Yeast-two-hybrid screen 1
Novel binding partners for Prenylated Rab Acceptor 1 identified by a split ubiquitin yeast two hybrid screen
... the screen was completed, individual yeast colo- nies were isolated, inoculated in liquid media, lysed using acid-washed glass beads, and a plasmid mix was purified using Qiagen Miniprep kit spin columns ...
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Identification of sensory-neuron-specific sodium channel Nav1.8 interacting proteins by yeast-two hybrid screen
... a yeast two-hybrid ...of two copies of the annexin II heavy chain, also known as calpactin I, lipocortin II, or p36, and two copies of p i ...
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A Genetic Test for Yeast Two-Hybrid Bait Competency Using RanBPM
... based two-hybrid screening, we reasoned that a protein that interacts with the Gal4-binding domain (Gal4BD) could be used to test the competency of a Gal4BD–bait fusion protein prior to a screen, ...
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Identification of novel ligands of WDR47, using yeast two-hybrid analysis
... the two domains into close proximity to form the transcription ...AH109 yeast strain, whereas the GAL4 DNA-AD were fused to the proteins within the human foetal cDNA library to form the preys ...
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A Multigene Family That Interacts with the Amino Terminus of Plasmodium MSP-1 Identified Using the Yeast Two-Hybrid System
... the two species) that was transcribed from the opposite strand and was unrelated to the ...contain two small inserts of approximately 20 to 25 amino acids relative to the other molecules, whereas PfM- SRP-5 ...
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YEAST two-hybrid and itc studies of alpha and beta spectrin interaction at the tetramerization site
... the yeast two-hybrid system and random mutagenesis to investigate the effects of amino acid mutations on the tetramerization of non- erythroid (brain) spectrin (fodrin) ...to screen for ...
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Development of an optimized interaction mating protocol for large scale yeast two hybrid analyses
... the yeast two-hybrid system, which gives increased mating ...the yeast cells carrying the cDNA ...to screen an Arabidopsis thaliana cDNA library, in spite of bait- dependent variations ...
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Original Article Screening of APP interaction proteins by DUALmembrane yeast two-hybrid system
... teins, screen for its interacting proteins would be of significant ...the yeast two-hybrid screen was performed and several genes in the Human Brain cDNA Library were obtained whose ...
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A Two-Hybrid Screen of the Yeast Proteome for Hsp90 Interactors Uncovers a Novel Hsp90 Chaperone Requirement in the Activity of a Stress-Activated Mitogen-Activated Protein Kinase, Slt2p (Mpk1p)
... genomic screen for two-hybrid interactions strongly rein- forced by the E33A mutation in an Hsp90-BD bait ...The yeast two-hybrid system detects in vivo protein-protein associ- ...
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Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two hybrid system
... from yeast, the prey plasmids in pu- tatively positive hits were isolated using the Easy Yeast Plasmid Isolation Kit ...initial screen was transformed into Y187 strain, followed by mating with ...
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Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome
... single screen. We looked at interactions that we found in one, two, three, or four combinations and then determined the fraction of these subsets that have been confirmed by either literature curated ...
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A user-friendly platform for yeast two-hybrid library screening using next generation sequencing
... seq screen with a particular bait protein to those from a control screen with the ‘empty’ control vectors is essential to discriminate potential true interactors from background from the library, for ...
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Identification of novel protein interactors of the SV40 large T antigen using the yeast two-hybrid system
... the yeast strain during the screen that allows it to grow on galactose, or by the presence of library proteins that can interact with the LexA DNA-binding domain or directly bind and activate the promoters, ...
199
Screening and identification of host proteins interacting with Theileria annulata cysteine proteinase (TaCP) by yeast two hybrid system
... only two host proteins were identified to inter- act with TaCP after a co-transformation experiment of pGBKT7-TaCP with each of the 7 prey plasmids, which indicated this assay had a high false positive ...to ...
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PEG-4000 increased the mating efficiency of yeast-two hybrid screening process using PmF-box1 as bait
... to screen for the Y2H library which was constructed using RNA from plant samples treated with abscisic acid ( ABA ) and polyethylene glycol ( PEG )-8000 and control ...the yeast and cannot autoactivate the ...
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Yeast two-hybrid: State of the art
... the yeast cells ...for yeast cells that are more difficult to make competent for ...(competent) yeast cells known to be good for transformation. Even by using yeast cells known to have worked ...
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Improved methods for cloning and detection in the yeast two hybrid assay
... The results in Figure 4 will demonstrate the benefit of the additional rep- lica-plating to SD-Ura-Leu-His plates helps. run on the agarose gel, the circular plasmids will show bands different from the duplicated linear ...
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Yeast three hybrid screen identifies TgBRADIN/GRA24 as a negative regulator of Toxoplasma gondii bradyzoite differentiation
... Acknowledgments We thank Anne Kelsen and Phoebe Cole for technical assistance, Stephanie Phelps and Drs. Christopher Huston, Mohamed-Ali Hakimi and Matthew Wargo for helpful discussions, and Drs. Douglas Johnson, Aimee ...
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Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... in two hybrid library ...of two or more reporter genes to assay for an interaction has been reported by Fashena et ...classical yeast two hybrid systems relies on the ...
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pISTil: a pipeline for yeast two hybrid Interaction Sequence Tags identification and analysis
... context, yeast two-hybrid system appears as a promising approach to efficiently reconstruct protein interaction networks at the proteome-wide ...
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