[PDF] Top 20 Biogenesis of the Semliki Forest Virus RNA Replication Complex
Has 10000 "Biogenesis of the Semliki Forest Virus RNA Replication Complex" found on our website. Below are the top 20 most common "Biogenesis of the Semliki Forest Virus RNA Replication Complex".
Biogenesis of the Semliki Forest Virus RNA Replication Complex
... Antisera. A rat monoclonal antibody (MAb) (BU1/75 [ICR1]; Harlan Sera- Lab Ltd., Loughborough, United Kingdom) against bromodeoxyuridine (BrdU) was used to detect SFV RNA metabolically labeled with bromo-UTP ... See full document
12
Role of the Amphipathic Peptide of Semliki Forest Virus Replicase Protein nsP1 in Membrane Association and Virus Replication
... Mutants R253A and L255A ⫹ L256A behaved in an inter- esting intermediate fashion. They slowly gave rise to CPE, the initial signs of which were visible at 48 h. The titers of the viruses in the medium also rose slowly, ... See full document
12
Replicon Vectors Derived from Sindbis Virus and Semliki Forest Virus That Establish Persistent Replication in Host Cells
... Sindbis virus (SIN), Semliki Forest virus (SFV), and Venezuelan equine encephalitis virus, are widely used for gene expression studies in vitro and are being developed for both vaccine ... See full document
6
bcl-2 acts early to restrict Semliki Forest virus replication and delays virus-induced programmed cell death.
... viral RNA were double labeled for viral protein and TUNEL, and the number of blebbing cells was recorded (Table ...viral RNA-positive and viral glycoprotein-positive cells in SFV A7-infected AT3Neo cultures ... See full document
8
Studies on defective interfering Semliki Forest virus
... of virus, in mice infected by different virus variants, showed that virus titres were similar for the first hours after infection and it was only after approximately 48 h that differences became ... See full document
295
Magnetic Fractionation and Proteomic Dissection of Cellular Organelles Occupied by the Late Replication Complexes of Semliki Forest Virus
... several RNA-binding pro- teins, including many hnRNPs, are reproducibly identified as in- ...the replication not only of alphaviruses but also of RNA viruses in ...one virus are not always ... See full document
18
Nairovirus RNA Sequences Expressed by a Semliki Forest Virus Replicon Induce RNA Interference in Tick Cells
... cephalitis virus, Crimean-Congo hemorrhagic fever virus, Lyme borreliae, and ...the virus in its vector. We report here the use of recombinant Semliki Forest virus to induce ... See full document
6
Critical residues of Semliki Forest virus RNA capping enzyme involved in methyltransferase and guanylyltransferase-like activities.
... Sindbis virus nsP1 and their effect on MT activity in ...the virus genome, implying that capping of viral mRNAs is necessary for successful virus ... See full document
6
Human MxA Protein Confers Resistance to Semliki Forest Virus and Inhibits the Amplification of a Semliki Forest Virus-Based Replicon in the Absence of Viral Structural Proteins
... various RNA and DNA viruses revealed that MxA-expressing HEp-2 cells were protected not only against influenza virus and vesicular stomatitis virus (VSV) but also against Semliki Forest ... See full document
7
Attenuation of Semliki Forest Virus Neurovirulence by MicroRNA-Mediated Detargeting
... their replication in unwanted tissues, such as the liver. Semliki Forest virus (SFV) is a positive-strand RNA virus and, similar to the related alphaviruses, like Sindbis ... See full document
10
The nucleocapsid-binding spike subunit E2 of Semliki Forest virus requires complex formation with the E1 subunit for activity.
... samples suggested that it was the unprocessed p62, and not E2*, which aggregated. The pulse-chase analyses showed that the level of aggregation increased with time. After a 5-min chase, very few aggregates were found ... See full document
9
Efficient multiplication of a Semliki Forest virus chimera containing Sindbis virus spikes.
... single-stranded RNA genome, which is 11 to 12 kb in length, has been at least partially sequenced for ten mem- ...viral RNA genome and not to ...encephalitis virus (WEE), which has been shown to have ... See full document
6
Following acute encephalitis, Semliki Forest virus is undetectable in the brain by infectivity assays but functional virus RNA capable of generating infectious virus persists for life
... Semliki Forest virus (SFV) provides a well-characterised and tractable model of virus encephalitis ...which virus crosses the BBB to establish small perivascular foci of CNS infection ... See full document
13
Properties and use of novel replication competent vectors based on Semliki Forest virus
... groups: virus-like particles (VLPs), layered DNA-RNA vectors, and replication-competent vectors ...replicon RNA, which represents the alphavirus genome in which the region coding for struc- ... See full document
16
Properly Folded Nonstructural Polyprotein Directs the Semliki Forest Virus Replication Complex to the Endosomal Compartment
... P1234, which consists of 2,432 aa. Recombinant baculoviruses expressing P1234, P123, P12, P23, and P34 (Fig. 1) were used to infect Trichoplusia ni (Tn5) cells. The expression of SFV- specific nsPs was detected in cells ... See full document
12
Replication of semliki forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA.
... A 1.2 minus strand of the total population of RFs, we wanted to determine whether the size of the polyU in SFV RFs was variable and in particular whether the polyU in the minus-strand RN[r] ... See full document
19
Replication of Semliki Forest virus RNA in infected chick cells
... Estimation of the time required to synthesize a molecule of viral RNA As explained earlier Discussion, Section III 1, if RNA is being synthesized and labelled at a constant rate, the lab[r] ... See full document
177
Template RNA Length Determines the Size of Replication Complex Spherules for Semliki Forest Virus
... of RNA replication levels or spherule ...the virus-specific se- quences, as described in Materials and ...well. Replication initiated by linearized template to- gether with circular replicase ... See full document
10
Replicase Complex Genes of Semliki Forest Virus Confer Lethal Neurovirulence
... viral RNA species with the deletion was the only RNA present in our original A7(74) virus ...Middelburg virus (43), Ross River virus (42), and Venezuelan (16) and western and eastern ... See full document
11
Semliki Forest Virus Budding: Assay, Mechanisms, and Cholesterol Requirement
... progeny virus production (data not shown; see also reference ...in virus particles was measured by mag-SA retrieval ...into virus particles was observed at infection times when the degradation of ... See full document
12
Related subjects