Xylose isomerase
Systematic and evolutionary engineering of a xylose isomerase-based pathway in Saccharomyces cerevisiae for efficient conversion yields
... on xylose increased progressively for the exponential phase transfer ...on xylose by using Bioscreen C for high throughput ...on xylose compared to those from the medium and high inoculum sizes (1% ...
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Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24
... D-xylose isomerase genes from Bacteroides and Prevotella ...D-xylose isomerase genes were isolated from three Bacteroides ...D-xylose isomerase genes from other ...
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Ethanol production with β-xylosidase, xylose isomerase, and Saccharomyces cerevisiae from the hydrolysate of Japanese beech after hot-compressed water treatment
... initial xylose was assumed to be involved in this process, where additional xylose was supplied to the following reactions as mentioned in ...of xylose to xylulose produced was converted and that a ...
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Disruption of PHO13 improves ethanol production via the xylose isomerase pathway
... in xylose assimilation ...from xylose by a strain expressing the xylose reductase (XR) and xylitol dehydrogenase (XDH) genes; however, the yield of ethanol is ...the xylose isomerase ...
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Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways
... evaluated in yeast cell extracts after 24 h of xylose fer- mentation at 30 and 40 °C (Fig. 3). At 30 °C, the PE-XR/ XDH showed the highest activity among all the strains of the XR (both using NADH and NADPH ...
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Screening and evolution of a novel protist xylose isomerase from the termite Reticulitermes speratus for efficient xylose fermentation in Saccharomyces cerevisiae
... 5‑phosphate isomerase; RPE1, ribulose 5‑phosphate epimerase; GND2, 6‑phosphogluconate dehydrogenase; SOL4, 6‑phosphogluconolactonase; ZWF1, glucose‑6‑phosphate dehydrogenase; HXT1, hexose transporter 1; HXT5, ...
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Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae
... A list of strains used in this study is shown in Additional file 1: Table S1. Eight currently known XI genes (xylA) derived from microorganisms were expressed in yeast and the ability of the transformants to metabolize ...
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Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant Saccharomyces cerevisiae
... the xylose fermentation capacity of the RWB217 must be ascribed to unknown strain ...of xylose fermentation with the isogenic strains demonstrates that the Piromyces XI activity is not sufficient to ensure ...
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Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains
... and xylose, which in some raw materials such as agricultural residues and hardwoods, can account for more than 30% of total sugars [9], and which consti- tutes a significant barrier to the cost-effectiveness and ...
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Systematic optimization of gene expression of pentose phosphate pathway enhances ethanol production from a glucose/xylose mixed medium in a recombinant Saccharomyces cerevisiae
... of xylose isomerase (XI) and evaluated xylose consumption and etha- nol production of these yeast transformants in glucose/xylose mixed media at 36 ...of xylose consumption relative to ...
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Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli
... However, xylose is the second most abundant sugar in nature, and accounts for 18–30% of sugar in lignocellulose ...metabolize xylose is a desirable attribute of microorganisms for optimizing the economics ...
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Prefermentation improves xylose utilization in simultaneous saccharification and co-fermentation of pretreated spruce
... encoding xylose isomerase (XI) from bacteria and fungi [4,5], or genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) from fungi [6,7] can be intro- ...that xylose transport ...
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Development of a strictly regulated xylose-induced expression system in Streptomyces
... inducer. Xylose isomerase (xylA) promoter and xylose (xyl) operon were chosen in an initial attempt to investigate induced protein expression in a Streptomyces strain using various carbon ...
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Genomic and phenotypic characterization of a refactored xylose-utilizing Saccharomyces cerevisiae strain for lignocellulosic biofuel production
... of xylose-utilizing strains of Saccha- romyces cerevisiae has improved the prospects of ligno- cellulosic biorefinery, enabling the creation of full-scale second-generation bioethanol production plants world- wide ...
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Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha
... fuels. Xylose represents a significant constituent of the hemicellulose xylan fraction of ...ferment xylose efficiently to ethanol to improve process econom- ics for the production of this renewable liquid ...
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L-lactic acid production from D-xylose with Candida sonorensis expressing a heterologous lactate dehydrogenase encoding gene
... with xylose isomerase [26], the best L-lactic acid production was detected in strains from which both xylose reduc- tase and xylitol dehydrogenase encoding genes had been deleted and which also ...
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Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective
... one‑step isomerase pathway using xylose isomerase have been employed to enable xylose assimilation in engi‑ neered ...of xylose fermentation, expression levels of xylose ...
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Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast
... is xylose resistant retain- ing its 50 % of activity in presence of 300 mM xylose ...by xylose and ethanol proved that this enzyme is a poten- tial candidate to be used in biotechnological processes ...
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Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization
... XR reaction. The lowest flux towards oxalacetate (< 0.10 mol/mol sugar) was calculated for the condition of an NADH-specific XR. Wahlbom et al. used S. cerevisiae strain TMB 3001, which is similar to our strain BP000 ...
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A xylose-stimulated xylanase–xylose binding protein chimera created by random nonhomologous recombination
... by xylose was used to detect XBP ...by xylose in a concentration-dependent response ...for xylose uptake by ...The xylose-uptake deficient ...the xylose inducible promotor of the xylF ...
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