[PDF] Top 20 The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.
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The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.
... the PB1 and PA subunits of RNA poly- merase and NP can support the replication of the influenza virus genome ...of PB1 and PA in replication, ... See full document
5
A Single Amino Acid Mutation in the PA Subunit of the Influenza Virus RNA Polymerase Inhibits Endonucleolytic Cleavage of Capped RNAs
... mRNA, cRNA, and vRNA by polymerases with mutant PA ...their PA genes has indicated that PA is involved in replication, but not necessarily in the transcriptional activity of the ... See full document
13
Functional Analysis of PA Binding by Influenza A Virus PB1: Effects on Polymerase Activity and Viral Infectivity
... A virus expresses three viral polymerase (P) subunits—PB1, PB2, and PA—all of which are essential for RNA and viral ...and replication have been partially elucidated, yet ... See full document
10
Correlated mutations in the four influenza proteins essential for viral RNA synthesis, host adaptation, and virulence: NP, PA, PB1, and PB2
... single-stranded RNA gene segments in the influenza A virus, which are present as ribonucleo- protein complexes (vRNPs) with nucleoprotein (NP) and polymerase within the virus ...The ... See full document
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The Replication Activity of Influenza Virus Polymerase Is Linked to the Capacity of the PA Subunit To Induce Proteolysis
... nonproteolytic PA proteins are able to transcribe but unable to ...of viral polymerase with nonproteolytic PA mutants could be the con- sequence of either reduced replication, reduced ... See full document
6
Involvement of Influenza Virus PA Subunit in Assembly of Functional RNA Polymerase Complexes
... of PA in ts53 virus- infected cells at ...ts53 virus-infected cells at 34°C ...the viral protein synthesis decreased slightly in ts53 virus-infected cells at ...that PA is ... See full document
Threonine 157 of Influenza Virus PA Polymerase Subunit Modulates RNA Replication in Infectious Viruses
... of PB1 that is involved in PA interaction overlaps the PB1 region that is responsible for the binding of cRNA and vRNA molecules (12, 33, ...Although PB1 alone can bind ... See full document
7
cis-Acting Packaging Signals in the Influenza Virus PB1, PB2, and PA Genomic RNA Segments
... the influenza virus PA, PB1, and PB2 genome segments, we have mapped cis-acting sequences that are necessary and suf- ficient to enable each of these segments to be packaged effi- ... See full document
8
The influenza A virus PB2 polymerase subunit is required for the replication of viral RNA.
... a cRNA template ...this subunit can be cross-linked to cap analogs (5, 47, 51), antibodies against PB2 protein affect transcription initiation (2) or endonuclease activity (29) in vitro, and ... See full document
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The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis.
... the PB1 and PA subunit genes and the NP gene can be expressed in response to dexamethasone ...the PB1, PA, and NP genes in clone 64 cells are almost the same as those in clone 76 ... See full document
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Synthesis of the small subunit of ribulose 1,5 bisphosphate carboxylase
... envelope can be isolated from leaf tissue if prepared rapidly and with an isolation medium which contains a suitably high osmotic strength (Ramirez e£ ...chloroplasts can then be employed to study either ... See full document
376
Influenza Virus PB1 and Neuraminidase Gene Segments Can Cosegregate during Vaccine Reassortment Driven by Interactions in the PB1 Coding Region
... Egg-grown influenza vaccine yields are maximized by infection with a seed virus produced by “classical reassortment” of a sea- sonal isolate with a highly egg-adapted ...polymerase subunit PB1 ... See full document
10
Sequence in the Influenza A Virus Nucleoprotein Required for Viral Polymerase Binding and RNA Synthesis
... the viral polymerase has the inherent capacity to initiate unprimed vRNA synthesis, but it synthesizes only short transcripts (approximately 20 bases long) that do not extend past the surface of the ... See full document
6
Cellular Protein HAX1 Interacts with the Influenza A Virus PA Polymerase Subunit and Impedes Its Nuclear Translocation
... A virus is an orthomyxovirus and an important patho- gen of humans and ...The viral genome consists of eight segments of negative-sense single-stranded RNA which are encap- sidated as ... See full document
14
Studies on influenza A virus PB1 F2 protein
... reduced viral growth (Figure ...in viral growth when cells were infected with a low MOI (Figure ...measure viral growth in immune incompetent ...MOI can support production of defective ... See full document
241
The RNA Subunit of Telomerase Is Encoded by Marek's Disease Virus
... MDV genome, we defined its promoter region between the MDV telomeric sequences and the cTR transcription start ...the viral sequences (positions 388 to ...the viral telomerase RNA gene in very ... See full document
12
RNA subunit structure of Mason-Pfizer monkey virus.
... Dissociation of 60-70S RNA of MasonPfizer monkey virus and murine leukemia virus by heat or formamide 40% resulted in conversion to identical subunit structures of 2.8 x 106 daltons; tre[r] ... See full document
5
Inhibition of Human Cytomegalovirus pUL89 Terminase Subunit Blocks Virus Replication and Genome Cleavage
... We can more quantitatively measure one or a limited number of cleavage events using a 60-bp dsDNA substrate with the ELISA described here than is possible with larger DNA ...other viral proteins remains ... See full document
14
The Pre-NH2-Terminal Domain of the Herpes Simplex Virus 1 DNA Polymerase Catalytic Subunit Is Required for Efficient Viral Replication
... recombinant baculovirus that was engineered in a similar fashion (kindly provided by Gloria Komazin-Meredith). Viral titers were determined us- ing a BacPAK baculovirus rapid titer kit (Clontech). Mid-log-phase ... See full document
9
KILLED SUBUNIT INFLUENZA VACCINE IN CHILDREN
... The two chil- dren who failed to develop significant anti- body rises had no preexisting antibody and developed detectible antibody ( 1 : 10) after a single vaccine dose.. This did not r[r] ... See full document
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