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[PDF] Top 20 Detection of Chlamydia trachomatis by Isothermal Ramification Amplification Method: a Feasibility Study

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Detection of Chlamydia trachomatis by Isothermal Ramification Amplification Method: a Feasibility Study

Detection of Chlamydia trachomatis by Isothermal Ramification Amplification Method: a Feasibility Study

... Chlamydia trachomatis is the leading cause of sexually transmitted disease in the United ...and detection of C. trachomatis in a single collection ...this study we determined whether ... See full document

5

Prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and Ureaplasma urealyticum infections using a novel isothermal simultaneous RNA amplification testing method in infertile males

Prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and Ureaplasma urealyticum infections using a novel isothermal simultaneous RNA amplification testing method in infertile males

... multicentre study involved following medi- cal centers: the First Affiliated Hospital of Wenzhou Medical University, Changhai Hospital, the 324 Hospital of PLA while the data was summarized and analyzed in the ... See full document

7

Combination with antimicrobial peptide lyses improves loop mediated isothermal amplification based method for Chlamydia trachomatis detection directly in urine sample

Combination with antimicrobial peptide lyses improves loop mediated isothermal amplification based method for Chlamydia trachomatis detection directly in urine sample

... for Chlamydia trachomatis clinical diagnostics is widely used PCR-based techniques (Abbot Real Time CT/NG assay; Roche Cobas Amplicor CT/NG assay eg) which is highly specific (amplify the specific DNA ... See full document

8

Urine Specimens from Pregnant and Nonpregnant Women Inhibitory to Amplification of Chlamydia trachomatis Nucleic Acid by PCR, Ligase Chain Reaction, and Transcription Mediated  Amplification: Identification of Urinary Substances  Associated with Inhibitio

Urine Specimens from Pregnant and Nonpregnant Women Inhibitory to Amplification of Chlamydia trachomatis Nucleic Acid by PCR, Ligase Chain Reaction, and Transcription Mediated Amplification: Identification of Urinary Substances Associated with Inhibition and Removal of Inhibitory Activity

... internal amplification control to identify specimens containing PCR ...our study the internal control de- tected 13 inhibitory specimens, while our exogenous DNA spike method detected 19 inhibitory ... See full document

5

Evaluation of the NucliSens Basic Kit for Detection ofChlamydia trachomatis and Neisseria gonorrhoeaein Genital Tract  Specimens Using Nucleic Acid Sequence Based  Amplification of 16S rRNA

Evaluation of the NucliSens Basic Kit for Detection ofChlamydia trachomatis and Neisseria gonorrhoeaein Genital Tract Specimens Using Nucleic Acid Sequence Based Amplification of 16S rRNA

... an isothermal amplification at 41°C, the optimal annealing temperature for primers does not have to be deter- mined ...the detection level by using a specific capture probe for ...C. ... See full document

7

Comparison of Three Nucleic Acid Amplification Tests for Detection of Chlamydia trachomatis in Urine Specimens

Comparison of Three Nucleic Acid Amplification Tests for Detection of Chlamydia trachomatis in Urine Specimens

... The study reported here was conducted to compare the performance of three NAATs based on different technologies ...C. trachomatis in urine specimens from high-risk ...C. trachomatis detection ... See full document

5

Evaluation of High Resolution Typing Methods for Chlamydia trachomatis in Samples from Heterosexual Couples

Evaluation of High Resolution Typing Methods for Chlamydia trachomatis in Samples from Heterosexual Couples

... typing method greatly depends on the initial detection method that was used to diagnose the infec- ...this study, the samples were screened using a highly sensitive detection system for ... See full document

10

Performance of Transcription Mediated Amplification and Ligase Chain Reaction Assays for Detection of Chlamydial Infection in Urogenital Samples Obtained by Invasive and Noninvasive Methods

Performance of Transcription Mediated Amplification and Ligase Chain Reaction Assays for Detection of Chlamydial Infection in Urogenital Samples Obtained by Invasive and Noninvasive Methods

... Since chlamydial infection is a sexually transmitted disease, a high assay specificity is needed to exclude false-positive re- sults. In the present study, the numbers of unconfirmed and, therefore, false-positive ... See full document

5

Comparison of the PACE 2 Assay, Two Amplification Assays, and Clearview EIA for Detection of Chlamydia trachomatis  in Female Endocervical and Urine Specimens

