Xylose Reductase
A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw
... heterologous xylose pathway including either the native xylose reductase (XR) from ...The xylose conversion in SSCF was doubled with the ...the xylose uptake, and did not increase the ...
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Effect of Dissolved Oxygen and Inoculum Concentration on Xylose Reductase Production from Candida guilliermondii Using Sugarcane Bagasse Hemicellulosic Hydrolysate
... of xylose reductase, interfering significantly in this process, as well as its combination with an appropriate percentage of dissolved ...of xylose reductase and the feasibility of enzymatic ...
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Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae
... Rising oil prices and a growing awareness of a possible cli- mate change caused by greenhouse gas emission have recently led to rekindled interest in bioethanol as a CO 2 - neutral liquid fuel. Lignocellulose will be the ...
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Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha
... fuels. Xylose represents a significant constituent of the hemicellulose xylan fraction of ...ferment xylose efficiently to ethanol to improve process econom- ics for the production of this renewable liquid ...
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Preliminary Characterization of Xylose Reductase Partially Purified by Reversed Micelles from Candida tropicalis IEC5 ITV, an Indigenous Xylitol Producing Strain
... Extraction by reverse micelles is a useful versatile tool for separating biomolecules such as enzymes. This me- thod involves the use of ternary mixtures composed of water-surfactant-solvent which in a first step ...
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Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing
... utilize xylose efficiently, while D- xylulose, an isomerization product of D-xylose, can be ...constructing xylose-fer- menting ...in xylose metabolism from other organisms. Xylose ...
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Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways
... convert xylose during cotton stopper fermentation in synthetic medium ...similar xylose consumption profiles (at a 95% con- fidence level); however, the PE-2∆GRE3 containing only the XI pathway was capable ...
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Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization
... of xylose by engineered strains of ...that xylose uptake was enhanced at low concentrations of glucose as compared to otherwise identical reaction conditions lacking glucose ...of xylose consumption ...
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Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
... expression with a standard assay (200 μM NAD(P)H, 350 mM xylose) (Table 3). Y-PsK270M displayed only about 34% and 36% of the NADPH and NADH-dependent XR activities compared with the reference strain Y-PsNative. ...
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Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans
... the xylose-consuming capac- ity of the parent strain was markedly inhibited ...residue xylose in the fermentation broth of the parent strain and P4 mutant were ...mum xylose consumption rate of P4 ...
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Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant Saccharomyces cerevisiae
... the xylose fermentation capacity of the RWB217 must be ascribed to unknown strain ...of xylose fermentation with the isogenic strains demonstrates that the Piromyces XI activity is not sufficient to ensure ...
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Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF)
... converts xylose, but also displays higher tolerance to lignocellulosic hydrolysate during anaerobic batch fermentation as well as 3 times higher in vitro HMF and furfural reduction activity than the control strain ...
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Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana
... Background: The heterotrophic and mixotrophic culture of oleaginous microalgae is a promising process to produce biofuel feedstock due to the advantage of fast growth. Various organic carbons have been explored for this ...
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Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains
... To the best of our knowledge, arabinose and xylose co- metabolism in recombinant S. cerevisiae strains has previ- ously only been reported for one recombinant industrial strain of S. cerevisiae [27]. In this ...
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Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli
... Enzyme-catalyzed enantioselective reductions of ketones have become quite popular for the production of homo- chiral alcohols at industrial scale [1]. NAD(P)H-depend- ent reductases catalyze these transformations with ...
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Screening and evolution of a novel protist xylose isomerase from the termite Reticulitermes speratus for efficient xylose fermentation in Saccharomyces cerevisiae
... of xylose by ...of xylose, cata- lyzed by xylose reductase (XR) and xylitol dehydrogenase (XDH), as a result of which d-xylulose is ...
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Engineering xylose utilization in Yarrowia lipolytica by understanding its cryptic xylose pathway
... a xylose growth challenge strategy using ...native xylose gene (xylA or xylB) and forcing ...and xylose, ...of xylose reductase ...
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Cross-reactions between engineered xylose and galactose pathways in recombinant Saccharomyces cerevisiae
... Similarly, xylose utilization has been improved by overexpression of genes encoding xylulokinase (XK), enzymes from the non-oxidative pentose phosphate pathway (non-ox PPP) and deletion of the endogenous aldose ...
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Prefermentation improves xylose utilization in simultaneous saccharification and co-fermentation of pretreated spruce
... encoding xylose isomerase (XI) from bacteria and fungi [4,5], or genes encoding xylose reductase (XR) and xylitol dehydrogenase (XDH) from fungi [6,7] can be intro- ...that xylose transport ...
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Phenotypic studies of pho13Δ in Saccharomyces cerevisiae
... D- xylose used transposon mutagenesis and identified beneficial pho13 ...ferment xylose to ethanol is an important step in the development of biofuel sustainability as xylose comprises approximately ...
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