[PDF] Top 20 Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread.
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Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread.
... To further investigate the roles of the central and flanking segments in 2a, we have constructed a series of deletion and frameshift mutants in a biologically active BMV RNA2 cDNA clone [r] ... See full document
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RNA Recombination in Brome Mosaic Virus: Effects of Strand-Specific Stem-Loop Inserts
... total RNA was isolated from separate local lesions as described previously (4, 31, 40) and the progeny RNA3 sequences were amplified by reverse transcription-PCR (RT-PCR) ...BMV RNA recombination constructs ... See full document
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A Brome Mosaic Virus Intergenic RNA3 Replication Signal Functions with Viral Replication Protein 1a To Dramatically Stabilize RNA In Vivo
... efficient RNA replication and subgenomic RNA synthesis ...1a, 2a, and RNA3 templates demonstrated that sequences within the intergenic region of RNA3 stimulate negative-strand synthesis in ... See full document
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Packaging of Brome Mosaic Virus Subgenomic RNA Is Functionally Coupled to Replication-Dependent Transcription and Translation of Coat Protein
... viral replication resulting in the formation of active replicase functions as a specificity filter by enhancing packaging of viral RNA but not ubiquitous cellular RNA or nonreplicating viral ... See full document
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Brome Mosaic Virus Protein 1a Recruits Viral RNA2 to RNA Replication through a 5′ Proximal RNA2 Signal
... first deletion derivative tested, B2⌬5⬘-1, lacked nt 1 to 46 of ...wt 2a codons followed by 18 out-of-frame ...wt 2a codons followed by 3 out-of-frame ...total RNA prepared from yeast ... See full document
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Brome Mosaic Virus Polymerase-Like Protein 2a Is Directed to the Endoplasmic Reticulum by Helicase-Like Viral Protein 1a
... Brome mosaic virus (BMV), a positive-strand RNA virus in the alphavirus-like superfamily, encodes RNA replication proteins 1a and ...viral RNA capping, and ... See full document
9
Brome Mosaic Virus RNA Syntheses In Vitro and in Barley Protoplasts
... Cucumber mosaic virus (CMV) SLC differs from the one in BMV in that a highly conserved dinucleotide in the loop, 5⬘CA3⬘, replaces the CAM ...insertion, deletion, and point mutations shown in ... See full document
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The polymerase-like core of brome mosaic virus 2a protein, lacking a region interacting with viral 1a protein in vitro, maintains activity and 1a selectivity in RNA replication.
... while 2a contains a polymerase (pol)-like core flanked by N- and C-terminal ...BMV RNA replication requires 1a-2a compatibility, implying direct or indirect 1a-2a interaction in ...of ... See full document
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cis- and trans-Acting Functions of Brome Mosaic Virus Protein 1a in Genomic RNA1 Replication
... RNA1 replication could result from a need for the act of translation, perhaps to reconfigure the structure of ...(Fig. 2A). Both mutants can effi- ciently translate the truncated protein in rabbit ... See full document
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A mathematical model of RNA3 recruitment in the replication cycle of Brome mosaic virus
... (+)RNA virus biology is the replication of the plant brome mosaic virus (BMV) ...viral RNA replication factors protein 1a and 2a respectively ... See full document
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Host-specific alterations in viral RNA accumulation and infection spread in a brome mosaic virus isolate with an expanded host range.
... of virus-host interaction and the determinants of viral host range, we constructed full-length cDNA clones to all three genomic RNAs of an unusual brome mosaic virus (BMV) isolate with an ... See full document
8
Host Deadenylation-Dependent mRNA Decapping Factors Are Required for a Key Step in Brome Mosaic Virus RNA Replication
... BMV RNA templates to the replication complex requires expression of the BMV 1a protein but occurs in the absence of BMV 2a polymerase ...on RNA recruit- ment, it was important to obtain ... See full document
6
A Novel Methyltransferase Methylates Cucumber Mosaic Virus 1a Protein and Promotes Systemic Spread
... Cucumber mosaic virus (CMV) is a type member of the genus Cucumovirus in the family ...positive-sense RNA genome with components designated RNAs 1 to 3 ...a protein involved in the replica- ... See full document
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Deletion of the C-terminal 33 amino acids of cucumber mosaic virus movement protein enables a chimeric brome mosaic virus to move from cell to cell.
... Four-base deletion in CMV. The 4-base deletion was intro- duced into the MP gene in C3 to create the CMV RNA 3 mutant (C3-D4) ...of spread of the infection site for the mutant was lower than ... See full document
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Mutation of Host Δ9 Fatty Acid Desaturase Inhibits Brome Mosaic Virus RNA Replication between Template Recognition and RNA Synthesis
... positive-strand RNA viruses assemble their RNA replication complexes on intracellular ...membranes. Brome mosaic virus (BMV) replicates its RNA in endoplasmic reticulum ... See full document
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Replicase-Binding Sites on Plus- and Minus-Strand Brome Mosaic Virus RNAs and Their Roles in RNA Replication in Plant Cells
... both RNA strands and in the inter- cistronic region of RNA3 ...BMV RNA accumulation in transfected ...a deletion of the polyade- nylate tract resulted in debilitated RNA ...directing ... See full document
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Mutations in the Capsid Protein of Brome Mosaic Virus Affecting Encapsidation Eliminate Vesicle Induction In Planta: Implications for Virus Cell-to-Cell Spread
... dissecting virus movement at the cellular level made in the last decade revealed that many viruses traffic through the cellular endomembrane sys- tem ...Potato virus X (PVX), one of the three proteins re- ... See full document
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Interaction between Brome Mosaic Virus Proteins and RNAs: Effects on RNA Replication, Protein Expression, and RNA Stability
... the effects of pCPKOII and MPKO on BMV RNA levels in barley protoplasts to allow comparisons to the effects observed in tobacco ...minus-strand RNA replication was ...the effects ... See full document
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An Alternate Pathway for Recruiting Template RNA to the Brome Mosaic Virus RNA Replication Complex
... multidomain RNA replication protein 1a of brome mosaic virus (BMV), a positive-strand RNA virus in the alphavirus-like superfamily, plays key roles in assembly and ... See full document
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RNA Synthesis by the Brome Mosaic Virus RNA-Dependent RNA Polymerase in Human Cells Reveals Requirements for De Novo Initiation and Protein-Protein Interaction
... enhance 2a activity, the membrane reconfiguration activity of 1a is not required to enhance 2a activ- FIG 4 BMV 2a residues required for polymerase activity and for interaction with ...BMV 2a ... See full document
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