yeast cells
Transcriptional Activation in Yeast Cells Lacking Transcription Factor IIA
... deplete yeast cells of Toa1, the large subunit of TFIIA, to ,1% of the wild- type ...TFIIA-depleted cells are essentially unaffected for activation by heat shock factor, Ace1, and ...most ...
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Reduction of Ribosome Level Triggers Flocculation of Fission Yeast Cells
... fission yeast utilize the ribosome level as a trigger to initiate flocculation for control of the sexual mating process? The answer is that yeast sexual reproduction is a cellular response to unhealthy ...
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HIV 2 Protease resistance defined in yeast cells
... in yeast cells induces cell death, as previously shown for the HIV-1 homologue ...Human cells expressing the HIV-1 Protease die via apoptosis [26], however the ...
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Singularity in Polarization: Rewiring Yeast Cells to Make Two Buds
... wild-type cells (Figure 2D). BEM1-GFP-SNC2 rsr1D cells proliferated with a normal cell-cycle profile (Figure 2E) and a doubling time only slightly longer than that of controls (102 min versus 90 ...As ...
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A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cells.
... cdc39) also display meiotic arrest at a point following spindle pole body duplication, but these latter mutants differ in that they proceed farther, undergoing chro- moso[r] ...
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A microfluidic device to acquire high-magnification microphotographs of yeast cells
... chip, cells were synchronized in the M-phase with nocoda- zole treatment, periodically sampled and fixed after release to a normal cell cycle, and then double-stained cell images (DAPI and Rh-ph) in addition to ...
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SENSITIVITY OF MEIOTIC YEAST CELLS TO ULTRAVIOLET LIGHT
... An increase in viability was obtained when irradiated cells were exposed (on the plates) to photoreactivating light immediately after the irradiation, but not when the exposure [r] ...
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Proliferation/Quiescence: When to start? Where to stop? What to stock?
... quiescent cells depend on the cell ’ s needs and are triggered on a “ on demand ” ...fact, cells may stock the unutilized proteins, as it is probably the case with the actin embedded in Actin ...would ...
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Selection of Apoptosis-Deficient Adenovirus E4orf4 Mutants in Saccharomyces cerevisiae
... the yeast 2m origin of replication, a URA3 marker, and ex- pressed E4orf4 from a galactose-inducible ...promoter. Yeast cells harboring this plasmid grew on glucose in which the ga- lactose promoter ...
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A versatile vector system for generating recombinant EGFP tagged proteins in yeast
... on yeast of homo- and heterologous pro- teins fused with the Enhanced Green Fluorescent Protein (EGFP) at the ...The yeast cells harbouring this plasmid emit a feeble emission compared to the ...
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New method for estimating digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro
... brasiliensis yeast cells by PMNs as a function of time of incubation, strain of fungus, and relative proportions of PMNs and fungal cells % of cellsb at incubation time:.. Numbers in par[r] ...
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Improvement of Invertase Synthesis by the Mutant Saccharomyces cerevisiae through UV Mutagenesis
... type yeast cells were grown in YEP glucose and YEP sucrose medium for invertase assay (Fig 2) to compare their invertase synthesis ...wildtype yeast invertase activities in glucose, sucrose was ...
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Preparation of mango (Mangifera indica L ) wine using a new yeast mango peel immobilised biocatalyst system
... by yeast cells entrapped in corn starch gel (Kandylis et ...detached cells in the wine in the present study were lower when compared to those in the wines produced by immobilised sugarcane pieces ...
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Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images
... protocol, yeast cells are stained with methylene blue to indicate whether they are ...viable cells leaving them unstained. However dead cells are unable to keep the methylene blue from ...
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Candida albicans Morphology Dependent Host FGF-2 Response as a Potential Therapeutic Target
... albicans hyphae (and not yeast cells) increase the FGF-2 response in human endothelial cells.. albicans in the hyphal state is required to.[r] ...
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Inactivation of Transcription Factor Gene ACE2 in the Fungal Pathogen Candida glabrata Results in Hypervirulence
... ace2 cells results in an overwhelming, fatal, systemic ...ace2 cells was therefore caused by vascular occlusion initiated at the time of ...HLS122 cells by Northern blot analysis (data not shown), so ...
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Expression and characterization of the trans-activating protein Tax of human T-cell leukemia virus type I in Saccharomyces cerevisiae.
... To test the ability of the Tax protein to trans activate the HTLV-I enhancer in yeast cells, the pX region of HITLV-I, encoding the Tax protein, was cloned under the control of the yeast[r] ...
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Regulation of Salt Tolerance by Torulaspora delbrueckii Calcineurin Target Crz1p
... Again, this is in striking contrast to the situation in S. cerevisiae, where both ions are extruded trough the same calcineurin- regulated Na ⫹ /Li ⫹ ATPase, ENA1 (40). Our results indicate that calcineurin mediates the ...
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Nonstructural Protein 5A of Hepatitis C Virus Inhibits the Function of Karyopherin β3
... in yeast cells ...HCV-infected cells, thus preventing the initiation of antiviral activities of neighboring ...virus-infected cells, the inhibition of the protein secretion apparatus in the ...
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Detection of Antibodies against Paracoccidioides brasiliensis Melanin in In Vitro and In Vivo Studies during Infection
... Several cell wall constituents, including melanins or melanin-like compounds, have been implicated in the pathogenesis of a wide variety of microbial diseases caused by diverse species of pathogenic bacteria, fungi, and ...
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