Yeast two-hybrid interaction
pISTil: a pipeline for yeast two hybrid Interaction Sequence Tags identification and analysis
... unique host protein partners. Based on these criteria, these protein-protein interactions were alternatively con- firmed by GST pull-down validation with a success rate of 80% [8], underlying the efficiency of the pISTil ...
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Protein Interactions on Telomeric Retrotransposons in Drosophila
... Telomere length in Drosophila is maintained by targeted transposition of three non-LTR retrotransposons: HeT-A, TART and TAHRE (HTT), but understanding the regulation of this process is hindered by our poor knowledge of ...
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A member of the Plasmodium falciparum PHIST family binds to the erythrocyte cytoskeleton component band 4 1
... Yeast two-hybrid interaction validation and mapping In order to recapitulate the interaction observed in the yeast-two-hybrid screen, a fragment of PF3D7_0402000 ...
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Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... Classical Yeast Two-Hybrid System A protein of interest Y is expressed in yeast as a fusion to a DNA- binding domain (DBD, “bait”; circles denote expression ...The two yeast ...
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Functional Replacement of the Intracellular Region of the Notch1 Receptor by Epstein-Barr Virus Nuclear Antigen 2
... 1. Interaction regions of RBP-J with EBNA2 and ...Both yeast two-hybrid assays and coprecipitation experiments using GST-fusion proteins were carried out to measure interaction ...
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Analysis of the interaction of viral RNA replication proteins by using the yeast two-hybrid assay.
... 1a-1a interaction in other tripartite RNA ...1a-1a interaction is a general property for all tripartite RNA viruses, we examined whether the 1a-1a inter- action was observed in closely related tripartite ...
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The Rotavirus Enterotoxin NSP4 Directly Interacts with the Caveolar Structural Protein Caveolin-1
... direct interaction between NSP4 residues 112 to 140 and caveolin-1 was determined by the Pro-Quest yeast two- hybrid system with full-length NSP4 and seven overlapping deletion mutants as ...
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Evaluation of Interactions of Human Cytomegalovirus Immediate-Early IE2 Regulatory Protein with Small Ubiquitin-Like Modifiers and Their Conjugation Enzyme Ubc9
... 1. Interaction of IE2 with SUMO-1, SUMO-2, SUMO-3, and Ubc9 in yeast two-hybrid ...(A) Interaction of GAL4-BD/IE2(87–542) with GAL4-A fusion ...
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Original Article Screening of APP interaction proteins by DUALmembrane yeast two-hybrid system
... the yeast two-hybrid screen was performed and several genes in the Human Brain cDNA Library were obtained whose protein products have interaction with ...this interaction has any ...
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Development of an optimized interaction mating protocol for large scale yeast two hybrid analyses
... in yeast with a library of cDNA fused to the activation domain of Gal4 (the ...productive interaction between the bait fusion protein and an interacting partner protein results in expression of a reporter ...
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YEAST two-hybrid and itc studies of alpha and beta spectrin interaction at the tetramerization site
... the interaction between βII and αII, wild-type or its mutants, at the tetramerization ...GAL4 Two-Hybrid System 3 (Clontech, Mountain View, CA) was ...The yeast strain Y187, which is ...
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Yeast two-hybrid: State of the art
... of yeast promoters. The initiation of gene transcription in yeast, as in other organisms, is achieved by several molecular mechanisms working in ...All yeast genes are preceded by a region containing ...
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The Multimerization of Hantavirus Nucleocapsid Protein Depends on Type-Specific Epitopes
... Recently, two studies have reported on the hantavirus NP-NP interaction; one examined two Ameri- can hantaviruses, SNV and PHV (1), and the other examined the European hantaviruses Tula virus, PUUV, ...
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Orphan nuclear hormone receptor NR4A1 interacts with HPV16 E2 regulatory protein
... The yeast two-hybrid analysis indicated an interaction between orphan nuclear receptor NR4A1 and human papillomavirus type 16 E2 regulatory ...the two-hybrid-dependent activation ...
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In the present A work on selective breeding aimed at further consolidation of key qualities of the race of Zalaajinst Edren white goats towards increased length and fineness of fibers and upgrading phenotypic characteristics (Dorjbat Yo, 2013) resulted i
... the two-hybrid ...The two-hybrid screen is a powerful tool for the characterization of protein-protein interaction using yeast as a model ...the interaction observed ...
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Improved methods for cloning and detection in the yeast two hybrid assay
... represent two cloned in- dependent pGBUD-C2-TRS23 colonies from Fig- ure ...the two plasmids were able to grow on -U-L-H ...and interaction plates (only present -U-L-H + 5 mM3AT plates here) with ...
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A New Method, “Reverse Yeast Two-Hybrid Array” (RYTHA), Identifies Mutants that Dissociate the Physical Interaction Between Elg1 and Slx5
... We carried out a RYTHA screen (Figure 1) to search for genes that affect the interactions between Elg1 and Slx5. Using SGA technology (Tong et al. 2001), we crossed strain MK14562, a derivative of SBY49 carrying the Elg1 ...
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Interaction of calcium and integrin binding protein 1 with integrin α11 and its possible involvement in pulmonary fibrosis
... the two-hybrid analysis presented here shows that this modification is not required for binding to ...the yeast two-hybrid system were corroborated by immunoprecipitation expe- riments ...
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Mutation of Single Hydrophobic Residue I27, L35, F39, L58, L65, L67, or L71 in the N Terminus of VP5 Abolishes Interaction with the Scaffold Protein and Prevents Closure of Herpes Simplex Virus Type 1 Capsid Shells
... To determine the effect of these mutations on virus replica- tion and capsid assembly, all 24 mutations were introduced into the virus genome. We developed a genetic marker rescue/ marker transfer procedure for the easy ...
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The PDZ Proteins PICK1, GRIP and Syntenin bind multiple glutamate receptor subtypes
... used yeast two-hybrid assays to isolate a novel PDZ-mediated interaction between mGluR7a and ...Direct interaction was confirmed by GST-pull-down assays that additionally reveal a ...
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