[PDF] Top 20 Amplified Fragment Length Polymorphism Analysis: the State of an Art
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Amplified Fragment Length Polymorphism Analysis: the State of an Art
... morphisms analysis, which is a PCR between an adapter se- quence and a labeled primer specific for a plant retrotranspo- son (27, 60, 119) or the IS6110 element of Mycobacteria tuberculosis (34), is used to ... See full document
9
Differentiation of Burkholderia Species by PCR Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene and Application to Cystic Fibrosis Isolates
... accurate identification of B. cepacia with widely used multitest commercial systems has been recently shown to be problematic (12). Second, the genus Burkholderia has undergone multiple changes since its ... See full document
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Amplified Fragment Length Polymorphism Analysis versus Macro Restriction Fragment Analysis for Molecular Typing ofStreptococcus pneumoniae Isolates
... times with 75% (vol/vol) ethanol, dried, and redissolved in 100 ml of TE buffer (10 mM Tris-HCl, 1 mM EDTA [pH 7.6]) with 25 ml DNase-free RNase (0.25 mg/ml) for 1.5 h at 37°C. Prep- aration of AFLP templates was ... See full document
5
Analysis of Two Clonal Lines (Embryogenic and Non Embryogenic) of Agave fourcroydes Using AFLP and MSAP
... comparative analysis of the genetic differences through amplified fragment length polymorphism (AFLP) and the epigenetic differences through methylation-sensitive amplified ... See full document
18
Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR Based Restriction Fragment Length Polymorphism Analysis
... A molecular typing approach for Campylobacter jejuni and Campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb PCR-amplified portion of the ... See full document
7
Genome Sequence Based Fluorescent Amplified Fragment Length Polymorphism Analysis ofMycobacterium tuberculosis
... fluorescent amplified-fragment length polymorphism (FAFLP) analysis, was applied to Mycobacterium ...FAFLP analysis was broadly congruent with IS6110 typing for those strains ... See full document
6
Genotyping of Epidemic Methicillin ResistantStaphylococcus aureus Phage Type 15 Isolates by Fluorescent Amplified Fragment Length Polymorphism Analysis
... fluorescent amplified-fragment length polymorphism (FAFLP) analysis requires double digestion of the bacterial genome with restriction endonucleases, followed by ligation of adaptor ... See full document
6
Fluorescent Amplified Fragment Length Polymorphism Analysis of an Outbreak of Group A Streptococcal Invasive Disease
... Bacterial strains and culture conditions. Twenty-six isolates of S. pyogenes from patients with invasive or noninvasive streptococcal diseases were obtained from the Streptococcus and Diphtheria Reference Unit, ... See full document
5
Epidemiology of Human Sporotrichosis Investigated by Amplified Fragment Length Polymorphism
... The relationship between the genetic diversity and ecology of S. schenckii has not been studied. S. schenckii has been isolated from a variety of environmental sources. Also, sporo- trichosis occurs in cats, dogs, and ... See full document
5
DNA Variation in the Wild Plant Arabidopsis thaliana Revealed by Amplified Fragment Length Polymorphism Analysis
... To investigate the level and pattern of DNA variation of Arabidopsis thaliana at the entire genome level, AFLP analysis was conducted for 38 ecotypes distributed throughout the world. Ten pairs of selective ... See full document
10
Use of Fluorescent Amplified Fragment Length Polymorphism for Molecular Epidemiology of Leptospirosis in India
... Selective FAFLP PCR with six sets of primers generated a total of 76 polymorphic fragments ranging in size from 50 to 500 bp (Fig. 1). Analysis of a phylogenic tree constructed using the neighbor-joining method ... See full document
6
Population structure of South African and Australian Pyrenophora teres isolates
... study, amplified fragment length polymorphism analysis was used to investigate the genetic diversity and population structure of 60 PTT and 64 PTM isolates collected across Australia ... See full document
36
Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... In conclusion, the present study demonstrates the limita- tions of the 16S rRNA-based PRPA technique to differentiate M. ulcerans from M. marinum and the usefulness of the DNA fingerprinting techniques utilizing IS2404 ... See full document
7
Distinguishing among Magnolia cultivars using fluorescent Amplified Fragment Length Polymorphism (AFLP) analysis
... Table 2 Polymorphism detected with four amplified fragment length polymorphism (AFLP) primer combinations for 13 Magnolia samples. AFLP Primer combination No.of No.[r] ... See full document
20
Identification of Clostridium Species and DNA Fingerprinting of Clostridium perfringens by Amplified Fragment Length Polymorphism Analysis
... C. novyi type A and C. botulinum types C and D cannot be differentiated from each other by their phenotypic properties, and C. novyi type A is considered a nontoxigenic variant of group III C. botulinum (14). That group ... See full document
9
Recognition of SCCmec Types According to Typing Pattern Determined by Multienzyme Multiplex PCR Amplified Fragment Length Polymorphism Analysis of Methicillin Resistant Staphylococcus aureus
... PFGE is the gold standard in typing of S. aureus, and a web-based database has been recently set up to compare in- tercenter typing patterns that allow tracing of isolates (10). However, for typing of large strain ... See full document
6
Amplified fragment length polymorphism typing of HLA-DPB1 and associations with IDDM and MS
... Analysis of the amino acid sequence variation of the al and a2 domains in the context of the three dimensional structure of the antigen binding site has shown that most polymorphic amino[r] ... See full document
254
Exploration of amplified fragment length polymorphism analysis as an effective tool for discriminating pathogenic strains of bacteria
... In order for an assay to be useful in a clinical or forensic testing capacity, it must be validated. In this context, valida- tion incorporates a deep understanding of the performance characteristics of the assay with ... See full document
9
Genotyping Neisseria gonorrhoeaeUsing Fluorescent Amplified Fragment Length Polymorphism Analysis
... FIG. 3. FAFLP-generated distance tree for 52 strains of N. gonorrhoeae. FAFLP was carried out in duplicate for all strains. These gave identical profiles except for strain 12589, which differed by one fragment ... See full document
5
Amplified Fragment Length Polymorphism Fingerprinting of Mycoplasma Species
... isoenzyme analysis (32), one- and two- dimensional gel electrophoresis (24, 30), and immunoblotting (4) possess the required discriminatory power but are time- consuming, require large samples for analysis, ... See full document
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