[PDF] Top 20 Assembly of vesicular stomatitis virus: distribution of the glycoprotein on the surface of infected cells.

... PENHOET* Department of Biochemistry, University of California at Berkeley, Berkeley, California 94720 This study demonstrates that the glycoprotein of vesicular stomatitis virus clusters[r] ... See full document

... However, tunicamycintreated tsO110-infected cells incubated at 30°C do not release virus particles 5, the G protein is barely detectable at the cell surface 5, and there was little Gs sh[r] ... See full document

... the distribution of VSV G protein has any consequences on polarized virus re- lease, 9 h postinfection the apical and basolateral supernatants of filter-grown MDCK-I cells were plaque titrated (Table ... See full document

... were infected after polarization on collagen- coated Transwell supports, with polarity demonstrated by a trans- epithelial resistance (TER) of ⬎ 1,000 ⍀ and confirmed by proper localization of the apical ... See full document

... Apparently, glycosylation alone is not sufficient to provide the conformation required to expose the ASA sequence to anti-FMDV antibody. We had previously shown that chaper- ones or other posttranslational factors may be ... See full document

... Previous studies have ruled out degradation of virions or cell lysis as possible sources for extracellular soluble proteins 22; therefore, these proteins must be shed from infected cells[r] ... See full document

... In this assay, transient expression of wild-type VSV G protein from a cDNA clone resulted in the rescue of infectious virus particles from cells infected with a temperature-sensitive mut[r] ... See full document

... Perneable cells represent the only in vitro system described so far in which unequivocal genome replication and nucleocapsid synthesis have been shown to occur.. Previous cell-free RNA-s[r] ... See full document

... To determine when the nucleocapsids associated with the cytoskeletal framework during assembly, we harvested 32p_ labeled VSV-infected cells at 6.5 h postinfection and kept the Triton-so[r] ... See full document

... VSV-infected HeLa cells were, therefore, labeled with one of four different sugar precursors 3H-labeled glucosamine, mannose, galactose, or fucose for periods ranging from 5 to 60 min, a[r] ... See full document

... All three peptides recognized by H-2b mice and both epitopes recognized by H-2d mice which were characterized in primed T-cell populations were capable of activating specific Th cells in[r] ... See full document

... Characterization and Translation of Methylated and Unmethylated Vesicular Stomatitis Virus mRNA Synthesized In Vitro by Ribonucleoprotein Particles from Vesicular Stomatitis Virus-Infect[r] ... See full document

... To determine whether G protein trimers were in equilibrium with monomers in other cellular compartments, we studied heterotrimer formation between VSV G protein and a mutant G protein G,[r] ... See full document

... LODISH Department ofBiology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 Maturation of viral proteins in cells infected with mutants of vesicular stomatitis vir[r] ... See full document

... The isolation of a cytorelease of nucleoprotein into the cytoplasm plasmic membrane preparation from infected could not be observed by electron microscopy host cells which contained both[r] ... See full document

... Influence of multiplicity of infection on production of interferon in chick embryo cell cultures infected with S2 VS V or L1 VS V Multiplicity of infection 100 10 1 0.1 0.01 Interferon t[r] ... See full document

... All varieties of defective particle interfered with the multiplication of mutant and wild-type virus at the permissive temperature at early times of infection but failed to complement vi[r] ... See full document

... While the VSV-G protein clearly binds to PS, as demon- strated by others, this interaction does not appear to be rele- vant for VSV binding to cells, suggesting the presence of an undetermined cell surface ... See full document

... virions 500 Mg of viral proteins were in labeling of the NINS and L bands in addition labeled approximately with 1.5 mM TNBS in PBS at room temperato the remaining M protein, as shown in[r] ... See full document

... Results presented in this communication show that VSV proteins synthesized in vitro concurrently with genomic RNA assembled into structures that i banded in CsCl gradients or eluted from[r] ... See full document