[PDF] Top 20 Adeno-associated Virus (AAV) Assembly-Activating Protein Is Not an Essential Requirement for Capsid Assembly of AAV Serotypes 4, 5, and 11

... each AAV vector plasmid could provide a measure of AAV vector yield from each individual transfection by means of AAV ...separate AAV barcode-Seq studies as described ...the AAV vector ... See full document

... of AAV capsid ...of AAV capsids from the nucleoli (14, 20, ...the assembly process takes place ...for capsid formation. A role of the nucleolar capsid-interacting proteins in the ... See full document

... Adeno-associated virus (AAV) capsid assembly requires expression of the assembly-activating protein (AAP) together with cap- sid proteins VP1, VP2, and ... See full document

... most serotypes are compatible with VPs of heterologous serotypes to assemble capsids in the absence of their cognate AAP, albeit to various degrees (15, 19); this promiscuity of AAP-VP compatibility across ... See full document

... VP protein levels did not result in intact vector particles for any of the serotypes studied here, implying that VP proteins do not spontaneously self-assemble and form infectious particles in cells once a ... See full document

... universal AAV recep- tor, which was identified with high-affinity to multiple AAV serotypes including AAV2 48 ...VP1 capsid protein 49,50 ... See full document

... infectious AAV particles is reported to range from ...empty AAV particles have been shown to have a dens- ity corresponding to ...hand, AAV empty particles purified from insect cells have a density ... See full document

... Adeno-associated virus (AAV) is gaining momentum as a gene therapy vector for human ...this virus as a ...the virus, including nuclear targeting of the incoming virion during ... See full document

... for AAV capsid assembly and the determinants of serotype ...restore capsid assembly (to ...AAV1 capsid assembly compared to other N-terminal ...since capsid ... See full document

... AAV5 capsid VP proteins to the nucleolus, the organelle that is believed to be important for AAV capsid assembly, at least for AAV2 ...viral capsid proteins assem- ble if they are not ... See full document

... stimulates capsid assembly outside the nucleoli, possibly by recruitment of assembly fac- tors at these sites, cannot be ...an assembly pathway which obligatorily goes through the nucleolus in ... See full document

... In the case of the AAV4 and AAV5 HI loops, significant phenotypic variations were seen, possibly due to lower se- quence homology with AAV2. For instance, the AAV4 HI loop is composed of a higher number of hydrophobic ... See full document

... the AAV integration locus was cloned into pUC19, producing pMAT50 ...binding protein (MBP) expression plasmid pMAL-c2 by PCR amplification of AAV5 DNA (nucleotides [nt] 359 to 3600) with Pfu polymerase ... See full document

... decade, AAV vectors have emerged as leading gene delivery tools for therapeutic applications and biomedical ...the AAV life cycle, including how AAV interacts with host cellular factors to facilitate ... See full document

... Received 5 August 2002/Accepted 24 September 2002 Marker rescue, the restoration of gene function by replacement of a defective gene with a normal one by recombination, has been utilized to produce novel ... See full document

... The adeno-associated viruses (AAVs) being developed as vectors for corrective human gene delivery have shown promise in clinical trials, but preexisting antibodies are detrimental to successful ...on ... See full document

... Received 11 September 2002/Accepted 16 December 2002 The adenovirus L1 52/55-kDa protein is required for viral DNA packaging and interacts with the viral IVa2 protein, which binds to the viral ... See full document

... Sigma). All neutralization reactions were performed in triplicate. Samples of EMEM-0.1% BSA and mouse serum alone were included as controls. Ten microliters of diluted AAV lacZ vector was mixed with 10 ␮l of ... See full document

... cellular extracts for resolution, purified Rep68 protein was added to in vitro resolution reaction mixtures which con- tained extracts from uninfected or adenovirus-infected HeLa cells ([r] ... See full document

... The assembly of KSHV capsids is thought to occur in a manner similar to that determined for herpes simplex virus type 1 ...KSHV capsid open reading frames were cloned and the proteins expressed in ... See full document