[PDF] Top 20 Analysis of a putative crtW gene of Myxococcus xanthus
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Analysis of a putative crtW gene of Myxococcus xanthus
... Following a number of ligation reaction alterations, principally involving changing the temperature at which the reaction was carried out from 4 o C to room temperature, amongst other things, a number of potential ... See full document
221
Whole transcriptome analysis and gene deletion to understand the chloramphenicol resistance mechanism and develop a screening method for homologous recombination in Myxococcus xanthus
... To understand why Cm_8h has a long lag growth phase and to elucidate the molecular activity of bacteria at this stage, we investigated upregulated genes in Cm_8h vs NDK, where the number of downregulated genes (1295 ... See full document
15
CrdS and CrdA Comprise a Two Component System That Is Cooperatively Regulated by the Che3 Chemosensory System in Myxococcus xanthus
... developmental gene expression ...vivo analysis and previous results suggesting that Che3 negatively regulates CrdA during development ...M. xanthus FrzE (CheA1) is active only when both CheW and MCP ... See full document
9
Further genetic studies of the carQRS region of Myxococcus xanthus
... The chromosomal ON A of five of these strains (the exception was MR466) were subjected to Southern blot analysis in order to confirm the expected sizes of each of the carQRS regions. This was an important ... See full document
236
Bergeler, Silke (2018): Positioning mechanisms in bacterial cells: midcell sensing in Myxococcus xanthus. Dissertation, LMU München: Fakultät für Physik
... first analysis of this asymmetry in the biological system indeed showed a slightly, but significantly asymmetric PomZ distribution on the nucleoid for Pom clusters at an off-center ... See full document
232
Two Lipid Signals Guide Fruiting Body Development of Myxococcus xanthus
... Real-time PCR. To quantify the mRNA levels of MXAN_2146, total RNA was extracted using RNAsnap (simple nucleic acid purification) from LS1191 mixed with different lipids at 24 h into development. Prim- ers for reverse ... See full document
8
Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy
... Previous studies that have used conventional optical microscopy methods to image gliding myxobacteria have also failed to observe changes in surface adhesion due to the elongation of the axial point spread function being ... See full document
13
Site specific receptor methylation of FrzCD in Myxococcus xanthus is controlled by a tetra trico peptide repeat (TPR) containing regulatory domain of the FrzF methyltransferase
... 1990) analysis of all sequenced bacterial genomes revealed that dozens of bacterial species possess putative methyltrans- ferases with one or more TPRs, including several within the a-proteobacteria, ... See full document
12
Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus
... In theory, any specific 20 nt sequence followed by a PAM can be used to target the DNA sequence for the Cas9-induced directed cleavage, but the cleavage efficien- cies of different spacers may be varied. There are three ... See full document
15
CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus
... M. xanthus strains containing mxdCas9-activator, plas- mids with sgRNA and the complete epothilone gene cluster were grown for 24 h in 50 mL CYE medium sup- plemented with Apra Tet and Km ...The ... See full document
12
Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in Myxococcus xanthus
... M. xanthus strains containing the complete epothilone gene cluster were grown overnight in 100 mL CYE medium supplemented with Apra and Cm ...The analysis was carried out on a Shim-pack MRC-ODS RP ... See full document
13
The High-Mobility Group A-Type Protein CarD of the Bacterium Myxococcus xanthus as a Transcription Factor for Several Distinct Vegetative Genes
... and Gene carR codes for a membrane-spanning ...molecular analysis of these two responses have re- CarR acts as an anti--factor, sequestering CarQ to the vealed two complex networks of regulatory actions ... See full document
12
Towards the mechanism of carotenogenesis in Myxococcus xanthus
... Further analysis of membrane proteins showed, despite the lethality of acridine orange, carotenoids were still protecting membrane proteins, and that lethality was due to nucleic acid ... See full document
319
Ras GTPase like protein MglA, a controller of bacterial social motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio
... BMS_0053 shares only limited sequence similarity with other MglB Roadblock domain proteins (BMS_0053, 168 residues, shares 22% identity and 43% similarity (NEEDLE global alignment) with M. xanthus MglB protein, ... See full document
13
Phylogenetic Characterization of Canine Distemper Viruses Detected in Naturally Infected Dogs in North America
... phylogenetic analysis of the complete H gene sequences provided the most robust, definitive results of the three genes examined due to both the observed sequence divergence and the large number of ... See full document
9
Strain-level diversity of secondary metabolism in Streptomyces albus
... ic gene clusters whose putative products comprise all of the major classes of secondary metabo- lites (Table ...strain-specific gene cluster, which is consistent with Salinispora arenicola, ... See full document
14
Morphological Analysis of Water Bodies A Case of Bangalore
... An analysis of the land use change over the past 4 decades shows a consistent decline in Water Bodies and an increase in the Built up area as shown in Fig.2. A city that constituted as much as 5% in Lake Area is ... See full document
5
Genome and EST analyses and expression of a gene family with putative functions in insect chemoreception
... Phylogenetic analysis shows this CSP to be specific to the Drosophila genus (Figure ...antennal-specific gene; however, we did not find it in the antennal EST libraries although this may be because of the ... See full document
14
Cloning, Sequencing and Phylogenetic Analysis of Stn Gene of Salmonella Typhimurium
... as putative virulence factor of Salmonella and causative agent of diarrhoea (Chopra et al ...stn gene did not resulted into reduction of virulence of Salmonella (Watson et ...stn gene is distributed ... See full document
7
The Role of Green Fluorescent Protein (GFP) in Transgenic Plants to Reduce Gene Silencing Phenomena
... There were three reasons Prasher thought that GFP could potentially become a significant tracer molecule. Firstly, if enough protein with attached GFP were made, it should be easy to detect and to trace it as it moved ... See full document
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