Comparison of the PACE 2 Assay, Two Amplification Assays, and Clearview EIA for Detection of Chlamydia trachomatis in Female Endocervical and Urine Specimens

... this study was to compare the current test methods for detection of Chlamydia trachomatis used at the University of South Alabama, the PACE 2 assay (Gen-Probe) and the Clearview EIA (Wampole ... See full document

7

RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples

RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples

... ensure detection of all C. trachomatis-positive ...most amplification systems for the routine diagnosis of ...C. trachomatis variants has been reported (1, 2, ...C. trachomatis could ... See full document

7

Detection of Chlamydia trachomatis andNeisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic  Acid Amplification Tests

Detection of Chlamydia trachomatis andNeisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic Acid Amplification Tests

... DNA amplification assays can be ensured by retesting initially positive specimens by a different amplifi- cation method, as was done in the present ...reference method but positive by the test under ... See full document

6

Evaluation of Chlamydia trachomatis and Neisseria gonorrhoeae Detection in Urine, Endocervical, and Vaginal Specimens by a Multiplexed Isothermal Thermophilic Helicase Dependent Amplification (tHDA) Assay

Evaluation of Chlamydia trachomatis and Neisseria gonorrhoeae Detection in Urine, Endocervical, and Vaginal Specimens by a Multiplexed Isothermal Thermophilic Helicase Dependent Amplification (tHDA) Assay

... for amplification and real-time detection of PCR ...C. trachomatis or N. gonorrhoeae DNA. Detection of any amount of target in the qPCR was counted as a positive ... See full document

5

Tubal Infertility and Chlamydia Trachomatis in a Congolese Infertile Population

Tubal Infertility and Chlamydia Trachomatis in a Congolese Infertile Population

... current study, the CAT titer was positive for ...current study highlights the preponderant role of chlamydia trachomatis in our setting and the CAT usefulness in infertility ... See full document

10

Molecular techniques for the detection of Chlamydia trachomatis

Molecular techniques for the detection of Chlamydia trachomatis

... Upon cleansing the cervix, four swabs were taken and used for each of the four detection methods evaluated in this study i.e., culture, probe, DFA, and ELISA.. Cotton swabs American Scie[r] ... See full document

5

Detection of Four Plasmodium Species by Genus  and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis

Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis

... Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the clinical detection of four species of human malaria parasites: Plasmodium ... See full document

8

The role of zoonotic chlamydial agents in ruminants abortion

The role of zoonotic chlamydial agents in ruminants abortion

... recognize Chlamydia and Chlamydia in biological samples ...suspected Chlamydia abortion (last 2-3 weeks of pregnancy) in ruminants (bovine, ovine and goat) in the 2 mentioned prov- inces and ... See full document

7

Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis

Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis

... this study is based on the 18S rRNA gene sequence, and this target has been found to be highly efficient for the diagnosis of leishmaniasis from human clinical material ... See full document

6

Determination of Chlamydia trachomatisPrevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens

Determination of Chlamydia trachomatisPrevalence in an Asymptomatic Screening Population: Performances of the LCx and COBAS Amplicor Tests with Urine Specimens

... the amplification tubes containing 50 ␮l of am- plification ...each amplification reaction (in the amplification mixture) and is coamplified with the possible ...C. trachomatis target DNA from ... See full document

5

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Strand Displacement Amplification and Relevance of the Amplification Control for Use with Vaginal Swab Specimens

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Strand Displacement Amplification and Relevance of the Amplification Control for Use with Vaginal Swab Specimens

... this study and repeat testing to resolve the results for sam- ples with indeterminate results was $13,000 for supplies ...the detection of six additional positive specimens cost $2,166 in additional ... See full document

5

Female Epidemiology of Transcription Mediated Amplification Based Trichomonas vaginalis Detection in a Metropolitan Setting with a High Prevalence of Sexually Transmitted Infection

Female Epidemiology of Transcription Mediated Amplification Based Trichomonas vaginalis Detection in a Metropolitan Setting with a High Prevalence of Sexually Transmitted Infection

... C. trachomatis, and N. gonorrhoeae detection rates were reported by Ginocchio et ...because study site enroll- ment represented several geographical regions, a number of study centers were not ... See full document

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