[PDF] Top 20 Construction and characterization of an infectious cDNA clone of coxsackievirus A 10

... Total RNAs were extracted using MiniBEST Universal RNA Extraction Kit (Takara, Osaka, Japan) from the same weight of tissue homogenates (brain, intestine, limb skeletal muscles, heart, liver, and lung), respect- ively, ... See full document

... full-length cDNA clone divided into two large overlapping frag- ments amplified by ...approximately 10 3 virus particles were present per cell, which is two to four orders magnitude lower than that ... See full document

... viruses, cDNA clones of the entire viral genome can serve as a template for the generation of infectious ...These infectious cDNA clones pro- vide a platform for the manipulation of viral ... See full document

... with 10% ferritin-supplemented bovine calf serum (HyClone Laboratories, Logan, UT), 100 U/ml penicillin-streptomycin (Mediatech, Manassas, VA), and 1 ␮ g/ml amphotericin B (Sigma-Aldrich, ...pyruvate, 10% ... See full document

... Sequence analysis also showed that the env gene of WZS-PERV(2) was genetically similar to that of other human-tropic PERV infectious clones. With the env gene of PERV-WZSP [GenBank NO: EF133960] as refer- ence, G ... See full document

... full-length cDNA clone, the replicon was fully stable during its propagation in bacteria for more than 200 generations, as determined by restriction endonuclease ...the construction of the SARS-CoV ... See full document

... blue, 10% glycerol) at 97°C for 4 min, and proteins were electrophoresed on an SDS–10% polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane using a semidry electroblotting apparatus ... See full document

... Phylogenetic analyses have provided supporting evidence that multiple episodes of interspecies virus transmission have occurred between nonhuman primates and humans (9, 16). Examples are human T-lymphotropic viruses ... See full document

... molecular clone of PEDV strain PC22A. The infectious-clone-derived PEDV (icPEDV) replicated as efficiently as the parental virus in cell culture and in pigs, resulting in lethal disease in ... See full document

... taining 10% fetal calf serum, and CaCo-2 cells were propa- gated in RPMI 1640 containing 10% fetal calf ...or 10% (CaCo-2) serum at 37 8 C overnight, and cells expressing astroviral structural ... See full document

... recent construction of cDNAs encoding infectious RNAs for trans- missible gastroenteritis coronavirus (TGEV) (1, 21), human coronavirus strain 229E (18), and infectious bronchitis virus (3) will ... See full document

... the cDNA of the 5th passage of LY0801 strain were amplified for construction of DNA- launched infectious ...fragments clone 1 containing the hammerhead ribozyme and 1 to 3042 of LY0801 ... See full document

... R etroviral elements, termed endogenous retroviruses (ERVs), occupy about 8 to 10% of mammalian genomes (1). Most ERVs are defective due to genomic mutations and deletions, or their expression is epigenetically ... See full document

... full-length cDNA clones of flaviviruses. (i) Full-length cDNA clone can be assembled through in vitro ligation of cDNA ...Genome-length cDNA containing an upstream promoter for ... See full document

... The inoculation of grapevine plants with GALV has never been reported [1,6]. Having established that the GALV-Nf cDNA clone induces systemic infection in N. benthamiana plants, we tested the same construct ... See full document

... tional) using a Hybri-slot manifold (Life Technologies). RNA from cells trans- fected with plasmid pSGI was used as a negative control. In addition, the in vitro-transcribed, unlabeled sense and antisense HEV RNAs (2.5 ... See full document

... Phenotypes of mutants used in this study Plaque phenotype Description of phenotype 2A-1 Minute plaque At all temperatures, mutant plaques clearly visible only 1 day after all 2B-201 Smal[r] ... See full document

... A total of 708 sequences (including six complete VP1 genome sequences determined by our laboratory) with complete VP1 region of CV-A16 were included in the phylogenetic analysis. Samples collected from 14 discrete ... See full document

... full-length cDNA copies of the viral ...full-length cDNA was constructed by ligating the overlapping fragments at convenient restriction sites into pBST KS 2 (Strat- agene) or pBKCMV (Stratagene) ... See full document

... of infectious virus would be an invaluable tool for various in-depth investigations on these ...the construction of DEV infectious clones, and based on the established platform, exogenous genes such ... See full document