle East Respiratory Syndrome Coronavirus Infection Mediated by the Transmembrane Serine Protease TMPRSS2
... and WI-38 ...MRC-5, WI-38, and FIG 5 Inhibition of syncytium formation and S-protein degradation by ...Vero-TMPRSS2 cells were infected with MERS-CoV at an MOI of ...the cells ...
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Long non-coding RNA MALAT1 protects preterm infants with bronchopulmonary dysplasia by inhibiting cell apoptosis
... in WI-38 cells induces cell ...A549 cells verified WI-38 ...A549 cells apoptosis analyzed by flow cytometry showed that, after MALAT1 was knocked down, apoptosis in A549 ...
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Practical protocol for cytomegalovirus isolation: use of MRC 5 cell monolayers incubated for 2 weeks
... In the second segment, one tube of WI-38 cells was compared with one tube of MRC-5 cells over a 14-day period for susceptibility to CMV infection and speed of development of viral cytopa[r] ...
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Bradykinin stimulates NF kappaB activation and interleukin 1beta gene expression in cultured human fibroblasts
... possibility, WI-38 cells were transiently transfected with chimeric reporter plasmids before BK stimu- ...in cells transfected with a reporter plasmid containing the kB enhancer from the IkB ...
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Tumor-Specific, Replication-Competent Adenovirus Vectors Overexpressing the Adenovirus Death Protein
... proliferating cells in the body. The vectors rep- licated in HEL-299 and WI-38 cells growing in 10% serum, but it is unlikely that these cells are truly ...of cells to divide is ...
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Immunoperoxidase technique for detection of antibodies to human cytomegalovirus
... The specificity was demonstrated by: i the inability of CMV-positive human serum to stain V-Z- and HSV-infected and uninfected WI-38 cells, using a 1:20 or greater dilution of conjugate;[r] ...
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Discovery of cell surface vimentin targeting mAb for direct disruption of GBM tumor initiating cells
... To analyze protein expression, cells were lysed in radioimmunoprecipitation assay (RPPA) buffer (50 mM Tris-HCl, pH 7.4; 1% NP-40; 0.25% sodium deoxycholate; 150 mM NaCl; 1 mM EDTA) supplemented with a complete ...
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Herpangina and Pleurodynia Associated with Herpes simplex Virus
... Herpes simplex virus was isolated from the oral lesions in WI-38. tissue culture and identified by neutralization[r] ...
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