wild type HIV-1 protease
AN IN SILICO STUDY ON HIV 1 PROTEASE WILD TYPE AND MUTANT WITH INHIBITORS FROM ANNONA SQUAMOSA
... thrive. HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV, the retrovirus that causes ...AIDS. HIV Wild ...
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Maintenance of the Gag/Gag-Pol Ratio Is Important for Human Immunodeficiency Virus Type 1 RNA Dimerization and Viral Infectivity
... virus type 1 (HIV-1) Gag-Pol precursor protein results from a ⴚ 1 ribosomal frameshifting ...on HIV-1 repli- cation and viral assembly, we altered the Gag/Gag-Pol ratio in ...
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In virio SHAPE analysis of tRNALys3 annealing to HIV 1 genomic RNA in wild type and protease deficient virus
... in wild type viruses [17, ...from wild type or Pr- viruses that correlate with these ...in protease-negative virions than in wild type vi- rions (17, ...
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Altered Substrate Specificity of Drug-Resistant Human Immunodeficiency Virus Type 1 Protease
... and HIV PRs 945 and 1049 each had a V82A ...in HIV PR 945 was reverted to the wild-type residue, ...variant, HIV PR 945/A82V, was assayed using both the P1-Met library and the ...
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Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells
... of protease activity and/or autoprocessing ...pseudo wild-type HIV protease construct (Figure 2B lane 13), whereas VLPs produced by the D25N mutant contained only the full length Gag ...
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Cysteine 95 and other residues influence the regulatory effects of Histidine 69 mutations on Human Immunodeficiency Virus Type 1 protease autoprocessing
... pseudo wild type protease sequence abolishes protease autoproces- sing in ...conserved HIV-1 protease cysteines are not required for the catalytic activity, nor ...
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High Frequency of Non-B Human Immunodeficiency Virus Type 1 (HIV-1) Subtype Specific Mutations at the Protease Gene among Treatment-naive HIV-1 Infected Individuals in Jos, Nigeria
... from HIV infection globally ...the HIV infected population [12]. The Nigerian national guidelines for HIV/AIDS treatment and care in adolescent and adults recommended boosted protease ...
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Identification of Biased Amino Acid Substitution Patterns in Human Immunodeficiency Virus Type 1 Isolates from Patients Treated with Protease Inhibitors
... in HIV-1 subtype B protease from un- treated ...that HIV-1 variants with every possible amino acid substitution are produced daily in HIV-1- infected individuals ...those ...
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Defining the level of human immunodeficiency virus type 1 (HIV-1) protease activity required for HIV-1 particle maturation and infectivity.
... in wild-type virions (8, 29, ...that protease inhibitor-treated viruses containing decreased amounts of pro- cessed polyproteins and reverse transcriptase activity are not able to direct efficient ...
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Sources and Magnitude of Intralaboratory Variability in a Sequence Based Genotypic Assay for Human Immunodeficiency Virus Type 1 Drug Resistance
... virus type 1 (HIV-1) protease (PR) and reverse transcriptase (RT) by using replicate subanalyses of 46 plasma samples collected from HIV-1-infected, ...
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Human immunodeficiency virus type 1 (HIV-1) protein Vif inhibits the activity of HIV-1 protease in bacteria and in vitro.
... virus type 1 (HIV-1) encodes auxiliary proteins which are not found in oncoretroviruses but which influence HIV-1 replication ...among HIV-1-infected per- sons (20, ...
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Characterization of human immunodeficiency virus type 1 dimeric RNA from wild-type and protease-defective virions.
... In an effort to determine whether the dimers of full-length genomic RNA extracted from HIV-1 particles exhibit this property, we compared the thermal stability of HIV-1 dimeric RNAs in 1[r] ...
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Intracellular expression of human immunodeficiency virus type 1 (HIV-1) protease variants inhibits replication of wild-type and protease inhibitor-resistant HIV-1 strains in human T-cell lines.
... prevent HIV-1 replication in individual cells are ...efficacies, HIV-1 replication occurs only in cells which do not express effective td PR ...on HIV-1 is minimal or nonexistent ...
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High Degree of Interlaboratory Reproducibility of Human Immunodeficiency Virus Type 1 Protease and Reverse Transcriptase Sequencing of Plasma Samples from Heavily Treated Patients
... drug-resistant HIV-1 variants is ...reported protease codon 90 for patient PCC25 and protease codon 48 for patient PCC26 as being of the wild type (WT) because the mutant (Mut) ...
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In vitro selection and characterization of human immunodeficiency virus type 1 (HIV-1) isolates with reduced sensitivity to hydroxyethylamino sulfonamide inhibitors of HIV-1 aspartyl protease.
... that HIV-1 protease has the plasticity to sustain multiple sequence changes that result in reduced in- hibitor binding but not elimination of catalytic ...in HIV-1 protease that ...
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Construction of a Human Immunodeficiency Virus Type 1 (HIV-1) Library Containing Random Combinations of Amino Acid Substitutions in the HIV-1 Protease due to Resistance by Protease Inhibitors
... 30 protease recombinants of the HIV-1 pNG-PRL ...from HIV-1-infected MT-2 ...viral protease-encoding sequence was amplified by PCR with primers PR5 (5⬘-GTT AAG TGT TTC AAT TGT ...
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Receptor-Triggered but Alkylation-Arrested Env of Murine Leukemia Virus Reveals the Transmembrane Subunit in a Prehairpin Conformation
... sponding HIV-1 gp41 peptide (16 M) ...the HIV-1 gp41 and avian leukosis virus TM ...not HIV-1, the LAS was found to be resistant to peptide inhibition (7, 17, 19, ...
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Analysis of the mechanical properties of wild type and hyperstable mutants of the HIV 1 capsid
... ΔEnv HIV-1 genome vector containing either the WT or E45A CA mutant sequence, using a method previ- ously described ...concentrated HIV-1 pseudovi- ral particles with an equal amount of ...
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HIV protease inhibitors: a review of molecular selectivity and toxicity
... of HIV, 26 anti-HIV compounds have been approved by the US Food and Drug Administration ...are HIV protease inhibitors. The combination therapy of HIV protease inhibitors, ...
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Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Nuclear Localization Mediates Early Viral mRNA Expression
... for HIV-1 spliced mRNA and cellular -globin mRNA were as previously described (22, 28, ...for HIV-1 NC localization, were examined for early viral spliced mRNA ...
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