[PDF] Top 20 Exon 3 of the Human Cytomegalovirus Major Immediate-Early Region Is Required for Efficient Viral Gene Expression and for Cellular Cyclin Modulation
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Exon 3 of the Human Cytomegalovirus Major Immediate-Early Region Is Required for Efficient Viral Gene Expression and for Cellular Cyclin Modulation
... 1:15,000; major capsid protein (MCP) MAb 28-4, 1:20; pp28 MAb, 1:10,000;  -actin MAb AC-15, 1:10,000; cyclin B1, 1:500; cyclin A MAb CYA06, 1:200; cyclin E sc-198, ... See full document
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Inhibition of Human Cytomegalovirus Immediate-Early Gene Expression by Cyclin A2-Dependent Kinase Activity
... the expression of immedi- ate-early (IE) ...many cellular functions, including apoptosis (18, 43, 44, 62), cellular DNA synthesis (48, 72), STAT signaling (51), protein kinase R activity (10, ... See full document
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A Strong Negative Transcriptional Regulatory Region between the Human Cytomegalovirus UL127 Gene and the Major Immediate-Early Enhancer
... The human CMV MIE enhancer and the flanking regions were assigned arbitrary boundaries to better discuss this com- plicated regulatory region (52, ...control region from ⫹8 to ⫹112 referred to as ... See full document
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Alternative Splicing of the Human Cytomegalovirus Major Immediate-Early Genes Affects Infectious-Virus Replication and Control of Cellular Cyclin-Dependent Kinase
... in exon 4 was first reported by Shirakata et ...under immediate-early ...infected human monocyte- derived macrophages or cycloheximide-treated HFF cells (4, ...two gene products, 60 kDa ... See full document
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Human Cytomegalovirus Early Protein pUL21a Promotes Efficient Viral DNA Synthesis and the Late Accumulation of Immediate-Early Transcripts
... the early events of viral infection, including reduced MIE gene expression and viral DNA replication, thus compli- cating the analysis defining the precise role of this IE autore- ... See full document
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Requirement of Multiple cis-Acting Elements in the Human Cytomegalovirus Major Immediate-Early Distal Enhancer for Viral Gene Expression and Replication
... The human cytomegalovirus (HCMV) major immediate- early (MIE) gene products, IE1 p72 and IE2 p86, are required for initiating viral replication (10, 16, 21, ... See full document
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Interaction between the Human Cytomegalovirus UL82 Gene Product (pp71) and hDaxx Regulates Immediate-Early Gene Expression and Viral Replication
... regulates efficient HCMV rep- ...the cellular pro- tein hDaxx during HCMV infection (15, ...of gene expression (9, 28, 29, 32, 35, ...active gene transcription and viral genome ... See full document
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The Human Cytomegalovirus Major Immediate-Early Enhancer Determines the Efficiency of Immediate-Early Gene Transcription and Viral Replication in Permissive Cells at Low Multiplicity of Infection
... The human CMV enhancer in the context of the viral genome is unidirectional for the expression of the IE1 and IE2 ...the expression of either ie1 and ie3 or ie2 (Fig. 1A). The human CMV ... See full document
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RNA Interference-Mediated Targeting of Human Cytomegalovirus Immediate-Early or Early Gene Products Inhibits Viral Replication with Differential Effects on Cellular Functions
... with human cytomegalovirus ...shared exon 3 of UL123 and UL122 transcripts encoding IE1 and IE2 immediate-early proteins of ...of viral protein expression, DNA ... See full document
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Roles of Polypyrimidine Tract Binding Proteins in Major Immediate-Early Gene Expression and Viral Replication of Human Cytomegalovirus
... MIE gene products that are all at the protein pro- duction ...MIE gene splicing. MIE gene splicing should be enhanced when the PTB gene is knocked down, which would result not only in higher ... See full document
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The Human Cytomegalovirus Major Immediate-Early Distal Enhancer Region Is Required for Efficient Viral Replication and Immediate-Early Gene Expression
... enhancer region is needed for regulation of the MIE genes during a productive viral infection is ...LS gene expression during viral latency is unknown, although deletion of this ... See full document
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Inactivating a Cellular Intrinsic Immune Defense Mediated by Daxx Is the Mechanism through Which the Human Cytomegalovirus pp71 Protein Stimulates Viral Immediate-Early Gene Expression
... Near complete Daxx degradation is observed at low MOIs because of the high particle/PFU ratio of the crude viral stocks used. Indeed, we have shown that almost half of the cells in a population stain positive for ... See full document
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Structural organization, expression, and functional characterization of the murine cytomegalovirus immediate-early gene 3.
... The promoter of this HCMV early gene can be activated by Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In this communication, we report on i the structure of the ie3 [r] ... See full document
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Organization and expression of the immediate early genes of human cytomegalovirus.
... Since immediate early gene expression of human cytomegalovirus is dominated by the synthesis of an mRNA from the region of 0.739 to 0.751 map units that codes for the predominant immedia[r] ... See full document
14
The US16 Gene of Human Cytomegalovirus Is Required for Efficient Viral Infection of Endothelial and Epithelial Cells
... affect viral replication in endothelial and epithelial cells has been sug- gested by the results of whole-genome functional profiling of a bacterial artificial chromosome (BAC)-based clone derived from the Towne ... See full document
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Dysregulation of Cyclin E Gene Expression in Human Cytomegalovirus-Infected Cells Requires Viral Early Gene Expression and Is Associated with Changes in the Rb-Related Protein p130
... endogenous cyclin E—require- ment for early gene ...the cyclin E promoter and a luciferase reporter suggested that HCMV- mediated upregulation of cyclin E was E2F site independent ... See full document
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The Cellular Protein SPT6 Is Required for Efficient Replication of Human Cytomegalovirus
... or expression vectors in the heterokaryon ...and expression plasmid was sequenced to confirm the specific point mutation and rule out the possibility of a secondary mutation elsewhere in the ...on ... See full document
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Repression of HMGA2 Gene Expression by Human Cytomegalovirus Involves the IE2 86-Kilodalton Protein and Is Necessary for Efficient Viral Replication and Inhibition of Cyclin A Transcription
... RNA expression is that the domain between aa 136 and 290 is involved in the regulation of ...the region between aa 136 and 290 was one of three domains that were able to independently mediate these inter- ... See full document
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Variations in the cytomegalovirus major immediate early gene found by direct genomic sequencing
... An assay to detect and sequence DNA from human cytomegalovirus HCMV immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction [r] ... See full document
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Requirements for Enhanced Transgene Expression by Untranslated Sequences from the Human Cytomegalovirus Immediate-Early Gene
... To in- crease gene expression following nonviral deliv- ery of DNA, we investigated whether addition of untranslated sequences from the CMV IE gene would increase transgene expression in[r] ... See full document
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