[PDF] Top 20 Rapid and Simple Approach for Identification of Mycobacterium tuberculosis Complex Isolates by PCR Based Genomic Deletion Analysis
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Rapid and Simple Approach for Identification of Mycobacterium tuberculosis Complex Isolates by PCR Based Genomic Deletion Analysis
... the Mycobacterium tuberculosis complex (TBC; M. tuberculosis, ...Comparative genomic analyses with the complete DNA sequence of M. tuberculosis H37Rv has provided information on ... See full document
7
Method for Rapid Identification and Differentiation of the Species of the Mycobacterium chelonae Complex Based on 16S 23S rRNA Gene Internal Transcribed Spacer PCR Restriction Analysis
... amplicon-restriction analysis assay, designated ITSPRA, which could rapidly and efficiently identify and differentiate the individual MCC ...confirmed based on the fact that similar results on ... See full document
7
Pyrosequencing assay for rapid identification of Mycobacterium tuberculosis complex species
... some genomic markers such as pncA, mpt40, hupB, gyrB and wbbl1genes in addi- tion to the regions of difference (RD) have been success- fully used for the differentiation of MTC members through direct ... See full document
6
Multiplex Real Time PCR Assay for Rapid Identification of Mycobacterium tuberculosis Complex Members to the Species Level
... the identification of ...the identification of ...the deletion of RD1, along with the expected peak at ...for analysis of RD9, RD1, and RD4 can differentiate M. tuberculosis, ... See full document
6
Rapid Detection of Isoniazid Resistance in Mycobacterium tuberculosis Isolates by Use of Real Time PCR Based Melting Curve Analysis
... INH-resistant isolates, whether it is necessary that the inhA S94A mutation be included in this assay is doubtful, in particular, when considering that this single mutation necessi- tates a probe and a ... See full document
9
PCR Based Method To Differentiate the Subspecies of the Mycobacterium tuberculosis Complex on the Basis of Genomic Deletions
... going tuberculosis-related projects revealed the potential of Rv0577 as an MtbC-restricted ...a PCR fragment (Table ...the PCR product from M. tuberculosis H37Ra was sequenced (data not ... See full document
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Direct Application of the INNO LiPA Rif TB Line Probe Assay for Rapid Identification of Mycobacterium tuberculosis Complex Strains and Detection of Rifampin Resistance in 360 Smear Positive Respiratory Specimens from an Area of High Incidence of Multidrug Resistant Tuberculosis
... M. tuberculosis complex isolates shown to be resistant to RIF by the standard suscep- tibility test were detected by the INNO-LiPA ...(29 isolates), H526Y, and D516V (1 isolate ... See full document
5
Novel DNA Chip Based on a Modified DigiTag2 Assay for High Throughput Species Identification and Genotyping of Mycobacterium tuberculosis Complex Isolates
... volume PCR was composed of 1⫻ KAPA2G Robust HotStart ready mix with dye (KAPA Biosystems, Woburn, MA) containing KAPA2G Robust HotStart DNA polymerase in an appropriate reaction buffer (including ...spp. ... See full document
7
Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis
... Our analysis of polymorphic nucleotide 285 in the oxyR genes of 360 ...M. tuberculosis complex organisms from wide- spread geographic sources and host species was 100% sensitive and specific for ... See full document
5
Differentiation of strains in Mycobacterium tuberculosis complex by DNA sequence polymorphisms, including rapid identification of M bovis BCG
... The Mycobacterium tuberculosis complex includes M. tuberculosis, ...M. tuberculosis complex were sequenced in a region of about 300 bp which contains multiple 15-bp tandem ... See full document
5
Role of CBNAAT in Detecting MDR Tuberculosis in Northern India
... of Tuberculosis is Mycobacterium tuberculosis, but other species, such ...the Mycobacterium tuberculosis complex (MTBC) ...[2]. Mycobacterium tuberculosis is a ... See full document
5
Use of a Mycobacteriophage Based Assay for Rapid Assessment of Susceptibilities of Mycobacterium tuberculosis Isolates to Isoniazid and Influence of Resistance Level on Assay Performance
... 20 isolates exhibiting high-level resistance (MIC ⱖ 2 g/ml) were scored as resistant by the MBA (100% concordance) (Table 2), while 6 of the 23 low-level-resistance strains (MICs, ...20 isolates (95%) ... See full document
5
Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR
... fg genomic DNA for M. tuberculosis (H37Rv) and 50 fg for ...the PCR products from the MTC strains gave expected banding patterns in correct sizes (Table ...M. tuberculosis in clinical ... See full document
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Stability of Variable Number Tandem Repeats of Mycobacterial Interspersed Repetitive Units from 12 Loci in Serial Isolates of Mycobacterium tuberculosis
... powerful approach to monitor- ing infectious ...is based on the assumption that patients infected with strains of identical types are epidemiologically linked, while those infected with strains of different ... See full document
6
Systems Biology Based Identification of Mycobacterium tuberculosis Persistence Genes in Mouse Lungs
... Identifying Mycobacterium tuberculosis persistence genes is important for developing novel drugs to shorten the du- ration of tuberculosis (TB) ...M. tuberculosis genes required for long-term ... See full document
10
Clinical utility of RD1, RD9 and hsp65 based PCR assay for the identification of BCG in vaccinated children
... assays based on the RD deletion profiles have been invaluable in differentiating members of the TBC ...16 isolates submitted for testing were positive for RD1 or RD9, indicating they were ...These ... See full document
6
The occurrence and frequency of genomic mutations that mediate Isoniazid and Rifampicin resistance in Mycobacterium tuberculosis isolates from untreated pulmonary Tuberculosis cases in urban Blantyre, Malawi
... low levels of drug-resistant MTB have been reported. A 2012 phenotypic culture-based large-scale survey in several districts of Malawi indicated MDR-TB levels of <0.4% among new TB cases and 4.8% among ... See full document
5
Pyrosequencing for Rapid Detection of Extensively Drug Resistant Mycobacterium tuberculosis in Clinical Isolates and Clinical Specimens
... a rapid effective method for identifying MTBC and detecting drug resistance mu- tations from clinical specimens and ...from PCR to PSQ, is ...resistance. Based on the outcome of this study, a larger ... See full document
8
Molecular Fingerprinting of Mycobacterium bovis subsp caprae Isolates from Central Europe
... 79 isolates revealed 11 different spoli- gotypes (designated C1 to C11), the profiles of which are schematically depicted in ...but isolates of C3, C6, C7, C10, and C11 are described here for the first ... See full document
5
Simple Multiplex PCR Assay for Identification of Beijing Family Mycobacterium tuberculosis Isolates with a Lineage Specific Mutation in Rv0679c
... lineage identification by multiplex PCR. Multiplex PCR was developed targeting the Beijing-specific SNP on Rv0679c, employing a primer with the mutated nucleic acid at the 3= end of the sequence ... See full document
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