X-Gal
The lacI Gene as a Target for Mutation in Transgenic Rodents and Escherichia coli
... Figure 2.—Numbering starts at 1 at the mRNA transcription start. The preceding nucleotide is 21 (there is no “0”). The translation start is at position 29–31. The initiation codon is GTG, which codes for Val, but is read ...
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Circling behavior developed in Dmp1 null mice is due to bone defects in the vestibular apparatus
... and X-gal stain assay performed in this current study failed to display DMP1 signals in neuron ...with X-gal due to a lack of specific pericyte ...
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Wnt signaling mediates regional specification in the vertebrate face
... In our next experiments, E9.5 embryos were infected with Ad- Dkk1 and then harvested 96 hours later. First, we found no differences in the size of control and Ad-Dkk1-treated embryos (Fig. 6, compare D with F), which ...
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Establishment of markerless gene deletion tools in thermophilic Bacillus smithii and construction of multiple mutant strains
... subjected again to colony PCR after overnight growth. One colony showing a strong double crossover knockout band was transferred to LB2 containing 50 mM ammo- nium acetate and grown overnight, after which it was ...
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Transgenic mice exhibiting inducible and spontaneous Cre activities driven by a bovine keratin 5 promoter that can be used for the conditional analysis of basal epithelial cells in multiple organs
... mount X-gal staining revealed the presence of intensive blue-stained areas in the esophagus (E) and forestomach (FS; arrows) of K5-R26R ...mount X-gal staining and his- tological section and ...
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Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors
... Lysates from the CTY10-5d yeast strain bearing lexA MLV integrase (pSH2-1 and pNlexA) constructs were examined for protein expression on Western blots probed with an anti-LexA antibody (Figure 1A). To examine potential ...
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Infection of Nondividing Cells by Rous Sarcoma Virus
... X-Gal staining of HT1080 cells infected with the different viral vectors revealed that the presence of aphidicolin did not affect HIV-1 titers but reduced RSV and, to a greater extent, MLV titers compared ...
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Extracellular domain of CD98hc is required for early murine development
... embryos, X-gal staining revealed a ratio of 1 to 4 for the wild type embryos to homozygous plus heterozy- gous mutants, suggesting that homozygous mutant sur- vived up to this ...
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Abnormal hair follicle development and altered cell fate of follicular keratinocytes in transgenic mice expressing ΔNp63α
... Dorsal skin sections of triple transgenic animals stained with X-Gal reveal a dramatic reduction of Wnt activity in the hair follicle matrix cells as compared with control animals, sugge[r] ...
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Collagen induced arthritis and imiquimod induced psoriasis develop independently of interleukin 33
... Background: Interleukin (IL)-33 is a dual cytokine with both an alarmin role and a T helper 2 cell (Th2)-like inducing effect. It is involved in the pathogenesis of rheumatoid arthritis (RA) and its models; we recently ...
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Distinct Roles of Adenovirus Vector-Transduced Dendritic Cells, Myoblasts, and Endothelial Cells in Mediating an Immune Response against a Transgene Product
... matory context leading to a higher response. Kinetic analyses showed that animals injected with AdGal-transduced EC de- veloped an antibody response at least 7 days earlier than those injected with ...
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Endothelial cell derived semaphorin 3A inhibits filopodia formation by blood vascular tip cells
... Fig. 1. Sema3a mRNA is expressed in the retinal ganglion cell layer and the secreted protein binds to Nrp1- and plexin-A1- positive blood vessels. (A) X-gal-stained retinal whole mount of a P7 Sema3a +/lacZ ...
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Expression of Plxdc2/TEM7R in the developing nervous system of the mouse
... Expression of Plxdc2- geo in the E15.5 brain was inves- tigated by X-gal staining of 100 m sections from heterozy- gous Plxdc2 gene trap mice (Fig. 5). An intricate pattern of Plxdc2- geo expression was ...
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Isolation and characterization of a mutant of Saccharomyces cerevisiae with pleiotropic deficiencies in transcriptional activation and repression.
... It has recently become apparent (M. Color development was scored after two days of growth on plates containing X-gal. The biological sig- nificance of this coordinated [r] ...
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Restoration of tubular epithelial cells during repair of the postischemic kidney occurs independently of bone marrow derived stem cells
... by X-gal staining, but following suppression of endogenous (mammalian) b-gal, no tubular cells could be found that stained with X-gal after ischemic ...
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Introductory Experiments in Recombinant DNA
... containing X-gal and IPTG are transferred to a nutrient plate containing tetracycline at a concentration of 20 µg/ml, the blue colonies containing pUC19 will be unable to grow and the white colonies ...
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Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae.
... 1943; LEA and COULSON 1949), as described in MA- T o determine whether strains that formed blue colonies on X-gal plates containing plasmids with an alteration in [r] ...
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α-Galactosidase A-Tat Fusion Enhances Storage Reduction in Hearts and Kidneys of Fabry Mice
... We have previously shown that neo- natal injection of recombinant LVs into Fabry mice encoding the α -gal A cDNA led to sustained systemic expression of the therapeutic transgene (10) and re- ductions in Gb3 ...
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Original Article Overexpression of the galectin-3 during tumor progression in prostate cancer and its clinical implications
... of gal-3 modulated angiogenesis, tumor growth and was associated with malig- nancy in primary lesions [8, ...cleaved gal-3 was implicated in cell migration and bone metastasis [6, 10, ...that gal-3 ...
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A carboxy terminal truncation of human alpha galactosidase A in a heterozygous female with Fabry disease and modification of the enzymatic activity by the carboxy terminal domain Increased, reduced, or absent enzyme activity depending on number of amino acid residues deleted
... an X-linked disorder of glycosphingolipid me- tabolism caused by a deficiency of a -galactosidase A ( a -Gal ...words: X-linked disorder • de novo mutation • one base insertion ...
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