[PDF] Top 20 Characterization of Dengue Virus Complex-Specific Neutralizing Epitopes on Envelope Protein Domain III of Dengue 2 Virus

... of epitopes mapped for other DENV ED3 complex- and subcomplex-specific neutralizing ...DENV complex- specific MAb 4E11 has an epitope on the DENV-1 ED3 pro- tein that includes ... See full document

... group-specific epitopes poses the follow- ing ...recognize epitopes con- served among serotypes (e.g., those in domain II) also cross- neutralizing at a reasonable titer, or are ... See full document

... Domain III of dengue virus E protein (DIII) participates in the recognition of cell receptors and in structural rearrangements required for membrane fusion and ultimately viral ... See full document

... recombinant protein, since the determination was not performed at day 0 of the booster ...chimeric protein, demonstrat- ing that epitopes of the viral regions present in the protein are able ... See full document

... prM protein and is comprised largely of serotype cross-reactive and weakly neutral- izing antibodies ...surface-exposed epitopes, are se- rotype specific or potently inhibitory (neutralizing ... See full document

... Zika virus (ZIKV) is an emerging flavivirus that can cause birth defects and neurologic ...and dengue virus (DENV) widely cocirculate, distin- guishing ZIKV infection from DENV infection is ... See full document

... nonreplicating dengue virus type 2 (DENV-2) vaccines: (i) a DNA vaccine containing the prM-E gene region (D), (ii) a recombinant subunit protein vaccine containing the B domain ... See full document

... 18–20). Protein binding-based assays performed to distinguish DENV and ZIKV infections, while critical for accurately diagnosing infection history (12, 20), may oversimplify the com- plex neutralizing ... See full document

... E protein is a determinant of tropism and virulence, as well as the major target of neutralizing and enhancing anti- bodies to DENV (2, 9, 13, 26, ...E protein revealed three domains (domains ... See full document

... with dengue and West Nile virus are not mainly directed to well-studied neutralizing anti- body epitopes on EDIII (20, 27, ...recombinant protein antigens and the native protein ... See full document

... ABSTRACT Dengue virus serotype 2 (DENV2) is widespread and responsible for severe ...from dengue cases and structure-guided design of a chimeric DENV, here we de- scribe the major site on the ... See full document

... between neutralizing and en- hancing activities in serum is based on serum antibody species composition, the total antibody response induced by the E antigen molecule is affected by the ability of each epitope to ... See full document

... EDIII protein of DENV-1, DENV-2, DENV-3, and DENV-4 (1), separately or mixed together, were used to immunize BALB/c mice as described previously ...EDIII protein from each dengue serotype as ... See full document

... For each individual MAb, the DENV-2 DIII mutant library was screened according to a previously described protocol (53). To identify yeasts that had selectively lost binding to a given MAb, the library was ... See full document

... reacting protein was detected as a major band of 37 kDa in apparent molecular weight (Figure 2A, left panel), whereas it was visible as a blurred band at 72-75 kDa in VLPs (Figure 2A, right ...RIgE protein ... See full document

... induced protein band was confirmed using antibodies specific to each of the two fusion partners as well as to the affinity tag by immunoblotting analyses (Figure ... See full document

... encephalitis virus (JEV) in different cross-reactivity ...and neutralizing activity against DV1 and showed no cross-reactivity to DV2, DV3, DV4 or JEV-infected cells as characterized by ELISA, dot blot, ... See full document

... ABSTRACT Dengue virus is the most globally prevalent mosquito-transmitted vi- ...of neutralizing antibodies, promote uptake of antibody-opsonized virus in Fc- ␥ receptor-positive ...broadly ... See full document

... The initial description of EDE antibodies reported that they were immunodominant in nearly half of the study’s subjects (in- cluding one individual with a primary DENV infection), but there are several important caveats: ... See full document

... E protein, in which alanines inserted into the C terminus of T1 (corresponding to DENV E positions 457, 461, and 465) or T2 (DENV E positions 479, 483, and 487) helix did not affect the PrM-E interaction (53) and ... See full document