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Wild type enzyme

Purification and characterization of mutant miniPlasmin for thrombolytic therapy

Purification and characterization of mutant miniPlasmin for thrombolytic therapy

... the wild-type mPlg was activated without further purification, only about 25% is in active ...the wild type enzyme, and therefore may be more susceptible to non-specific cleav- ...

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Computer simulations of the catalytic mechanism of wild-type and mutant β-phosphoglucomutase

Computer simulations of the catalytic mechanism of wild-type and mutant β-phosphoglucomutase

... the wild-type enzyme, the T16 side chain forms a hydrogen bond with the carboxylic group of D10 and is thus important for positioning the D10 side ...the wild-type ...the enzyme ...

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Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase.

Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase.

... Because wild-type NPH-II does display a low basal rate of NTP hydrolysis without a cofactor (this applies to the native enzyme from virions as well as the recombinant protein), it is axiomatic that ...

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Direct measurement of the substrate preference of uracil DNA glycosylase

Direct measurement of the substrate preference of uracil DNA glycosylase

... the wild-type enzyme was tested, no binding was observed to oligonucleotides ssC or ...active enzyme making it impossible to detect a stable enzyme z DNA ...mutant enzyme before ...

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Do deleterious mutations act synergistically? Metabolic control theory provides a partial answer.

Do deleterious mutations act synergistically? Metabolic control theory provides a partial answer.

... If fitness is directly proportional to flux, and the wild- type enzyme acts close to its highest possible perform- ance in the pathway, synergistic interaction among mut[r] ...

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STRUCTURE, FUNCTION AND EVOLUTION OF Serratia marcescens ENDONUCLEASE

STRUCTURE, FUNCTION AND EVOLUTION OF Serratia marcescens ENDONUCLEASE

... the wild type enzyme, demonstrating that the two subunits in wild type Serratia nuclease function independently to each other; however, at very low enzyme and substrate ...

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Improved conversion of ginsenoside Rb1 to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase

Improved conversion of ginsenoside Rb1 to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase

... variant enzyme for other glycosides, the wild-type and W361F variant enzymes were used for the hydrolysis of two flavanone glyco- sides, naringin and ...variant enzyme for hesperidin was ...

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A Cell Cycle-Regulated Toxoplasma Deubiquitinase, TgOTUD3A, Targets Polyubiquitins with Specific Lysine Linkages

A Cell Cycle-Regulated Toxoplasma Deubiquitinase, TgOTUD3A, Targets Polyubiquitins with Specific Lysine Linkages

... recombinant wild-type TgOTUD3A or mutant TgOTUD3A (C229A) enzyme in a 30-min reaction, resolved using SDS-PAGE, transferred, and probed with linkage-specific ...the wild type but not ...

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Characterization of a soluble stable human cytomegalovirus protease and inhibition by M-site peptide mimics.

Characterization of a soluble stable human cytomegalovirus protease and inhibition by M-site peptide mimics.

... the wild-type mature HCMV protease (amino acids 1 to 256) is expressed in ...combinant wild-type enzyme expressed in ...of enzyme for structural studies and inhibitor screening, ...

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NKeith Honors Thesis (CDR).pdf

NKeith Honors Thesis (CDR).pdf

... All of the mutant forms of PLCg1 found in ATL had increased basal phospholipase activity, as compared to the wild-type enzyme after transient over-expression in HEK293 cells (Figure 3). This increase ...

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STUDIES OF ADENYLOSUCCINASE IN MUTANTS AND REVERTANTS OF NEUROSPORA CRASSA

STUDIES OF ADENYLOSUCCINASE IN MUTANTS AND REVERTANTS OF NEUROSPORA CRASSA

... On the other hand, the primary F12 reverse mutant having 25 percent of wild type enzyme activity gave rise (through secondary mutant Mi ) to secondary revertants possessing [r] ...

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Combined effect of regulatory polymorphisms on transcription of UGT1A1 as a cause of Gilbert syndrome

Combined effect of regulatory polymorphisms on transcription of UGT1A1 as a cause of Gilbert syndrome

... Effect of regulatory polymorphisms on transcription Based on haplotype analysis of Gilbert patients in Japa- nese and Caucasians, we selected the most typical regula- tory regions of wild and Gilbert-syndrome ...

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Hydroxylation of diverse flavonoids by CYP450 BM3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities

Hydroxylation of diverse flavonoids by CYP450 BM3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities

... Flavonoids are one of the most numerous and struc- turally diverse natural products present in the plant kingdom [16]. They are known to have multi-beneficial medicinal and chemo-preventive activities in human health. ...

15

G140S/Q148R and N155H mutations render HIV 2 Integrase resistant to Raltegravir whereas Y143C does not

G140S/Q148R and N155H mutations render HIV 2 Integrase resistant to Raltegravir whereas Y143C does not

... been suggested that the genetic barrier is weaker in HIV-2, potentially resulting in the more rapid emer- gence of resistance to other PIs [8,9]. The development of novel treatments based on drug classes highly effec- ...

10

Indian hedgehog couples chondrogenesis to osteogenesis in endochondral bone development

Indian hedgehog couples chondrogenesis to osteogenesis in endochondral bone development

... Analysis of BrdU incorporation. Pregnant mice were injected intraperitoneally with 50 µ g BrdU per gram body weight 1 hour before sacrifice. Embryos were treated to generate sections as already described here. BrdU was ...

11

Cationic nanoparticles directly bind angiotensin-converting enzyme 2 and induce acute lung injury in mice

Cationic nanoparticles directly bind angiotensin-converting enzyme 2 and induce acute lung injury in mice

... in wild-type mice and angiotensin-coverting enzyme 2 (ACE2) knockout mice by the intratracheal instillation of cationic polyamidoamine dendrimer (PAMAM) ...II type I receptor antagonist, can ...

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Extracellular expression of a novel β-agarase from Microbulbifer sp. Q7, isolated from the gut of sea cucumber

Extracellular expression of a novel β-agarase from Microbulbifer sp. Q7, isolated from the gut of sea cucumber

... To determine the hydrolytic pattern of the recombinant agarase, a time course of its activity was carried out by TLC. The agarase initially produced agarose-oligosac- charides of various lengths, which were progressively ...

9

FOLFIRI plus panitumumab in the treatment of wild type KRAS and wild type NRAS metastatic colorectal cancer

FOLFIRI plus panitumumab in the treatment of wild type KRAS and wild type NRAS metastatic colorectal cancer

... and wild-type NRAS mCRC patients followed up at the medical oncology department of the Okmeydani Training and Research Hospital in Istanbul, Turkey, between April 2014 and January 2018 were enrolled in this ...

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Isolation and Characterization of the Xanthine Dehydrogenase Gene of the Mediterranean Fruit Fly, Ceratitis capitata

Isolation and Characterization of the Xanthine Dehydrogenase Gene of the Mediterranean Fruit Fly, Ceratitis capitata

... between wild-type and rosy medfly extracts is the result of slight gel loading variation in the absence of predefined sample ...in enzyme activity between wild-type and rosy medfly ...

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Thiol-Independent Action of Mitochondrial Thioredoxin To Support the Urea Cycle of Arginine Biosynthesis in Schizosaccharomyces pombe

Thiol-Independent Action of Mitochondrial Thioredoxin To Support the Urea Cycle of Arginine Biosynthesis in Schizosaccharomyces pombe

... the wild-type cells through pREP41- and pREP42- based expression system, ...drial enzyme (a putative glycerol dehydrogenase; Gld1) that is similarly tagged at C terminus with ...

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