[PDF] Top 20 Complete sequence and genomic analysis of murine gammaherpesvirus 68.

... Murine gammaherpesvirus 68 ( g HV68) infects mice, thus providing a tractable small-animal model for analysis of the acute and chronic pathogenesis of ...molecular analysis of g HV68 ... See full document

... Murine gammaherpesvirus 68 (MHV-68) is a pathogen of murid rodents and was first isolated from Clethrionomys glareo- lus (bank vole) in Slovakia (3, 4, ...a gammaherpesvirus on the ... See full document

... MHV-68 genomic DNA, including BglII and EcoRI restriction sites in the respective upstream and downstream ...coding sequence as an EcoRI-XhoI fragment (see paragraph on plasmids, above) and ligated ... See full document

... MHV-68 genomic-DNA fragments indicated were individually cloned into pGL3-Luc-linker downstream of the firefly luciferase coding sequence. The plasmids were transfected into 293TRex-ZAP cells, ... See full document

... superficial analysis of the RNA species present indicates that the very highly expressed vtRNAs are the most abundant viral RNA within the ...of genomic RNA packaging by RNA ...the sequence level as ... See full document

... by murine gammaherpesvirus 68 (MHV-68) is an excellent small-animal model of gam- maherpesvirus pathogenesis in a natural ...rus, murine herpesvirus 76 (MHV-76), which was isolated at ... See full document

... Sequence analysis of the murine gammaherpesvirus 68 ( ␥ HV68) genome revealed an open reading frame (gene 4) which is homologous to a family of proteins known as the regulators of ... See full document

... acid sequence homology to EBV and HHV-8 Rta, it is likely that the N terminus of MHV-68 Rta contains DNA binding and dimerization domains while the activation domain is located at the C ...epitope ... See full document

... the complete immune system, B-cell- dependent immunoselection may drive the appearance of a form of latency from which gHV68 reactivates inefficiently under the ex vivo culture ...mice. Analysis of this ... See full document

... includes murine gammaherpesvirus 68 (MHV-68) and simian herpesvirus saimiri ...on genomic organi- zation as well as the ability to establish latency in lymphoid cells ...to ... See full document

... and analysis by quantitative real-time ...56°C. Genomic DNAs, including viral DNAs, were isolated by a standard method of phenol chloroform extraction and ethanol ...whole genomic DNA (50 ng) was ... See full document

... BamHI-C genomic fragment was subcloned into the modified pACYC177 plasmid, digested with ScaI, which cuts at genomic coordinate 45480, and treated with shrimp alkaline phosphatase (Roche ... See full document

... parental genomic subclone for the targeting plasmids was constructed by inser- tion of the 3,723-bp BamHI-BsrGI fragment of ␥HV68 (bp 101654 to 105377; WUMS sequence [67]), containing gene 72 and flanking ... See full document

... enzyme analysis led to the identification of a bacte- rial clone containing a BAC plasmid with the full-length MHV-68 genome (pHA3) ...vector sequence released a ...the complete genome of ... See full document

... FACS analysis, flow cytometry Protein G antibody binding beads (Polysciences Europe GmbH, Eppelheim, Germany) were coated with polyclonal rabbit anti-MHV- 68 antibody for 30 min at room ... See full document

... in genomic orientation sub- stantially enhanced mLANA expression ...unique sequence of the MHV68 genome (which was not present in the constructs used here) has enhancer activity for the promoter in the ... See full document

... PFU of the indicated viruses. (A) Lytic lung titer. At day 6 after infection, lungs were harvested, and virus titers were determined from organ homogenates by plaque assay on BHK-21 cells. Each symbol represents an ... See full document

... Murine gammaherpesvirus 68 (MHV-68) is a naturally oc- curring rodent pathogen (1) which, from the structure of the viral genome and limited sequence data, appears to be closely related ... See full document

... unmutated genomic fragment spanning the mutated ...unmutated genomic fragments used for cotransfections represented as light and dark gray ...mutated genomic fragment in the genomes of the ... See full document

... Southern analysis combined with the use of DpnI restric- tion enzyme that only ⬃5% of the total amount of the reporter represented the replicated product (data not ...orilyt sequence is to sup- port DNA ... See full document