[PDF] Top 20 Comparison of 5' and 3' long terminal repeat promoter function in human immunodeficiency virus.

... 3830-3840 0022-538X/94/$04.00+0 Copyright C 1994, American Society for Microbiology Comparison of 5' and 3' Long Terminal Repeat Promoter Function in Human Immunodeficiency Virus BEP KLA[r] ... See full document

... heterologous promoter in CV1 cells, a monkey kidney cell ...further 3' half site and 5 heptanucleotide repeats (Figure ...Furthermore 5 nuclear orphan receptors with unknown ligands, ... See full document

... Pt 3, Pt 6, Pt 7, Pt 10, and the positive control HIVHXB LTR (P ...Pt 5 exhibited marginal levels of transcription that were almost 100-fold less than that of the positive control HIVHXB LTR ...Pt ... See full document

... a human binding site for b-HLH proteins, contained in a human origin of DNA replication (6, 26), acts as a downregulator of transcription when cloned upstream of the enhancer region of the LTR, therefore ... See full document

... HIV 5′-LTR in resting CD4+ T cells ...that 5′-LTR methylation appears early after ART start in at least two different patterns that can be associated with the baseline clinical characteristics of each ... See full document

... alter promoter occupancy by using chromatin immu- noprecipitation (ChIP) assays (11, ...␤-actin promoter, an additional input control, was little af- fected by the global effects of TSA and showed changes ... See full document

... the function of this small transactivator ...HIV-1 promoter-enhancer motifs and the TAR element that constitutes the Tat binding site, we analyzed the sequence of the LTR region of each of these revertant ... See full document

... negative-sense promoter was approximately 10 times less than from the positive-sense pro- moter and was negatively regulated by ...this promoter is upregulated in response to PMA ...the 3 9 (relative ... See full document

... in human macrophages reduces HIV ...in human macrophages (16). To confirm the function of this reagent in primary human macrophages, nu- clear and cytosolic extracts were prepared from ... See full document

... the promoter is blocked by an HCMV gene product or an induced cellular ...Tat function by binding directly either to Tat or to some other protein necessary for Tat ... See full document

... mutant virus stocks of sufficient titer for infection and replication assays, an FIV provirus driven by a chimeric 5⬘ LTR encoding the SV40 early promoter en- hancer and TATA sequences was ...the ... See full document

... dual function as mRNA template for translation of Gag precursor protein and Gag-Pol polyprotein and as genomic RNA that is pack- aged into progeny virions ...the 5⬘ untranslated region (UTR) that are ... See full document

... A Comparison of COUP-TF binding in HeLa nuclear extracts to COUP and HIV-1 variant COUP oligonucleotide probes by gel mobility shift analysis.. HeLa nuclear extracts N.E.[r] ... See full document

... sequence 5⬘-CCAT-3⬘, which is recognized by the YY1 transcription ...the 5⬘ CCAT motif. This binding is necessary for RSV promoter function in vitro and in vivo, although YY1 is not by ... See full document

... Fig. 3 showed that 2-LTR circles can decline in abundance during a single-cycle infection, but in the example studied this was due mainly to the loss of infected ...stomatitis virus type G ... See full document

... For HIV, the first 18 nucleotides of the 3' terminus of the tRNALYS, including the CCA sequences added posttranscriptionally, base pair with the viral RNA 24 and serve as the primer for [r] ... See full document

... About five times more reverse transcriptase activity was recovered from Jurkat cells that had been transfected with the provirus deleted for the NRE sequence 1,372,900 more virus was rel[r] ... See full document

... DNase I protection with this partially purified HeLa extract revealed that mutations of both SPi-binding sites I and II resulted in slightly decreased protection over the enhancer, TATA,[r] ... See full document

... proviral long terminal repeat ...using human colon epithelial cell lines, demonstrate that the downstream elements are transactivated by expression of the c-fos protooncogene and can transmit ... See full document

... Chicken ovalbumin upstream region promoter transcription factor binds to a negative regulatory region in the human immunodeficiency virus type I long terminal repeat.. Regulation of HIV-[r] ... See full document