[PDF] Top 20 Analysis of the Interaction between the Essential Herpes Simplex Virus 1 Tegument Proteins VP16 and VP1/2
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Analysis of the Interaction between the Essential Herpes Simplex Virus 1 Tegument Proteins VP16 and VP1/2
... is essential for VP16 to bind VP1/2 was surprising as, although this residue is highly conserved, the re- solved crystal structure of the soluble VP16 core domain predicts that K343 is ... See full document
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The Major Determinant for Addition of Tegument Protein pUL48 (VP16) to Capsids in Herpes Simplex Virus Type 1 Is the Presence of the Major Tegument Protein pUL36 (VP1/2)
... study, analysis of the com- position of C capsids purified from cells infected with HSV-1 constructs lacking the gene for either pUL35, pUL36, or pUL37 illustrated for the first time that pUL48 requires the ... See full document
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Nuclear Localizations of the Herpes Simplex Virus Type 1 Tegument Proteins VP13/14, vhs, and VP16 Precede VP22-Dependent Microtubule Reorganization and VP22 Nuclear Import
... panels 1 to 3). In contrast, in HSV-1(F)-infected cells, MTOCs were only detected at 5 hpi ...HSV- 1(F) or mock infected, fixed at the indicated times, and double stained with anti- ␣ -tubulin ... See full document
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Packaging Determinants in the UL11 Tegument Protein of Herpes Simplex Virus Type 1
... Analysis of UL11 packaging. To compare the relative levels of packaging for wt and mutant derivatives of UL11, a transfection/infection-based assay was developed. Semiconfluent monolayers of Vero cells were ... See full document
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The Capsid and Tegument of the Alphaherpesviruses Are Linked by an Interaction between the UL25 and VP1/2 Proteins
... acquire tegument proteins remains a key question in viral ...pseudorabies virus (PRV), we have previously shown that the 62 carboxy-terminal amino acids of the VP1/2 large ... See full document
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Membrane Association of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
... ument proteins of herpesviruses could collectively be consid- ered both structurally and functionally equivalent to the matrix proteins of RNA ...certain tegument proteins may contain all of ... See full document
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Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16
... UL14-defective virus at later time points after infection, including spherically shaped cells, shrink- age of the cytoplasmic volume, and an inward curvature of the nucleus (Figure 3E, F, K, ...UL14(3P)-VP16GFP ... See full document
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The UL14 Tegument Protein of Herpes Simplex Virus Type 1 Is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids
... actin. VP16-GFP (95 kDa) was detected both by anti-VP16 and anti-GFP antibodies on separate ...of VP16-GFP are present in cell-associated preparations of 14R-VP16G and 14D-VP16G ...cell-associated ... See full document
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Recognition of Herpes Simplex Virus Type 2 Tegument Proteins by CD4 T Cells Infiltrating Human Genital Herpes Lesions
... HLA typing was performed by John Hansen and Effie Petersdorf. HSV-1 3 HSV-2 IRV were graciously provided by Bernard Roizman (RS1G31), Howard Marsden (DX32), and Steve Triezenberg (RP-2). The ... See full document
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Herpes Simplex Virus Transactivator VP16 Discriminates between HCF-1 and a Novel Family Member, HCF-2
... (residues 2 to 373) or complete open reading frame (ORF; residues 2 to 792) were generated by amplification using high-fidelity PCR (Expand high-fidelity PCR system; Boehringer ...introduced between ... See full document
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The Large Tegument Protein pUL36 Is Essential for Formation of the Capsid Vertex-Specific Component at the Capsid-Tegument Interface of Herpes Simplex Virus 1
... apparent between them other than at the capsid vertices, which are known to be sites of interaction between capsid and tegument ...space between the P-hexons that surround the pen- ton ... See full document
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Autocatalytic Activity of the Ubiquitin-Specific Protease Domain of Herpes Simplex Virus 1 VP1-2
... of VP1-2, and isolated polypeptides almost identical to regions and sub- regions of the variants used here have been previously shown to have relevant and specific interaction with virus ... See full document
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Identification of a Highly Conserved, Functional Nuclear Localization Signal within the N-Terminal Region of Herpes Simplex Virus Type 1 VP1-2 Tegument Protein
... region 1 (panel IV), region 2 (panel V), region 3 (panel VII), and region 4 (panel ...alphaherpesvirus VP1-2 pro- teins, it was lacking in the members of the beta- and gammaher- ...in ... See full document
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Herpes simplex virus 1 infection dampens the immediate early antiviral innate immunity signaling from peroxisomes by tegument protein VP16
... HSV-1 proteins was involved in inhibiting MAVS-Pex mediated early induction of ...the tegument protein VP16 was found to most effectively inhibit MAVS-Pex induced viperin mRNA expression ... See full document
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The Unusual Fold of Herpes Simplex Virus 1 UL21, a Multifunctional Tegument Protein
... (Fig. 2). Twelve of these are surface exposed (Fig. 2 and 3A) but do not form obvious clus- ters, which prevented clear assignment of potentially important sites on the surface of UL21N based on sequence ... See full document
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Tsg101 Interacts with Herpes Simplex Virus 1 VP1/2 and Is a Substrate of VP1/2 Ubiquitin-Specific Protease Domain Activity
... large tegument protein a cysteine pro- tease module that displays deubiquitinating (DUB) activity, named herpesvirus tegument ubiquitin-specific protease (htUSP) ...of herpes simplex ... See full document
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Herpes Simplex Virus Tegument Protein VP22 Contains an Internal VP16 Interaction Domain and a C-Terminal Domain That Are Both Required for VP22 Assembly into the Virus Particle
... GFP). VP16 was also present in all the infected cell extracts at similar levels (data not shown) and was coimmunoprecipitated efficiently with full- length VP22 ...D, 1-301). By contrast, precipi- tation of ... See full document
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Analysis of HCF, the Cellular Cofactor of VP16, in Herpes Simplex Virus-Infected Cells
... retain VP16 in the nucleus ...for VP16 nuclear compartmentalization late in ...HCF interaction is involved in late VP16 com- partmentalization but that the interaction is not detected ... See full document
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Protein interactions in the herpes simplex virus type 1 VP16-induced complex: VP16 peptide inhibition and mutational analysis of host cell factor requirements.
... The herpes simplex virus VP16 protein functions as a potent transcriptional activator and targets DNA sites with the consensus TAATGARAT present in all the viral immediate-early gene ...so, ... See full document
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Analysis of the Interaction between the UL11 and UL16 Tegument Proteins of Herpes Simplex Virus
... the interaction with purified UL11, a finding that was further supported by the inability of some (but not all) UL16 cysteine substitution mu- tants to bind ...the interaction of UL16 with the capsid may be ... See full document
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