[PDF] Top 20 Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli
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Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli
... and C- terminally truncated NS5B were expressed as polyhistidine (His2)-tagged fusion ...the C-terminally tagged proteins are designated NS5B-His or ...the C- terminally truncated NS5B, ... See full document
6
Identification and Characterization of the Escherichia coli-Expressed RNA-Dependent RNA Polymerase of Bamboo Mosaic Virus
... concentration, 0.8 mM) was added to the culture when the cell density reached an optical density at 600 nm of approximately 0.8 to 1 to induce the expression of the lysogenic T7 RNA polymerase that, in ... See full document
7
Comparison of Turnip Crinkle Virus RNA-Dependent RNA Polymerase Preparations Expressed in Escherichia coli or Derived from Infected Plants
... Characterization of RdRp activities of TCV p88 and p88C. To test the stability and activity of p88 and p88C, we per- formed the RdRp assays at various temperatures, as shown in Fig. 4 using minus-strand satC ... See full document
11
Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase
... clone pJG-Sap01 (GenBank accession number AY694184) as described above. The PCR product was molecularly cloned into the pDEST14 vector (Gateway) according to the manufacturer’s instructions and using primers VZ5-Sap-3D- ... See full document
14
A Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase Capable of Copying the Full-Length Viral RNA
... NS5B expressed in insect cells (4, 23) and the C-terminus- truncated NS5B expressed in Escherichia coli (10, ...separate RNA or a folded-back 3⬘ end of the template as a ...HCV ... See full document
9
De Novo Initiation Pocket Mutations Have Multiple Effects on Hepatitis C Virus RNA-Dependent RNA Polymerase Activities
... from Escherichia coli. NS5B proteins were expressed from pET plasmid derivatives in ...E. coli BL21(DE3). The prototype HCV NS5B protein lacked the hydrophobic C-terminal 21 amino ...of ... See full document
11
Structure of Hepatitis C Virus Polymerase in Complex with Primer-Template RNA
... HCV polymerase genotype 2a JFH-1 isolate was designed with the C-terminal 21 amino acids removed and replaced with a noncleavable hexahistidine tag, and it was cloned into a pET-28a-derived ...(DE3) ... See full document
9
De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... the C-terminal 18 amino acids of NS5B have any effects on in vitro RdRp activity under the conditions used in this ...restored, expressed, and purified to ⬎80% homo- geneity (data not ...D(⫹) RNA as ... See full document
13
Mechanism of De Novo Initiation by the Hepatitis C Virus RNA-Dependent RNA Polymerase: Role of Divalent Metals
... from Escherichia coli. NS5B proteins were expressed in fermentors from pET derivatives of ...E. coli BL21(DE3)LysS. NS5B proteins from HCV, BVDV, and GB virus-B (GBV) were truncated at ... See full document
13
Interaction with a Ubiquitin-Like Protein Enhances the Ubiquitination and Degradation of Hepatitis C Virus RNA-Dependent RNA Polymerase
... Hepatitis C virus (HCV), a member of the Flaviviridae fam- ily, is the major causative agent of non-A, non-B hepatitis ...single-stranded RNA genome en- codes a single polyprotein of ... See full document
11
Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.
... To determine whether both p63 and NCVP2 are required for polymerase activity, we further purified the soluble polymerase and analyzed the viral proteins associated with the purified enzy[r] ... See full document
9
Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase
... the C- terminal membrane segment of human cytochrome b5 (Cb5), a prototype tail-anchored protein (8), did not yield any viable clones ...for RNA replication is absent in Cb5 or impeded by its rigid ... See full document
5
Biochemical characterization of the fidelity of poliovirus RNA dependent RNA polymerase
... Incorrect incorporation into Sym/Sub U. (A) Apparent inhibition by high NTP concentrations. Products formed in the presence of 3000 (circles), 5000 (squares) and 6000 (tri- angles) µM GTP were quantified and plotted as a ... See full document
11
Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... influenza RNA polymerase were performed as described previously ...influenza virus polymerase copurified with TAP-CTD was used to transcribe a short (14-nucleotide) viral RNA template ... See full document
7
Characterization of an RNA-dependent RNA polymerase activity associated with La France isometric virus.
... stranded RNA (ssRNA) as determined by cellulose column chromatography and ionic-strength-dependent sensitivity to hydrolysis by RNase ...The RNA products synthesized in vitro appeared to be of a ... See full document
6
Binding and transcription of simian virus 40 DNA by DNA-dependent RNA polymerase from Escherichia coli.
... These results suggest that all of the polymerase binding sites have similar affinities for polymerase under these conditions, also supporting the proposal that, under standard conditions[r] ... See full document
7
Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
... genomic RNA is sensitive to ...antigenomic RNA. In contrast, Macnaughton et al. obtained data that antigenomic RNA syn- thesis seemed resistant to amanitin, but we consider that the signals they ... See full document
10
Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro.
... the polymerase undergoes a conformational change(s) during transcription ini- ...the polymerase by binding to the PB1 subunit, which then is able to initiate transcription at the 3 9 terminus of the vRNA ... See full document
9
Zinc Salts Block Hepatitis E Virus Replication by Inhibiting the Activity of Viral RNA-Dependent RNA Polymerase
... be dependent on the level of zinc available before the onset of zinc ...capped RNA of the P6 HEV-Luc replicon, treated every day for 3 days with 100 M Zn sulfate and/or 10, 50 M ribavirin, as ... See full document
13
Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA
... viral RNA on the NS5B RdRp activity was not ...minus-strand RNA synthesis depends on an initial recognition and specific binding of the NS5B RNA polymerase or replicative complex to the 3⬘ ... See full document
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