[PDF] Top 20 Analysis of the Association of the Human Cytomegalovirus DNA Polymerase Subunit UL44 with the Viral DNA Replication Factor UL84
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Analysis of the Association of the Human Cytomegalovirus DNA Polymerase Subunit UL44 with the Viral DNA Replication Factor UL84
... with UL44 from HCMV-infected ...with UL44 during HCMV infection, mock- infected and infected cell lysates were prepared 72 h postin- fection and IP was performed using an MAb recognizing ...UL44. ... See full document
9
Dynamic and Nucleolin-Dependent Localization of Human Cytomegalovirus UL84 to the Periphery of Viral Replication Compartments and Nucleoli
... of viral and cellular proteins with ...the association of UL44, UL84FLAG, and nucleolin in infected cells, we performed coim- munoprecipitation assays ...of UL44 [FLAGUL44]), or AD169-UL84FLAG ... See full document
10
Multiple regulatory events influence human cytomegalovirus DNA polymerase (UL54) expression during viral infection.
... The human cytomegalovirus (HCMV) DNA polymerase gene (UL54; also called pol) is a prototypical early gene in that expression is mandatory for viral DNA ...to viral ... See full document
10
The Pre-NH2-Terminal Domain of the Herpes Simplex Virus 1 DNA Polymerase Catalytic Subunit Is Required for Efficient Viral Replication
... a factor that actively recruits the polymerase to the replication fork or other- wise positively impacts viral DNA ...the viral helicase-primase complex, has been shown to ... See full document
9
The Flexible Loop of the Human Cytomegalovirus DNA Polymerase Processivity Factor ppUL44 Is Required for Efficient DNA Binding and Replication in Cells
... of UL44-FL resulted in an increase in ppUL44 intranuclear mobility ...quantitative analysis indicates that ca. 50% of GFP-UL44 is retained in the nucleus after deter- gent permeabilization of HEK 293 ... See full document
10
Role of Homodimerization of Human Cytomegalovirus DNA Polymerase Accessory Protein UL44 in Origin-Dependent DNA Replication in Cells
... that UL44 dimerization is a prerequisite for supporting DNA replication in cells, as mu- tations that specifically affect the dimerization of UL44 also impair its ability to complement ... See full document
6
The Late Promoter of the Human Cytomegalovirus Viral DNA Polymerase Processivity Factor Has an Impact on Delayed Early and Late Viral Gene Products but Not on Viral DNA Synthesis
... PCR analysis using the UL99 primers and probe detects the total amount of the UL92 to -99 ...blot analysis using the UL99 probe showed that in the presence of PAA, the ...(42), analysis of ... See full document
10
Evidence that the UL84 gene product of human cytomegalovirus is essential for promoting oriLyt-dependent DNA replication and formation of replication compartments in cotransfection assays.
... HCMV replication compartments actively synthesize DNA and incorporate ...the replication compartments visualized in individual cells cotransfected with HCMV core, auxiliary, and origin components ... See full document
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Role of the Specific Interaction of UL112-113 p84 with UL44 DNA Polymerase Processivity Factor in Promoting DNA Replication of Human Cytomegalovirus
... of UL44. A structural analysis using the amino-terminal 290 amino acids of UL44, which corresponds to UL44( ⌬ C290) in this study, showed that UL44 has a structural fold remarkably ... See full document
13
The Carboxy-Terminal Segment of the Human Cytomegalovirus DNA Polymerase Accessory Subunit UL44 Is Crucial for Viral Replication
... of UL44 C-terminal truncations in various ...oriLyt DNA replication origin), a plasmid expressing WT or mutant UL44 (as indicated at the top of the panel), and plasmids expressing all of the ... See full document
6
Nucleolin Associates with the Human Cytomegalovirus DNA Polymerase Accessory Subunit UL44 and Is Necessary for Efficient Viral Replication
... potential association of UL44 and nucleolin in infected cell ...a DNA and RNA binding phosphoprotein that interacts with multiple cel- lular proteins and appears to have multiple functions in ribo- ... See full document
14
Recruitment of Brd4 to the Human Papillomavirus Type 16 DNA Replication Complex Is Essential for Replication of Viral DNA
... the viral Ori and recruits cellular replication factors to the viral DNA replication complex ...the viral proteins colocalize as tiny dots ... See full document
14
Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA
... Primary human foreskin fibroblast (HFF) cells and human embryonic lung (HEL) cells were grown in mon- olayer cultures in Dulbecco's modified Eagle medium (Gibco BRL, Gaithersberg, MD) supplemented with 100 ... See full document
14
Genomic localization, sequence analysis, and transcription of the putative human cytomegalovirus DNA polymerase gene.
... In the course of sequencing the entire HCMV genome, a 3.7-kb open reading frame within the EcoRI M fragment has been identified that can be aligned to the HSV-1 and putative EB3V polymer[r] ... See full document
6
Human Cytomegalovirus UL84 Localizes to the Cell Nucleus via a Nuclear Localization Signal and Is a Component of Viral Replication Compartments
... for UL84. Transient transfections clearly demonstrated that UL84 localized to the nucleus in the absence of any other viral pro- ...of UL84 expression constructs where increas- ing amounts of ... See full document
8
Human Cytomegalovirus Can Procure Deoxyribonucleotides for Viral DNA Replication in the Absence of Retinoblastoma Protein Phosphorylation
... noted. Viral and cellular DNAs were extracted with an IBI DNA isolation kit ...(IB47202). DNA levels were quantitated by real- time PCR (ABI 7900HT), using primers and a TaqMan probe specific for IE1 ... See full document
10
A Mutation Deleting Sequences Encoding the Amino Terminus of Human Cytomegalovirus UL84 Impairs Interaction with UL44 and Capsid Localization
... Mislocalization of viral capsids in BADGFPUL84⌬68-in- fected cells. Based on the above results, we examined capsid produc- tion and localization in cells infected with our mutant virus. Cells infected with either ... See full document
13
Autographa californica Multiple Nucleopolyhedrovirus DNA Polymerase C Terminus Is Required for Nuclear Localization and Viral DNA Replication
... The DNA polymerase (DNApol) of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is essen- tial for viral DNA ...and polymerase domains are highly conserved ... See full document
16
DNA polymerase requirements for parvovirus H-1 DNA replication in vitro.
... We have recently demonstrated the ability of purified polymerase -y to carry out the synthesis in vitro of RF from single-stranded viral DNA 1Sa, a reaction quite similar or identical to[r] ... See full document
8
The UL84 protein of human cytomegalovirus acts as a transdominant inhibitor of immediate-early-mediated transactivation that is able to prevent viral replication.
... HCMV replication after transient expression of ...prevent viral replication when expressed aberrantly during ini- tial stages of the viral replicative cycle, we first performed ... See full document
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