[PDF] Top 20 Whole chromosomal DNA probes for rapid identification of Mycobacterium tuberculosis and Mycobacterium avium complex
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Whole chromosomal DNA probes for rapid identification of Mycobacterium tuberculosis and Mycobacterium avium complex
... Whole chromosomal DNA probes were used to identify clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium complex, and Mycobacterium gordonae.. tuberculosis was prepared fr[r] ... See full document
5
Novel DNA Chip Based on a Modified DigiTag2 Assay for High Throughput Species Identification and Genotyping of Mycobacterium tuberculosis Complex Isolates
... universal DNA chip for species identifica- tion of the MTBC and typing ...M. tuberculosis, the multiplex geno- typing conditions for 51 target SNPs were optimized using well- characterized ... See full document
7
Absence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Disease
... of Mycobacterium avium could be matched by DNA ...for Mycobacterium intracellulare, household water sources for 36 patients with Mycobacterium avium complex (MAC) lung ... See full document
6
Pyrosequencing assay for rapid identification of Mycobacterium tuberculosis complex species
... molecular identification as previously described [21] was used in this study ...provided DNA extracts of MTC organisms isolated from different animal species (cattle, badger, cat, dog, sea lion, wild boar, ... See full document
6
Pyrosequencing for Rapid Detection of Extensively Drug Resistant Mycobacterium tuberculosis in Clinical Isolates and Clinical Specimens
... (XDR) tuberculosis (TB) is a serious ...the rapid identification of Mycobacterium tuberculosis complex (MTBC) and concurrent de- tection of mutations associated with resistance ... See full document
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Direct identification of Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare from amplified primary cultures in BACTEC media using DNA probes
... intracellulare, hybridization results from the BACTEC material for the 190 cultures were derived by dividing the hybridization value obtained with the most reactive probe by the average [r] ... See full document
5
Differentiation of strains in Mycobacterium tuberculosis complex by DNA sequence polymorphisms, including rapid identification of M bovis BCG
... M. bovis BCG. PCR/REA provides a rapid, sensitive, and specific test for the identification of BCG. All 23 M. bovis BCG strains were sequevar MED-C (Table 4). These strains in- cluded 20 reference BCG ... See full document
5
Use of restriction fragment length polymorphisms resolved by pulsed field gel electrophoresis for subspecies identification of mycobacteria in the Mycobacterium avium complex and for isolation of DNA probes
... Mycobacterial strains from the Mycobacterium avium complex were compared with each other and with Mycobacterium phlei isolates by restriction endonuclease digestion of chromosomal DNA wi[r] ... See full document
8
Direct Detection of Mycobacterial Species in Pulmonary Specimens by Two Rapid Amplification Tests, the Gen Probe Amplified Mycobacterium tuberculosis Direct Test and the GenoType Mycobacteria Direct Test
... amplified DNA product is hybridized to specific oligonucleotide probes immobilized on the ...M. tuberculosis complex (MTC), Mycobacterium avium, My- cobacterium intracellulare, ... See full document
5
Loop Mediated Isothermal Amplification for Direct Detection of Mycobacterium tuberculosis Complex, M avium, and M intracellulare in Sputum Samples
... of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium intracellulare directly from sputum specimens as well as for detection of culture isolates grown in ... See full document
7
Direct Application of the INNO LiPA Rif TB Line Probe Assay for Rapid Identification of Mycobacterium tuberculosis Complex Strains and Detection of Rifampin Resistance in 360 Smear Positive Respiratory Specimens from an Area of High Incidence of Multidrug Resistant Tuberculosis
... M. tuberculosis and detection of rpoB gene muta- tions were made directly from acid-fast-bacillus (AFB)-positive respiratory specimens with the aid of the INNO-LiPA ...total DNA extract was used in a 50- ... See full document
5
Clinical utility of RD1, RD9 and hsp65 based PCR assay for the identification of BCG in vaccinated children
... the identification of members of TBC include (i) AccuProbe Mycobacterium Tubercu- losis Complex Culture Identification Test (Gen-Probe Incorporated, San Diego, CA) is a rapid DNA ... See full document
6
Identification of Mycobacterium avium complex in sarcoidosis
... M. tuberculosis or M. avium woodpigeon strains, ...mycobacterial DNA corresponding to the specificity of each assay (data not ...human chromosomal DNA by any of the assays when ... See full document
6
Identification of Mycobacterium tuberculosis and Mycobacterium avium M intracellulare directly from primary BACTEC cultures by using acridinium ester labeled DNA probes
... The BACTEC broth from specimens containing blood that were centrifuged without SDS-EDTA treatment and tested by using the AccuProbe system gave high hybridization signals in the absence [r] ... See full document
5
Novel Multiplex Real Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
... study, DNA was supplied by Mario Vaneechoutte and had been characterized using techniques previously described (10, 36, ...41). DNA isolation and quantification. Genomic DNA from NTM and 2 ...total ... See full document
7
Rapid method for testing susceptibility of Mycobacterium tuberculosis by using DNA probes
... tion method by the commercially available AccuProbe M. tuberculosis complex test (Gen Probe, Inc., San Diego, Calif.) (6, 8, 13) according to the instructions of the manufacturer. The test consists of the ... See full document
5
Deciphering drug resistance in Mycobacterium tuberculosis using whole-genome sequencing: progress, promise, and challenges
... M. tuberculosis is the result of chromo- somal mutations in existing genes that are passed along through vertical descent, that is, passed from mother to daughter ...M. tuberculosis rarely recombines via ... See full document
18
Comparative Evaluation of the New Version of the INNO LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains
... All 14 M. kansasii strains tested were correctly identified by both tests. Moreover, the LiPA Mycobacteria assay provided further differentiation of M. kansasii to different genotypes (2). One M. thermoresistibile strain ... See full document
6
Nontuberculous mycobacterial infection after lung transplantation: a report of four cases
... not detected in any prior to transplantation. Surveil- lance bronchoscopy examinations were performed four times the first year and then annually until 5 years after transplantation, according to our institu- tional ... See full document
7
Repetitive DNA sequences as probes for Mycobacterium tuberculosis
... Since Southern blot hybridizations yield more specific information, the recombinants were hybridized with membranes containing BamHI digests ofDNA from members of the tuberculosis comple[r] ... See full document
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