ZEB1/2
Original Article MiR-130a regulates the proliferation and metastasis of HCC cells through targeting ZEB1/2
... Abstract: Accumulating evidence indicates that microRNA-130a (miR-130a) function as a tumor suppressor, in- volved in many biological processes including tumor initiation, development and metastasis. However, the ...
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miR-144 functions as a tumor suppressor in breast cancer through inhibiting ZEB1/2-mediated epithelial mesenchymal transition process
... A 2 × power taq polymerase chain reaction (PCR) mastermix (Biotek, Beijing, People’s Republic of China) was used for real time PCR to detect mRNA ...follow: ZEB1, sense, TCCATGCTTA AGAGCGCTAGCT, antisense, ...
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TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition
... We made use of the murine mammary gland cell line NMuMG to further investigate the role of the AP-2 fam- ily members TFAP2A and TFAP2C in EMT. Upon in- duction with TGFβ1, NMuMG cells undergo EMT, which manifests ...
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<p>LncRNA PCAT6 Accelerates the Progression and Chemoresistance of Cervical Cancer Through Up-Regulating ZEB1 by Sponging miR-543</p>
... of ZEB1 attenuates the suppressive impacts of PCAT6 interference on the proliferation, metastasis and chemoresistance and the promoting effect on the apoptosis of CC ...of ZEB1 and the level of PCAT6 was ...
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<p>Modulation of MnSOD and FoxM1 Is Involved in Invasion and EMT Suppression by Isovitexin in Hepatocellular Carcinoma Cells</p>
... Slug, ZEB1 and MMP-2) levels in HCSLCs after treatment with non-cytotoxic concentrations ...Slug, ZEB1 and MMP-2, in a dose-dependent manner (Figures 2E and F; Supplementary Figure ...Slug, ...
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<p>Circular RNA hsa_circ_0023404 promotes proliferation, migration and invasion in non-small cell lung cancer by regulating miR-217/ZEB1 axis</p>
... and ZEB1, while U6 was employed as the loading control for ...and ZEB1 were calculated using the 2 −ΔΔCT ...; ZEB1 forward: 5 ʹ -CCC AGG ACA GCA CAG TAA AT-3 ʹ , reverse: 5 ʹ -GAT GGT GTA CTA ...
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<p>Meg3 Induces EMT and Invasion of Glioma Cells via Autophagy</p>
... cation, 2 −ΔΔCt was calculated and utilized as an indicator of relative expression ...; ZEB1 forward, 5 ′ -ACTCTGATTCTACACCGC -3 ′ ; ZEB1 reverse, 5 ′ -TGTCACATTGATAGGGCTT-3 ′ ; ZEB2 forward, 5 ′ ...
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Involvement of Ras GTPase-activating protein SH3 domain-binding protein 1in the epithelial-to-mesenchymal transition-induced metastasis of breast cancer cells via the Smad signaling pathway
... Metastases in the lungs of mice carrying tumors were confirmed via H&E staining and the lung weight (Figure 7D and 7E, lower). We found many micrometastases in the lungs of mice carrying Mock tumors, in stark ...
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Schmidt, Johanna Maria (2017): Temporal and cell-specific effects of the basic Helix-Loop-Helix Transcription factor Twist1 during breast cancer progression. Dissertation, LMU München: Medizinische Fakultät
... Although ZEB1 expression in EMT resistant cells was not up-regulated as rapid and strong as in EMT competent cells, a significant increase of ZEB1 transcript levels was detectable after long-term ...why ...
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Inhibition of ZEB1-AS1 confers cisplatin sensitivity in breast cancer by promoting microRNA-129-5p-dependent ZEB1 downregulation
... MCF-7 and MDA-MB-231 cells in the logarithmic growth phase were transfected, followed by removal of the cell culture medium after 48 h. Next, the cells were rinsed with pre-cooled PBS 3 times, after which the prepared ...
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Non vesicle bound free microRNAs could enter cells and affect gene expression
... Background: Cell-free microRNAs (miRNAs) exist in body fluid. Previous studies showed that cell-free mi- RNAs are partly bound in microvesicles, and could transfer between cells via fusion with cell membrane. Methods: We ...
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Original Article Long noncoding RNA ZEB1-AS1 predicts an unfavorable prognosis of non-small lung cancer and regulates epithelial to mesenchymal transition through reducing ZEB1 expression
... Four NSCLC adenocarcinoma cell lines (A549, H1975, H1299, and SPCA1), two NSCLC squa- mous carcinomas cell lines (H1703 and SK-MES-1), and a normal human bronchial epi- thelial cell line (16HBE), obtained from the ...
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Either ZEB1 or ZEB2/SIP1 Can Play a Central Role in Regulating the Epstein-Barr Virus Latent-Lytic Switch in a Cell-Type-Specific Manner
... FIG. 2. Both ZEB1 and ZEB2 repress transcription of Zp via binding the ZV ...showing ZEB1 and ZEB2 binding to Zp. ZEB1 and ZEB2, synthesized in a cell-free system (TNT), were incubated with a ...
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ZEB1 drives epithelial to mesenchymal transition in lung cancer
... of ZEB1, ZEB2, and SNAI1. ZEB1 had the most significant correlation with expres- sion of the mesenchymal marker vimentin (VIM) in both our iso- genic series of oncogenically manipulated HBEC3 and a panel of ...
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ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism
... factor ZEB1 is a known driver of EMT, and mediators of ZEB1 represent potential therapeutic targets for metastasis suppres- ...lines, ZEB1 activated PI3K by derepressing miR-200 targets, including ...
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Original Article Association of ZEB1 and FOXO3a protein with invasion/metastasis of non-small cell lung cancer
... A total of 130 pairs of tumor tissues and the adjacent normal tissues (distance >5 cm) of NSCLC patients diagnosed by clinical pathology were collected from June 2013 to June 2015 at the Cangzhou Central Hospital. ...
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miRNA-641 inhibits the proliferation, migration, and invasion and induces apoptosis of cervical cancer cells by directly targeting <em>ZEB1</em>
... anti-human ZEB1 primary antibody (1:1,000 dilution; cat no: ab203829) or rabbit anti-human GAPDH primary antibody (1:1,000 dilu- tion; cat no: ab181603; both from Abcam, Cambridge, MA, ...
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ZEB1-AS1 initiates a miRNA-mediated ceRNA network to facilitate gastric cancer progression
... screening the data from 375 GC and 32 adjacent normal tissue samples (Additional file 4). Using RNA22 (ver- sion 2.0), 777 miRNAs potentially binding to ZEB1-AS1 were identified (Additional file 5). By ...
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Role and prognostic significance of the epithelial-mesenchymal transition factor ZEB2 in ovarian cancer
... Western blots were done on total lysates or on nuclear/cytoplasmic fractions. Total cellular proteins were obtained lysing the cells with EB buffer [20 mmol/L Tris-HCl (pH 7.4), 5 mmol/L EDTA, 150 mmol/L NaCl, 10% ...
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MYC associated zinc finger protein promotes the invasion and metastasis of hepatocellular carcinoma by inducing epithelial mesenchymal transition
... and 2 (ZEB1 and ZEB2), two important mesenchymal markers in epithelial- mesenchymal transition (EMT) that were over-expressed in ...besides ZEB1 and ZEB2 by decreasing MAZ expression, both detected ...
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