[PDF] Top 20 Analysis of recombinant adeno-associated virus packaging and requirements for rep and cap gene products.
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Analysis of recombinant adeno-associated virus packaging and requirements for rep and cap gene products.
... Hirt analysis was performed on samples from small-scale tran- sient transfections such as those described above to assay rep- lication of the vectors and to assess levels of wt AAV contam- ...full-length ... See full document
9
Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer
... Adeno-associated virus type 2 (AAV-2) is a nonpathogenic (10, 11), ...DNA virus that requires coin- fection with helper adenovirus or herpes simplex virus for efficient replication (5, ... See full document
11
Encapsidation of adeno-associated virus type 2 Rep proteins in wild-type and recombinant progeny virions: Rep-mediated growth inhibition of primary human cells.
... simian virus 40 (SV40) large T antigen were kindly provided by David ...for packaging into AAV ...of recombinant AAV by supplying rep and cap genes in ...trans. Recombinant AAV ... See full document
11
Characterization of Adenovirus-Induced Inverted Terminal Repeat-Independent Amplification of Integrated Adeno-Associated Virus rep-cap Sequences
... Stable packaging cell lines expressing the rep and cap genes for recombinant adeno-associated virus type 2 (rAAV-2) assembly constitute an attractive alternative to ... See full document
9
Adeno-Associated Virus (AAV) Rep Protein Enhances the Generation of a Recombinant Mini-Adenovirus (Ad) Utilizing an Ad/AAV Hybrid Virus
... AAV Rep protein, would allow for the excision of the AAV TR/insert from the recombinant Ad genome and packaging into recom- binant ...by products of first-generation Ad/AAV hybrid virus ... See full document
9
Control of adeno-associated virus type 2 cap gene expression: relative influence of helper virus, terminal repeats, and Rep proteins.
... the Rep proteins from the plasmid without the terminal repeats was about twofold lower than expression from the plasmid with terminal repeats and was completely dependent on adenovirus coinfection ...in ... See full document
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Novel Strategy for Generation and Titration of Recombinant Adeno-Associated Virus Vectors
... Adeno-associated virus (AAV) is a member of the depen- dent parvovirus family that requires coinfection with a helper virus, such as adenovirus or herpesvirus, to undergo a produc- tive ... See full document
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Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and Cap.
... helper virus and the virus generally used to produce ...cellular gene expression and regulation of rep expression ...AAV-2 Rep proteins inhibit Ad replication (44). Like Ad, herpes ... See full document
10
In Vitro Packaging of Adeno-Associated Virus DNA
... for packaging of recombinant adeno-associated virus ...marker gene to mammalian cells. The packaging procedure requires the presence of both the AAV Rep and capsid ... See full document
7
Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins.
... the adeno-associated virus type 2 (AAV-2) rep and cap genes obtained during a productive infection of HeLa cells with AAV-2 and adenovirus type 2 were fractionated according to ... See full document
9
Role for highly regulated rep gene expression in adeno-associated virus vector production.
... Recombinant adeno-associated virus (rAAV) has many fea- tures of interest in the field of gene therapy (6, 14, 34, ...long-term gene transfer (22, 23, 32, 37, ...replication, ... See full document
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Studies of the Mechanism of Transactivation of the Adeno-Associated Virus p19 Promoter by Rep Protein
... During adeno-associated virus (AAV) type 2 productive infections, the p19 promoter of AAV is activated by the AAV Rep78 and Rep68 ...proteins. Rep-induced activation of p19 depends on the ... See full document
11
Binding sites for adeno-associated virus Rep proteins within the human genome.
... a Rep nicking site nearby (Fig. 3) may make AAVS1 a preferred Rep-dependent integration site ...for Rep close to the RRS core sequence of any of the newly identified ...mal Rep-dependent ... See full document
7
Mutational analysis of adeno-associated virus Rep protein-mediated inhibition of heterologous and homologous promoters.
... point-mutated Rep proteins on heterologous and homol- ogous ...of recombinant Rep ...(B) Rep protein ...the Rep proteins were detected by immunoblotting with a monoclonal Rep ... See full document
12
Characterization of cell lines that inducibly express the adeno-associated virus Rep proteins.
... The fact that the Rep78-expressing neo6 and neo4O cells divided more slowly than the control neo5 cells may be due to the presence of the rep gene or, perhaps, a low, yet undetectable le[r] ... See full document
10
Transduction with recombinant adeno-associated virus for gene therapy is limited by leading-strand synthesis.
... Adeno-associated virus (AAV) is a human parvovirus being considered as a gene delivery vehicle for human gene ...nonenveloped virus contains a ...called rep and ... See full document
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Adeno-Associated Virus Small Rep Proteins Are Modified with at Least Two Types of Polyubiquitination
... of Rep could be di- rectly demonstrated by immunoblotting, using anti-Rep anti- body following immunoprecipitation of Rep, when highly sen- sitive detection methods were ...small Rep proteins ... See full document
6
Colocalization of adeno-associated virus Rep and capsid proteins in the nuclei of infected cells.
... By using a truncated AAV Rep protein expressed in Escherichia coli as an antigen, we obtained monoclonal antibodies that bind specifically to the two larger Rep proteins Rep78 and Rep68 [r] ... See full document
8
Characterization of Fabry mice treated with recombinant adeno-associated virus 2/8-mediated gene transfer
... A pair of Fabry mice, which were kindly provided by Dr. Roscoe O. Brady of the National Institutes of Health (Bethesda, MD, USA), were bred to acquire a sufficient number of mice for the study [16]. The mice were 18 ... See full document
10
Genetic Fate of Recombinant Adeno-Associated Virus Vector Genomes in Muscle
... long-lived gene expression in mouse muscle mediated by rAAV vectors, our original hypoth- esis was that rAAV vector genomes integrated in ...ciency virus DNA integration (3). In that human immunode- ... See full document
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