[PDF] Top 20 Comparison of Conventional PCR, Multiplex PCR, and Loop Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species
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Comparison of Conventional PCR, Multiplex PCR, and Loop Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species
... rcobacter species are Gram-negative, non-spore-forming, mo- tile, spiral-shaped bacteria that require a low-oxygen environ- ment and usually blood-based or complex media for their initial culture ...(1). ... See full document
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Comparison of Loop Mediated Isothermal Amplification and Real Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices
... sensitive conventional techniques for the detection of Strongyloides larvae in stool are dependent on live larvae and include agar plate culture, Harada culture, and the Baermann technique ...Real-time ... See full document
9
Rapid and Sensitive Detection of H7N9 Avian Influenza Virus by Use of Reverse Transcription–Loop Mediated Isothermal Amplification
... lar detection assays specific for this virus would be in great ...transcription–loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of the ... See full document
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Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens
... Eimeria species, but found incomplete species-specificity to be an occasional problem when working with archived samples (data not ...Eimeria species-specific qPCR panel reduced the sensitivity of ... See full document
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Three Isothermal Amplification Techniques for Rapid Identification of Cladophialophora carrionii, an Agent of Human Chromoblastomycosis
... are PCR based, but during the past 2 decades, several isothermal amplification tech- niques, which amplify DNA by strand replacement without using a thermocycler, have become ...developed ... See full document
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Loop Mediated Isothermal Amplification Method for Differentiation and Rapid Detection of Taenia Species
... Rapid detection and differentiation of Taenia species are required for the control and prevention of taeniasis and cysticercosis in areas where these diseases are ...the conventional diagnosis ... See full document
7
Comparison of a loop mediated isothermal amplification for orf virus withquantitative real time PCR
... a rapid, simple and sensitive detection method for ORFV infections is ...of conventional PCR or nested PCR for detecting ORFV infections ... See full document
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Combination of PURE DNA extraction and LAMP DNA amplification methods for accurate malaria diagnosis on dried blood spots
... several assays based on the LAMP ...Pan/Pf Detection Kit (Eiken Chemi- cal, Tokyo, Japan) is the first LAMP kit for the detection of malaria parasites to be commercialized in field-friendly packaging ... See full document
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Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification assays for Rapid Detection of Human Enterovirus 71 and Coxsackievirus A16 in Clinical Samples
... In comparison with the results ob- tained from commercial qRT-PCR diagnostic kits (DaAn Gene, China), the results from RT-PCR-kit assay showed a ...CVA16 detection was slightly less sensitive ... See full document
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Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... immunosorbent assays (ELISAs) [6]. However, these assays may be limited by false-negative results in early or chronic cases of infection ...real-time PCR (qPCR) has also been developed to detect and ... See full document
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Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.
... The present study reveals that the investi- gated water supplies, which are used for agriculture and camping and some time used by animals, are contaminated by Toxoplasma and Acanthamoeba species. Zarjoob River ... See full document
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<p>Loop-mediated isothermal amplification for detection of <em>Legionella pneumophila</em> in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran</p>
... This study had some limitations. The actual prevalence of Legionnaires’ disease cases may be higher than we reported, as we were investigating only L. pneumophila using PCR and LAMP due to its high environmental ... See full document
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Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... step. Detection process was performed in 405nm (ELISA reader Fax Stat 2100, USA) by surveying the change of color in the reaction mix due to addition of ABTS substrate using ELISA ...Roche PCR ELISA (DIG ... See full document
6
Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis
... nested PCR and the sensitivity ...nested PCR compared with the results of microscopy were equally high, we can propose that LAMP is as reliable as nested PCR for the clinical detection of four ... See full document
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Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus
... nested PCR assays requires knowledge of the gene sequences, careful primer de- sign, and assay ...high amplification efficiency of LAMP. How- ever, the product detection system with ethidium ... See full document
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Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
... molecular detection. 3) RT-LAMP is simple and easy to perform and only requires a temperature-controlled water bath, making it suitable for primary clinical settings or field use. Therefore, we believe that this ... See full document
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Rapid detection of the common avian leukosis virus subgroups by real time loop mediated isothermal amplification
... by PCR and virus isolation ...assay, conventional PCR, and virus ...and PCR, showed positive results detected by ELISA again (data not ... See full document
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Rapid and Specific Detection of Toxigenic Staphylococcus aureus: Use of Two Multiplex PCR Enzyme Immunoassays for Amplification and Hybridization of Staphylococcal Enterotoxin Genes, Exfoliative Toxin Genes, and Toxic Shock Syndrome Toxin 1 Gene
... the multiplex PCRs in a generic DNA EIA (GEN-ETI-K DEIA; Sorin, Saluggia, Italy) according to the manufacturer’s ...denatured PCR products at the optimal hybridization temperature (55°C for all used probes) ... See full document
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Toxin Genes and Other Characteristics ofStaphylococcus aureus Isolates from Milk of Cows with Mastitis
... by conventional methods and by PCR ...to species-specific parts of the gene encoding the 23S rRNA and the genes encoding coagulase, clumping factor, and protein ...Comparable PCR-based systems ... See full document
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Sensitive and Specific Detection of Staphylococcal Epidermolysins A and B in Broth Cultures by Flow Cytometry Assisted Multiplex Immunoassay
... Assay. After the wells of a porous, flat-bottom microtiter plate were wet with PBST coupled to a vacuum manifold, 2,500 coupled beads were placed in each well and then washed three times by vacuum filtration with 200 l ... See full document
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