yeast genome
Screening the yeast genome for new DNA repair genes
... budding yeast, Saccharomyces ...in yeast [1,2], and completion of the (draft) human and yeast genomes now presents us with the exciting possibility of discovering further genes that underpin DNA ...
5
Fitness Landscape of the Fission Yeast Genome
... Genomic DNA was extracted from insertion libraries using phenol/chloroform extraction. All DNA extracted from a li- brary was processed. DNA was sheared to an average size of 200 bp using a Covaris S2 ultrasonicator ...
13
Stability of Large Segmental Duplications in the Yeast Genome
... The study of the intrinsic stability of different dupli- cation structures has been tackled so far in different ways. Pioneer works on genetic recombination have revealed that tandem arrays of small repetitive units are ...
12
A Slowed Cell Cycle Stabilizes the Budding Yeast Genome
... alter genome stability in budding ...the genome is stabi- lized by erv14 in the study of three kinds of instability (chromosome loss, allelic recombinants, and unstable chro- mosomes), in three different ...
18
Genomic Background Predicts the Fate of Duplicated Genes: Evidence From the Yeast Genome
... cerevisiae genome that a duplicate located in a genomic region with a low-recombination rate is likely to evolve faster than a duplicate in an area of high ...
6
Condensin Depletion Causes Genome Decompaction Without Altering the Level of Global Gene Expression in Saccharomyces cerevisiae
... of yeast genome organization is the tethered clustering of centromeres and telomeres in the nu- clear periphery (Rabl conformation) (Gottschling et ...the genome (Figure ...
14
Fisher: a program for the detection of H/ACA snoRNAs using MFE secondary structure prediction and comparative genomics – assessment and update
... Findings: In this report, we provide a brief update of this work, which was aborted after the publication of experimentally-identified snoRNAs [2] identical to candidates we had identified bioinformatically using Fisher. ...
8
Elevated Genome-Wide Instability in Yeast Mutants Lacking RNase H Activity
... find that deletion and duplication events frequently resulted from homologous recombination between nonallelic Ty ele- ments (Table S6). It is difficult to assess the significance of this observation since Ty elements are ...
59
Freedom and Responsibility in Synthetic Genomics: The Synthetic Yeast Project
... The Sc2.0 team consists of scientists from different backgrounds, diverse settings, and many nations who have come together to work on a single project. With team members from such diverse backgrounds, it is essential ...
8
A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae
... In this study, we have demonstrated the application of our Cas9/ gRNA system for successful integration of a 1.2 kb NatMX cassette into the Met15 or Lys2 loci. But, on many occasions, a larger fragment is required to be ...
15
Genetic Diversity in Yeast Assessed With Whole-Genome Oligonucleotide Arrays
... the yeast sequencing project con- firmed that many subtelomeric genes are duplicated within the genome (Yeast Genome Directory ...in yeast, 9 are also located near within 25 kb of the ...
12
Mechanisms of Haploinsufficiency Revealed by Genome-Wide Profiling in Yeast
... the genome; see supplementary Table described (Winzeler et ...A genome-wide cally and each experiment was replicated 6 times per comparison between the heterozygous and homozygous pool across 5 time points ...
12
Epigenetics Theoretical Limits of Synthetic Genomes : the cases of Artificials Caulobacter
... 1079-kb genome based on the genome of Mycoplasma mycoides ...mycoides genome to 531 kb (473 ...Synthetic Yeast Genome Project ...synthetic genome J CVI- syn3A, a robust minimal ...
17
Chromosome Duplication in Saccharomyces cerevisiae
... budding yeast genome; the sites where replication terminates are more stochastic, since termination will occur whenever and wherever two converging replication forks meet each other (or when a fork reaches ...
41
Radioprotective activity of blackcurrant extract evaluated by in vitro micronucleus and gene mutation assays in TK6 human lymphoblastoid cells
... Introduction: Blackcurrant (Ribs nigrum L.) is a classical fruit that has long been used to prepare juice, jam, liqueur, and sometimes medicines in Europe. Previously, we reported a genome defense effect by the ...
7
Cellular Processes and Pathways That Protect Saccharomyces cerevisiae Cells against the Plasma Membrane-Perturbing Compound Chitosan
... chitosan, yeast cells strongly and continuously activate the cell wall integrity pathway ...in yeast cells challenged with acetic acid/acetate at pH ...
9
The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - a yeast of biotechnological interest
... the genome sequencing at CNS-Genoscope, NS designed and supervised the 454 project at the IPK, MC assembled the 454 data and participated to the gap closure, SB mapped the different genome versions, PPG ...
15
Homology between double-stranded RNA and nuclear DNA of yeast.
... Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cervisiae.. Expression of the mitochondrial genome i[r] ...
6
Genome wide analysis of small heat shock proteins involved in yeast ageing
... adenylate cyclase (AC) Cyr1 (Cdc35). The Cyr1 is then stimulated by GTP/GDP binding Ras proteins (Ras1, Ras2) and produces cAMP thereby activating PKA promoting dissociation of the regulatory subunits from the catalytic ...
231
Histone Chaperone Paralogs Have Redundant, Cooperative, and Divergent Functions in Yeast
... The yeast TRAMP5 complex recognizes and polyadeny- lates aberrant RNA transcripts to target them for degrada- tion by the Rrp6 ribonuclease (Schmidt and Butler ...the genome, including those within the rDNA ...
16