[PDF] Top 20 Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells
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Adaptation of Subtype A Human Immunodeficiency Virus Type 1 Envelope to Pig-Tailed Macaque Cells
... on human PBMCs, Ptm PBMCs, and immortalized Ptm lymphocytes. Live cells were gated on CD4 expression, and CD4-positive cells are shown in the ...unstained cells and, in the PBMC expression ... See full document
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Novel Pathway for Induction of Latent Virus from Resting CD4+ T Cells in the Simian Immunodeficiency Virus/Macaque Model of Human Immunodeficiency Virus Type 1 Latency
... activate macaque resting CD4 ⫹ T cells and induce latent SIV from these ...resting macaque CD4 ⫹ T cells with the human B-T-hybrid cell line CEMx174 or with EBV-transformed human ... See full document
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Experimental Infection of Rhesus and Pig-Tailed Macaques with Macaque Rhadinoviruses
... in macaque monkeys has spurred interest in their use as models for human infection with Kaposi sarcoma-associated herpesvirus (human herpesvirus ...and pig-tailed macaques (Macaca ... See full document
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Simian-Human Immunodeficiency Virus Containing a Human Immunodeficiency Virus Type 1 Subtype-E Envelope Gene: Persistent Infection, CD4+ T-Cell Depletion, and Mucosal Membrane Transmission in Macaques
... measure virus load in lymph node cells, peripheral lymph node samples were obtained by transcutaneous biopsy and aseptically teased into single-cell suspensions; cell numbers were determined by counting in ... See full document
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Comparative Immunogenicity of Subtype A Human Immunodeficiency Virus Type 1 Envelope Exhibiting Differential Exposure of Conserved Neutralization Epitopes
... Expression and purification of soluble gp140. The gp140 versions of Envs used as immunogens were expressed transiently by bulk transfecting HEK293 cells at 4L scale. The media were collected 72 to 96 h after ... See full document
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Evaluation of Envelope Vaccines Derived from the South African Subtype C Human Immunodeficiency Virus Type 1 TV1 Strain
... 293T cells were transfected with 2 g of gp160-expression vectors using FuGENE-6 (Roche Molecular Biochemicals) in 100-mm culture ...dishes. Envelope-expressing 293T cells were replated at 6 h ... See full document
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Efficacy of Postexposure Prophylaxis after Intravaginal Exposure of Pig-Tailed Macaques to a Human-Derived Retrovirus (Human Immunodeficiency Virus Type 2)
... (Table 1; ...isolate virus from PBMC (data not shown), and a classic antibody response beginning at 3 or 4 weeks ...plasma virus became undetectable by week 12 to 16 p.i. Interestingly, ... See full document
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A Reliable Phenotype Predictor for Human Immunodeficiency Virus Type 1 Subtype C Based on Envelope V3 Sequences
... establishing human immunodeficiency virus type 1 (HIV-1) infection generally use CCR5 as a coreceptor for entry into host cells (11, ...the virus to use other ... See full document
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Emergence of gp120 V3 Variants Confers Neutralization Resistance in an R5 Simian-Human Immunodeficiency Virus-Infected Macaque Elite Neutralizer That Targets the N332 Glycan of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein
... 169 cells/ l and continued to fall between weeks 86 and 100 ...PS virus (from 1:5,193 [week 77] to 1:397 [week 101]) (20), with both parameters ostensibly reflecting a deteriorating and ... See full document
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Human Immunodeficiency Virus Type 1 Subtype B Ancestral Envelope Protein Is Functional and Elicits Neutralizing Antibodies in Rabbits Similar to Those Elicited by a Circulating Subtype B Envelope
... Human immunodeficiency virus type 1 (HIV-1) is a difficult target for vaccine development, in part because of its ever-expanding genetic diversity and attendant capacity to ... See full document
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Cross-Subtype Neutralizing Antibodies Induced in Baboons by a Subtype E gp120 Immunogen Based on an R5 Primary Human Immunodeficiency Virus Type 1 Envelope
... from subtype B (IIIB and SF2) and subtype E (NPO3, 9466, 9461, and 42368) were harvested from acutely infected H9 ...and subtype E (9466, CM235, CMU06, and 8868) isolates were isolated from PBMC of ... See full document
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Adaptation of the Human Immunodeficiency Virus Type 1 Envelope Glycoproteins to New World Monkey Receptors
... the cells of most New World monkeys. The principal early block to HIV-1 infection in New World monkey cells occurs at the level of virus entry (19, 30, ...(TM) envelope glycoproteins, ... See full document
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Cross-Subtype Neutralization Sensitivity despite Monoclonal Antibody Resistance among Early Subtype A, C, and D Envelope Variants of Human Immunodeficiency Virus Type 1
... Table 1; the timing of first infection was determined by both HIV-1 serology and HIV RNA testing as described previously ...293T cells along with an env-deficient HIV-1 subtype A ... See full document
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Genotyping of TRIM5 locus in northern pig tailed macaques (Macaca leonina), a primate species susceptible to Human Immunodeficiency Virus type 1 infection
... host cells [7-9]. It has been doc- umented that in Old World monkey cells, CypA is required for TRIM5α-mediated resistance to HIV-1 ...simian immunodeficiency virus (SIV) and feline ... See full document
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Chimeric simian/human immunodeficiency virus that causes progressive loss of CD4+ T cells and AIDS in pig-tailed macaques.
... CD4 1 T ...CD4 1 T-cell loss correlated with increased virus ...The virus appeared more virulent in pas- sage 4. The CD4 1 T-cell count in one of the two macaques, PNb, fell rapidly ... See full document
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Autologous Neutralizing Humoral Immunity and Evolution of the Viral Envelope in the Course of Subtype B Human Immunodeficiency Virus Type 1 Infection
... cells. This may be true in particular for V3, known as a con- served neutralizing domain (19), as four out of six mutations in gp120 observed in viruses from three patients (or in four pa- tients for mutation ... See full document
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A Variant Macaque-Tropic Human Immunodeficiency Virus Type 1 Is Resistant to Alpha Interferon-Induced Restriction in Pig-Tailed Macaque CD4+ T Cells
... the virus (Yu-envL) showed a level of resistance to IFN- ␣ that was similar to that of the HSIV-vif-Yu2 parent virus ...enhanced virus production in IFN- ␣ -treated costimulated human PBMCs, ... See full document
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Enhanced Infectivity of an R5-Tropic Simian/Human Immunodeficiency Virus Carrying Human Immunodeficiency Virus Type 1 Subtype C Envelope after Serial Passages in Pig-Tailed Macaques (Macaca nemestrina)
... of 1⫻ fluorescence-activated cell sorter (FACS) lysing solution (Becton Dickinson) was added, and another incubation at room tem- perature for 15 min was carried out to ensure the total lysis of red blood ... See full document
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Infectious Simian/Human Immunodeficiency Virus with Human Immunodeficiency Virus Type 1 Subtype C from an African Isolate: Rhesus Macaque Model
... the envelope in SHIV MJ4 used only CCR5 and not CCR1, CCR2(b), CCR3, or CXCR4 as a coreceptor for cell entry (36, ...HIV-1 envelope could be clearly detected by 6 weeks post inoculation in the 2 ... See full document
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Evolution of a Human Immunodeficiency Virus Type 1 Variant with Enhanced Replication in Pig-Tailed Macaque Cells by DNA Shuffling
... a human immunodeficiency virus type 1 (HIV-1) variant with enhanced replication in pig-tailed macaque peripheral blood mononuclear cells (pt ...simian ... See full document
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