[PDF] Top 20 Circularization of human immunodeficiency virus type 1 DNA in vitro.
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Circularization of human immunodeficiency virus type 1 DNA in vitro.
... Culture supernatant containing a high concentration of virus was Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Linear viral DNA present in cytoplasmic extracts of cel[r] ... See full document
11
Establishment of a stable, inducible form of human immunodeficiency virus type 1 DNA in quiescent CD4 lymphocytes in vitro.
... of virus production, at 7 days following each mitogen induction, that equaled the levels of virus produced during parallel infections of fully proliferating CD4 lymphocytes stimulated with mito- gen 3 days ... See full document
12
Evidence for Gene Expression by Unintegrated Human Immunodeficiency Virus Type 1 DNA Species
... viral DNA. However, the extent of viral DNA synthesis did not account for the dramatic decrease of viral RNA ...of virus-like particles in superna- tant ...HIV-1 DNA synthesis, ... See full document
9
DNA Damage Sensors ATM, ATR, DNA-PKcs, and PARP-1 Are Dispensable for Human Immunodeficiency Virus Type 1 Integration
... ficiency virus type 1 (HIV-1) and other ...completion. DNA damage sensors such as ATM (ataxia-telangiectasia mutated), ATR (ATM- and Rad3-related), DNA-PK (DNA-dependent ... See full document
6
In Vitro Resistance to the Human Immunodeficiency Virus Type 1 Maturation Inhibitor PA-457 (Bevirimat)
... conducted in this study enables us to anticipate the potential emergence of PA-457 resistance in vivo. This is significant, as PA-457 is currently undergoing clinical trials and the emer- gence of resistant isolates ... See full document
15
DNA substrate requirements for different activities of the human immunodeficiency virus type 1 integrase protein.
... Here we demonstrate that the hairpin loop formation is carried out only on a substrate containing viral DNA ends, which supports the notion that the intramolecular strand transfer reacti[r] ... See full document
8
Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells.
... Namalwa-CD4 cells are derivatives of a human B-cell line stably transfected with a CD4-expressing plasmid; they express high levels of cell surface CD4, are permissive for HIV-1 replicat[r] ... See full document
11
Human immunodeficiency virus type 1 evolution in vivo tracked by DNA heteroduplex mobility assays.
... DNA heteroduplex mobility and tracking analyses were used to monitor the generation of HIV sequence diversity, to estimate quasispecies complexity, and to assess the turnover of genetic [r] ... See full document
12
Human immunodeficiency virus type 1 Vpr protein binds to the uracil DNA glycosylase DNA repair enzyme.
... pressed protein lacking the N-terminal 84 amino acids. The mature nuclear form of UNG begins at residue 77, whereas UNG D 84 starts at residue 85. UNG D 84 corresponds in fact to the nuclear form of UNG deleted of eight ... See full document
8
DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 nucleocapsid protein.
... 9 DNA Strand Exchange and Selective DNA Annealing Promoted by the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein ZENTA TSUCHIHASHIt AND PATRICK 0.. BROWN* Howard Hughes Medical[r] ... See full document
8
In Vitro Analysis of Human Immunodeficiency Virus Type 1 Minus-Strand Strong-Stop DNA Synthesis and Genomic RNA Processing
... RNA- DNA heteroduplex and then reassociate, potentially undergo- ing multiple rounds of DNA synthesis and/or RNA cleavage on either the same or different ...of DNA synthesis in the presence of ... See full document
15
Conservation of an intact human immunodeficiency virus type 1 vif gene in vitro and in vivo.
... PCR. DNA present in patient 9 (Table 1) PBMC lysates was amplified by nested PCR, generating the ...proviral DNA from KS242 ...plasmid DNA was column purified (Qiagen plasmid kit; Qiagen, ... See full document
8
Detection of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein p7 In Vitro and In Vivo
... In summary, we developed an ECL-based p7 immunoassay with a dynamic range of 50 to 20,000 pg of p7 per ml. It was well suited to compete with p24 assays to monitor standard viral infections, independent of which ... See full document
5
A Mutation in Human Immunodeficiency Virus Type 1 Protease, N88S, That Causes In Vitro Hypersensitivity to Amprenavir
... (bold type): K20T (5⬘-GGGGGGCAATTAA C GGAAGCTCTATTAG-3⬘; cod- ing strand), M36I (5⬘-GTATTAGAAGAAAT A AATTTGCC AGGAAG-3⬘; coding strand), M46L (5⬘-GATGGAAACCAA AATTGATAGGGGGAATTG-3⬘; coding strand), L63P (5⬘-G ... See full document
6
Human Mast Cell Progenitors Can Be Infected by Macrophagetropic Human Immunodeficiency Virus Type 1 and Retain Virus with Maturation In Vitro
... to 1% of the cells infected with the HIV-1 YU2 single-round virus showed green fluorescence (as depicted in panel ...similar virus carrying the envelope glycoprotein of HIV-1 HXBc2 ... See full document
7
The DNA Deaminase Activity of Human APOBEC3G Is Required for Ty1, MusD, and Human Immunodeficiency Virus Type 1 Restriction
... a 1:1 ratio of A3G to MusD, wild-type A3G and the N- and C-terminal zinc-binding domain mutants caused significant decreases in MusD transposition (89%, 73%, and 51%, ...(wild type [WT]), ... See full document
9
Simian Immunodeficiency Virus Integration Preference Is Similar to That of Human Immunodeficiency Virus Type 1
... target DNA in a concerted cleavage and ligation ...that human immunodefi- ciency virus type 1 (HIV-1) greatly prefers integrating into transcription units within the host genome ... See full document
6
In vitro antigen challenge of human antibody libraries for vaccine evaluation: the human immunodeficiency virus type 1 envelope.
... on human antibodies react- ing with the vaccine; murine antibodies commonly available may reflect peculiarities of immunization in the animal ...in vitro by selecting the library against a potential ... See full document
5
Organization of Immature Human Immunodeficiency Virus Type 1
... (Fig. 1) forms spherical particles under in vitro assembly conditions ...in vitro-assembled par- ticles were concentrated by brief centrifugation and studied by ...in vitro-assembled particles ... See full document
13
Relative Replicative Fitness of Zidovudine-Resistant Human Immunodeficiency Virus Type 1 Isolates In Vitro
... wild type obtained ...“wild-type” virus may not be the variant which arises upon the removal of drug selection pressure (6, ...wild type would be expected to be present only at extremely low ... See full document
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