[PDF] Top 20 Characterization and mapping of a nonessential pseudorabies virus glycoprotein.

... 173 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Antigenic variants of pseudorabies virus PRV containing mutations in a viral glycoprotein with a molecular weight of[r] ... See full document

... 57 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A pseudorabies virus variant marl97-1 containing a mutation in a viral glycoprotein with a molecular weight of 50,000[r] ... See full document

... few nonessential glycopro- teins which is conserved in the ...gE/gI-negative virus mutant caused accumulations of nucleocapsids in association with tegument protein, and only very few virus particles ... See full document

... IMPORTANCE Herpesvirus infection requires fusion of the viral envelope with cellu- lar membranes, which involves the conserved fusion machinery consisting of gB and the heterodimeric gH/gL complex. The bona fide fusion ... See full document

... Given the general collinearity of alphaherpesvirus genomes, we determined the nucleotide sequence (36) of a portion of BamHI fragment 3 of PrV strain Ka (18) which was suspected to contain the PrV homolog of the HSV-1 ... See full document

... the nonessential gG gene, exhibits growth properties similar to those of the parental strain PrV-Ka ...this virus expresses a membrane-anchored gE which lacks the cytoplasmic domain ... See full document

... The crystal structures of the gH homologs of HSV-2, EBV, and PrV revealed a conserved subdomain organization of the gH ectodomain (29, 35, 36). However, despite these structural simi- larities and an essential function ... See full document

... Because the monoclonal antibodies against the major 98K glycoprotein do not coprecipitate the 130K, 115K, and 62K proteins which are complexed with the 98K glycoprotein, it appears that [r] ... See full document

... other nonessential glycoproteins, gC, gG, gM, and gN, delayed the appearance of the symptoms to a variable extent but did not abolish the neuroinvasiveness of PrV in adult ... See full document

... To localize the structural gene for gA, we used cloned BamHI fragments of the viral DNA to select specific mRNA species and immunoprecipitated their in vitro translation products with th[r] ... See full document

... Anatomy of the herpes simplex virus type 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants.. [r] ... See full document

... The gene coding for this processed protein and its lower-molecular-weight precursor was mapped within the small unique region of the genome by hybridization of mRNA to cloned fragments o[r] ... See full document

... Identification and expression of human cytomegalovirus glycoprotein with homology to the Epstein-Barr virus BXLF2 product, varicella-zoster virus gpIII, and herpes simplex virus type 1 g[r] ... See full document

... These results show that i gHPrV, as detected by our antisera in purified virion preparations, does indeed constitute a glycosylated protein; ii approximately S kDa of endo H-sensitive ca[r] ... See full document

... PrV glycoprotein mutants available in our laboratory (mutants lacking gB, gC, gD, gE, gG, gH, gI, gL, gM, or gN, as well as several double and triple mutants) were tested for expression of gK by West- ern blotting ... See full document

... in virus in- ...virions, virus maturation and egress, and direct viral cell-to-cell spread from infected to adjacent noninfected cells ...in pseudorabies virus (PrV), the essential gD is ... See full document

... Transneuronal spread of alphaherpesviruses is an incom- pletely understood multistep process that requires the con- certed action of viral and cellular proteins. Following replica- tion in the soma of an infected neuron, ... See full document

... To analyze whether PrV gB was able to complement the defect in cell-to-cell spread associated with lack of gB in HSV-1, gB- HSV-1 mutants that had been produced on cells expressing HSV-1[r] ... See full document

... varicella-zoster virus, egress of virions from infected cells has been postulated to involve primary envelopment at the inner nuclear mem- brane, loss of primary envelope by fusion at the outer lamella of the ... See full document

... While the majority of wild-type virions adsorb to the cells by the mode of adsorption that involves the interaction of viral gIll with the cellular heparinlike moiety, a small proportion[r] ... See full document