[PDF] Top 20 Analysis of Prototype Foamy Virus particle host cell interaction with autofluorescent retroviral particles
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Analysis of Prototype Foamy Virus particle host cell interaction with autofluorescent retroviral particles
... N-terminal autofluorescent protein (AFP) fusion, despite apparently normal intracellular capsid ...on particle assembly, egress and particle ...vector particles was reduced up to 100-fold in ... See full document
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Subviral Particle Release Determinants of Prototype Foamy Virus
... major interaction domain for the viral capsid, which is required dur- ing the Env-dependent particle release process, and posttrans- lational modification by ubiquitin, which influences the level of SVP ... See full document
12
Foamy Virus Particle Formation
... classical retroviral replication strategy in several ...of virus particle ...the interaction of the precursor proteins with the cytoplasmic membrane and promote the re- lease of viral ... See full document
6
Analysis and Function of Prototype Foamy Virus Envelope N Glycosylation
... biochemical analysis of the PFV Env mutants, the individual expression constructs were cotransfected together with FV vector pMH118 into 293T cells by the calcium phosphate or polyethylene imine method essentially ... See full document
9
Characterization of Prototype Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Particle Egress and Infectivity
... viral particles were harvested as described previously (22) at 48 h posttransfection for analysis of L-domain mutations or 24 h posttransfection upon cotransfection of expression constructs for DN mutants ... See full document
11
Prototype Foamy Virus Protease Activity Is Essential for Intraparticle Reverse Transcription Initiation but Not Absolutely Required for Uncoating upon Host Cell Entry
... FV particles lacking any viral protease activity was the most strik- ing and unexpected discovery of this ...PR-deficient particles were only ...retrovirus particle which can be assembled from its ... See full document
14
Novel Functions of Prototype Foamy Virus Gag Glycine- Arginine-Rich Boxes in Reverse Transcription and Particle Morphogenesis
... Our results support the current notion of GRII being re- sponsible for PFV Gag nuclear localization because only GRII mutants displayed an altered intracellular distribution. Inter- estingly, Gag subcellular localization ... See full document
12
Characterization of the Prototype Foamy Virus Envelope Glycoprotein Receptor-Binding Domain
... The foamy virus (FV) glycoprotein precursor gp130 Env undergoes a highly unusual biosynthesis, resulting in the generation of three particle-associated, mature subunits, leader peptide (LP), surface ... See full document
10
An N terminal domain helical motif of Prototype Foamy Virus Gag with dual functions essential for particle egress and viral infectivity
... Env interaction by using a pull-down assay in combination with particle release studies and single cycle replication assays using recombinant PFV vector ...The analysis demonstrates a specific direct ... See full document
14
Identification of Host Proteins Associated with Retroviral Vector Particles by Proteomic Analysis of Highly Purified Vector Preparations
... the cell, as do HIV-1 macrophage-derived virions (36, ...of host cell-derived proteins incorporated into retrovirus particles has proved to be difficult due to the presence of contaminating ... See full document
11
Orthoretroviral like prototype foamy virus gag pol expression is compatible with viral replication
... Several Prototype FV (PFV) Gag-Pol fusion protein constructs were generated to examine whether PFV replication is compatible with an orthoretroviral-like Pol ...their analysis, ... See full document
14
Host restriction factors in retroviral infection: promises in virus host interaction
... inhibitory host factors to complete their life ...the virus enter the cell, duplicate its viral genome, make viral proteins, and release new progeny particles, while inhibitory factors could, ... See full document
28
Ubiquitination of the Prototype Foamy Virus Envelope Glycoprotein Leader Peptide Regulates Subviral Particle Release
... subviral particles in the subgenomic and proviral ...efficient interaction of PFV capsids with the glycoprotein and thereby enhancing the glycoprotein’s intrinsic capacity to form subviral ...viral ... See full document
10
The prototype foamy virus protease is active independently of the integrase domain
... These experiments show that the expression of a GagPol fusion protein leads to infectious FVs and a separate Pol is not required in the vector context. The Gag and Pol expression and processing of the GagPol precursor ... See full document
8
Insights into the structure and activity of prototype foamy virus RNase H
... Results: RNase H activity assays demonstrate that the PFV RNase H domain is active, although its activity is about 200-fold reduced as compared to the full length protease-reverse transcriptase enzyme. Fluorescence ... See full document
11
Lassa Virus Cell Entry Reveals New Aspects of Virus-Host Cell Interaction
... 5. Nunberg JH, York J. 2012. The curious case of arenavirus entry, and its inhibition. Viruses 4:83–101. https://doi.org/10.3390/v4010083. 6. Andersen KG, Shapiro BJ, Matranga CB, Sealfon R, Lin AE, Moses LM, Folarin OA, ... See full document
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Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases
... qualitative analysis of DNA polymerization was per- formed by using a heteropolymeric single stranded M13 DNA as a template together with a radioactively 5 ’ end labeled primer and saturating dNTP concentrations ... See full document
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Foamy Virus Envelope Glycoprotein Is Sufficient for Particle Budding and Release
... from cell culture supernatants and visualized by ...extracellular particles were collected, concentrated, and partially purified through an Optiprep ...from cell cul- ture supernatants and that they ... See full document
11
Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
... FV particles [4]? Firstly, with respect to this study only the minimal RNA sequence requirements for Pol protein encapsidation using subge- nomic constructs have been determined, and not the rela- tive ratios ... See full document
7
RNA and Protein Requirements for Incorporation of the Pol Protein into Foamy Virus Particles
... The actual mechanism of FV Pol protein particle incorpo- ration despite lack of a Gag-Pol precursor has remained ob- scure. It is evident that FVs must have found a means of Pol encapsidation that acknowledges ... See full document
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