Yeast two-hybrid system
The identification of a novel protein interacting with GABAA receptors using the yeast two-hybrid system
... the yeast strains CGI 945, Y190 and Y187, verified as far as the methods within the yeast two-hybrid system permit, that one of these clones C3, interacts with the GABA^ receptor ...
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Identification of novel protein interactors of the SV40 large T antigen using the yeast two-hybrid system
... first two sections I discuss the states of cellular senescence and ...the yeast two-hybrid system, which is the approach that I used to identify novel T-Ag - interacting ...
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A Multigene Family That Interacts with the Amino Terminus of Plasmodium MSP-1 Identified Using the Yeast Two-Hybrid System
... The yeast two- hybrid system has allowed the study of this complex MSP-1 molecule by defining both intra- and intermolecular protein- protein ...
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Characterization of cDNAs from Nicotiana benthamiana that encode proteins which interact with tomato golden mosaic virus AL2 protein in the yeast two-hybrid system
... a yeast two-hybrid system was used to identify host cDNAs encoding proteins that interact with TGMV AL2 ...the yeast two-hybrid system has proven to be a powerful ...
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Geminivirus Movement Protein Interactions with Host Proteins in the Yeast Two-Hybrid System
... Iwabuchi, K., Li, B., Bartel, P. and Fields, S. 1993. Use of the two-hybrid system to identify the domain of p53 involved in oligomeriztion. Oncogene 8: 1693-1696. Kubo, A. and Kondo, N. 1991. ...
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Original Article Screening of APP interaction proteins by DUALmembrane yeast two-hybrid system
... DUALmembrane system, the yeast two-hybrid screen was performed and several genes in the Human Brain cDNA Library were obtained whose protein products have interaction with ...mammalian ...
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Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two hybrid system
... As yeast is a eukaryote cell, proteins encoded by yeast expression system are likely to be similar to their na- tively expressed ...Y2H system can be an effective and accurate method for ...
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Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome
... We systematically tested all N- and C-terminal baits against all N- and C-terminal VZV preys using standard matrix-based Y2H protocols as described previously [21,22]. The quality of interactions was evaluated by ...
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Identification of two novel host proteins interacting with Toxoplasma gondii 14 3 3 protein by yeast two hybrid system
... to detect the Tg14-3-3 protein expression, total proteins of Y2HGold cells were extracted and 187. examined by immunoblotting assay using the Myc-tag (9B11) mouse mAb (Cell Signaling)[r] ...
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Screening and identification of host proteins interacting with Theileria annulata cysteine proteinase (TaCP) by yeast two hybrid system
... only two host proteins were identified to inter- act with TaCP after a co-transformation experiment of pGBKT7-TaCP with each of the 7 prey plasmids, which indicated this assay had a high false positive ...of ...
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The involvement of the ceramide transporter in Alzheimer’s disease Investigating the binding properties of the ceramide transporter in the human brain by optimizing the split- ubiquitin Yeast Two-Hybrid system
... split-ubiquitin Yeast Two-Hybrid (Y2H) technique (7) was used to detect and identify interactions of the MR domain of CERT L with other ...DUALhunter system (8), the goal was to detect protein ...
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A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53
... Mdm2. Two such domains have thus far been reported; the DNA- binding domain of p53 (residues 234–286 within the conserved Boxes IV and V) has been shown to interact with the central acidic domain of Mdm2 [23] and ...
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Yeast two-hybrid: State of the art
... the yeast two-hybrid system are reviewed by ...This yeast strain contains three reporter genes: HIS3, ADE2 and LacZ, each under control of a different promoter (GAL1, GAL2, and GAL7 ...
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Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... first yeast two-hybrid ...their system, protein-protein interactions were tested by fusing one test protein to the DNA-binding domain of the yeast GAL4 transcription factor, and the ...
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pISTil: a pipeline for yeast two hybrid Interaction Sequence Tags identification and analysis
... context, yeast two-hybrid system appears as a promising approach to efficiently reconstruct protein interaction networks at the proteome-wide ...
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YEAST two-hybrid and itc studies of alpha and beta spectrin interaction at the tetramerization site
... the yeast two-hybrid system and random mutagenesis to investigate the effects of amino acid mutations on the tetramerization of non- erythroid (brain) spectrin (fodrin) ...
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Analysis of the interaction of viral RNA replication proteins by using the yeast two-hybrid assay.
... No interactions were observed between the following heter- ologous pairs of proteins: 1a with 3a or CP, CP with 2a-N or 2a D C, and 3a with 2a-N or 2a D C or CP (Table 4 and data not shown). Also, no interactions were ...
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Development of an optimized interaction mating protocol for large scale yeast two hybrid analyses
... Screening a cDNA library with the yeast two-hybrid system is laborious and time consuming. For large-scale and multi- parallel screenings, current protocols are limited, and approaches using ...
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Screening and identification of host proteins interacting with Toxoplasma gondii SAG2 by yeast two hybrid assay
... Yeast two-hybrid system (Y2H) Y2H system was performed between recombinant pGBKT7-SAG2 (bait) and human cDNA human library (prey) (Clontech, ...Y187 yeast by cloning into the ...
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Identification of sensory-neuron-specific sodium channel Nav1.8 interacting proteins by yeast-two hybrid screen
... The yeast two-hybrid screen is one method that can be used to identify interactive ...the yeast two-hybrid system depends on a number of factors, one o f which is the ...
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