Xenopus laevis oocytes
Properties of the mammalian and yeast metal ion transporters DCT1 and Smf1p expressed in Xenopus laevis oocytes
... in Xenopus laevis oocytes, exhibited a much lower activity than its mammalian homologue DCT1 (Chen et ...SMF3. Xenopus laevis oocytes expressing the chimeric Smf1p exhibited an ...
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Characterization of an endogenous Na+/H+ antiporter in Xenopus laevis oocytes
... The effect of sodium concentration on amiloride-sensitive ^Na"1" uptake by defolliculated Xenopus laevis oocytes.. Data from four experiments were normalized to a value of 1.0at20mmoll~1[r] ...
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Development of selective blockers for Ca2+-activated Cl- channel using Xenopus laevis oocytes with an improved drug screening strategy
... solution in attempt to prevent activation of endogenous CaCCs. These CaCCs have similar properties in many ways to those in cardiac muscle, smooth muscle, secretory epithelial cells and neurons [13]. There have been a ...
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Expression of membrane targeted aequorin in Xenopus laevis oocytes
... Int J [Jev Bioi 39 653 657 (1995) 653 Short Contribution Expression of membrane targeted aequorin in Xenopus laevis oocytes CHRISTIANE DAGUZAN', MARIE THERESE NICOLAS2, CHRISTIAN MAZARS3, CATHERINE LE[.] ...
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Translation of ovalbumin mRNA in Xenopus laevis oocytes Characterization of the system and effects of estrogen on injected mRNA populations
... into Xenopus laevis oocytes. The oocytes were incubated in culture medium containing ...microinjected oocytes over a minimum period of 91 ...
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Rainbow trout glucose transporter (OnmyGLUT1): functional assessment in Xenopus laevis oocytes and expression in fish embryos
... The importance of facilitative glucose transporters for fish remains undefined. To study its functional properties, we expressed the putative glucose transporter OnmyGLUT1 in Xenopus laevis oocytes. ...
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Distribution of exchanges upon homologous recombination of exogenous DNA in Xenopus laevis oocytes.
... continuous through the homologous segment, and the Because oocyte recombination proceeds via a single- distribution of restriction sites indicates which parental strand annea[r] ...
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Expression of the hepatocellular chloride dependent sulfobromophthalein uptake system in Xenopus laevis oocytes
... in oocytes represents a promising approach to ultimately clone the cDNA coding for the hepatocyte high affinity, chloride-dependent organic anion uptake ...
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Heterogeneous nuclear ribonucleoprotein complexes from Xenopus laevis oocytes and somatic cells
... In order to facilitate the study of the candidate RNA-binding proteins described above, we used proteins eluted with 2.0M NaCl from ssDNA-cellulose as antigen for production of monoclonal antibodies. Several hybridomas ...
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Repair of heteroduplex DNA in Xenopus laevis oocytes.
... As part of our characterization of the mechanism of homologous recombination in Xenopus oocytes, we un- dertook a study of the distribution of multiple heteroal- lelic markers in [r] ...
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Rabies mRNA translation in Xenopus laevis oocytes.
... In this report we present evidence that two rabies mRNA's analyzed thus far show individual specificity for synthesis of: i the rabies surface glycoprotein G protein and ii the 133 Downl[r] ...
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Selecting karyophilic DNA cis elements in Xenopus laevis oocytes; a new approach
... The asymmetry which we consistently observed in the selection for nuclear import versus nuclear retention, could reflect not only the different DNA-interacting protein compositions in cytoplasm and nucleus, but also the ...
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Centroid, a novel putative DEAD box RNA helicase maternal mRNA, is localized in the mitochondrial cloud in Xenopus laevis oocytes
... In Xenopus species, the early stages of oogenesis take place in the developing tadpole ovary when the oocytes are in a period critical for the organization of the germ plasm (believed to be a determinant of ...
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The action of the v ErbA oncogene within the regulatory and structural networks of Xenopus laevis oocytes
... Interestingly, v-ErbA acted as an efficient constitutive activator at rGH sTRE in the presence of nuclear protein extract from anterior pituitary cells, whereas it had no effect on basal[r] ...
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Orthopoxvirus gene expression in Xenopus laevis oocytes: a component of the virion is needed for late gene expression.
... Injection of oocytes with subviral cores resulted in only early viral protein synthesis, whereas injection of intact virions allowed additional expression of late viral gene products.. W[r] ...
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mRNA- and DNA-directed synthesis of herpes simplex virus-coded exonuclease in Xenopus laevis oocytes.
... This substrate was incubated with DNA 386 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Microinjection of herpes simplex virus HSV-infected cell mRNA into Xenopus lae[r] ...
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Translation of three mouse hepatitis virus strain A59 subgenomic RNAs in Xenopus laevis oocytes.
... This polypeptide had no counterpart in the virion, but comigrated with a virus-specific glycoprotein present in infected cells which is immunoprecipitated by a rabbit antiserum against t[r] ...
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Amino acid dependent modulation of amino acid transport in Xenopus laevis oocytes
... in oocytes after (A) transfer from amino-acid-deprived to amino-acid- supplemented culture medium and (B) transfer to amino-acid- deprived medium ± cycloheximide after 24 h of preincubation in ...
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Differential patterns of inhibition of the sugar transporters GLUT2, GLUT5 and GLUT7 by flavonoids
... by Xenopus laevis oocytes expressing ...microinjection, oocytes were incubated in ...(18 oocytes) normalized to respective water-injected controls, incubated in the same ...
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Analysis of proteins bound to stored messenger RNA in 'Xenopus' oocytes
... Different forms of soluble cytoplasmic mRNA binding proteins and particles in Xenopus laevis oocytes and embryos.. Sequence analysis of cytoplasmic mLNA-binding proteins of Xenopus oocyt[r] ...
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