[PDF] Top 20 The Buoyancy of Cryptococcus neoformans Is Affected by Capsule Size

... the size (diameter) for a spherical object such as a yeast cell (43, ...variable size (3 to 16 ␮ m in diameter) of C. neoformans grown in MM and the low density ( ⬃ ...C. neoformans would be ... See full document

... C. neoformans strains may be related to the ability of tannins to inhibit extracellular microbial enzymes, dep- rivation of substrates and metal-ion cell-envelope trans- port proteins and the direct action on ... See full document

... High content imaging method. Infected zebrafish were anesthetized by immersion in 0.168 mg/mL tric- aine E3 and mounted in agar channels for imaging. Channels were made by adding 200 μ l of 1% agar (Cat. No. 05039) in E3 ... See full document

... of capsule decrease after gamma radiation treat- ...C. neoformans cells results in capsular polysaccharide release ...the capsule in a graded fashion. The capsule of C. neoformans ... See full document

... Capsule protected cells against ionizing radiation. The cap59 strain set was irradiated to determine whether the cap- sule protected the fungus against ionizing radiation. The sur- vival of acapsular cap59 mutant ... See full document

... in capsule-inducing medium (see Materials and Methods) showed slightly more GXM in the mutant CM, although this amount was still significantly less than that seen in wild-type CM ...a capsule donor as ... See full document

... a size suitable for ...the capsule provided evidence that the negative charge of the capsule was not evenly distributed ...the capsule could be the result of homopolymeric tracts of M6, which ... See full document

... of Cryptococcus has been documented in several cases of recurrent infection as well as by in vitro and in vivo experiments ...reduced capsule size and altered carbon source ...of Cryptococcus ... See full document

... C. neoformans remain to be char- acterized in ...for capsule assembly and the genes encoding laccases for melanin ...influence capsule attachment to the cell wall via regula- tion of the ... See full document

... Cryptococcus neoformans is variably encapsulated in vitro, whereas in tissues it develops a large ...[DME]). Capsule size was quantitated physically by measuring cell volume, and chemically by ... See full document

... cell size can have an impact on phagocytosis (described for titan cells ...on capsule structure, as shown here by the differ- ence in serotype assignment achieved with a monoclonal antibody ... See full document

... induce capsule growth. The mechanism by which iron limitation induces capsule growth remains ...the capsule, an increase in capsule size would be a response to transport iron more ... See full document

... in capsule radius per unit of time was directly proportional and/or controlled by a single parameter (alpha), rep- resenting the relative growth rate of capsule to cell ...the capsule radius ... See full document

... hypothesis, capsule growth should stop or dimin- ish during budding, so we used time-lapse microscopy to visualize capsule ...The capsule, however, is not visible under regular conditions given its ... See full document

... a capsule around acapsular mutants (19). These vesicles vary in size, some being up to 200 nm in diameter, and are heterogeneous in ultrastructural morphol- ogy, a hint that there might be different types ... See full document

... C. neoformans PKA1 gene product has a clear role in capsule production (33), sinces a pka1 strain is acapsular and ...case, capsule production is not restored by exogenous cAMP, sug- gesting that PKA ... See full document

... exact test with data fit to a negative binomial distribution of the DESeq package (26) was used to identify the differentially ex- pressed (DE) genes. We made pairwise comparisons between dif- ferent conditions, and the ... See full document

... C. neoformans inoculated via the intranasal or intravenous route can result in mixed infec- tions in the lungs and/or brain (15, 38, ...C. neoformans (both serotypes A and D), genetically unrelated isolates ... See full document

... RNA-seq and data analysis. Total RNAs prepared as described above were purified with a com- mercial kit (RNeasy minikit; Qiagen). The concentration was measured with RNA detection dye (Quant-IT RiboGreen; Invitrogen). The ... See full document

... to the cytosol, which is used by the CIA machinery for the synthesis and maturation of cytosolic and nuclear Fe-S proteins (29, 30, 32). S. cerevisiae cells depleted of Atm1 have reduced growth rates and reduced ... See full document