[PDF] Top 20 Detection and Identification of Ehrlichia Species in Blood by Use of PCR and Electrospray Ionization Mass Spectrometry
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Detection and Identification of Ehrlichia Species in Blood by Use of PCR and Electrospray Ionization Mass Spectrometry
... The PCR/ESI-MS assay employs 16 primer pairs and is designed for use with the T5000 PCR mass spectrometry system (Ibis Biosciences, Carlsbad, ...(e.g., Ehrlichia, Anaplasma, Bar- ... See full document
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Use of PCR Coupled with Electrospray Ionization Mass Spectrometry for Rapid Identification of Bacterial and Yeast Bloodstream Pathogens from Blood Culture Bottles
... in blood culture ...the PCR/ESI-MS technology ...port, PCR/ESI-MS has the ability to evaluate samples for the antibiotic resistance genes contained in the genomes of partic- ular organisms of ... See full document
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Detection, Identification, and Distribution of Fungi in Bronchoalveolar Lavage Specimens by Use of Multilocus PCR Coupled with Electrospray Ionization/Mass Spectrometry
... pathogen species in a single ...the PCR/ESI-MS-based kit for detection and identification of fungi from BAL fluid ...multilocus PCR amplification and ESI-MS detection in order to ... See full document
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Broad Range Direct Detection and Identification of Fungi by Use of the PLEX ID PCR Electrospray Ionization Mass Spectrometry (ESI MS) System
... following PCR am- plification by manually loading the plate onto the PLEX-ID system where the PCR product was desalted and analyzed by using mass spectrometry (Abbott Molecular, Des Plaines, ... See full document
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Enhanced Diagnostic Yields of Bacteremia and Candidemia in Blood Specimens by PCR Electrospray Ionization Mass Spectrometry
... microbial detection method is the time-consuming process of microbiological ...microbial identification and antibiotic suscep- tibility determination require an additional 6 to 24 h ...10). Blood ... See full document
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Comparison of PCR Electrospray Ionization Mass Spectrometry with 16S rRNA PCR and Amplicon Sequencing for Detection of Bacteria in Excised Heart Valves
... preparation, PCR, desalting, ESI-MS analysis, data processing, and reporting within a 6-h period ...microbial identification is provided using a signature database after mathematical matching of observed ... See full document
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Simultaneous Identification of Mycobacterial Isolates to the Species Level and Determination of Tuberculosis Drug Resistance by PCR Followed by Electrospray Ionization Mass Spectrometry
... mycobacterial species from liquid cul- ture ...the PCR/ESI-MS identification and the reference identification is shown in Table ...by PCR/ESI-MS and could be further segregated into ... See full document
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PCR Electrospray Ionization Mass Spectrometry for Direct Detection of Pathogens and Antimicrobial Resistance from Heart Valves in Patients with Infective Endocarditis
... Unlike mass spectrometry adapted for identification of bac- teria and fungi isolated from cultures, PCR/ESI-MS, as a result of a PCR-amplification step, offers the possibility for ... See full document
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Identification of Mycobacterium Species and Mycobacterium tuberculosis Complex Resistance Determinants by Use of PCR Electrospray Ionization Mass Spectrometry
... Resistance to INH has a high correlation with mutations pres- ent within two genes: katG (encoding a catalase-peroxidase en- zyme required for isoniazid activation) and inhA (promoter re- gion). Mutations within the katG ... See full document
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Reverse Transcription PCR–Electrospray Ionization Mass Spectrometry for Rapid Detection of Biothreat and Common Respiratory Pathogens
... sheep blood (Remel, Lenexa, KS), chocolate agar (Remel), MacConkey agar (Remel), and standard and selective buffered charcoal yeast extract agar (BBL, Sparks, MD) for recovery of Legionella ... See full document
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Comparative Analysis of Two Broad Range PCR Assays for Pathogen Detection in Positive Blood Culture Bottles: PCR–High Resolution Melting Analysis versus PCR Mass Spectrometry
... Electrospray ionization mass ...automated mass spectrometry platform (Abbott Molecular). PCR products underwent an automated weak anion ex- change protocol for purification, and ... See full document
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PCR Followed by Electrospray Ionization Mass Spectrometry for Broad Range Identification of Fungal Pathogens
... The PCR/ESI-MS system used here lists for approximately $500,000, with costs of $30 per sample and a turnaround time of 24 ...Fungal identification by phenotypic methods costs $5 per ...systems. ... See full document
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Diagnosis of Prosthetic Joint Infection by Use of PCR Electrospray Ionization Mass Spectrometry
... of detection between 20 and 500 CFU/ml in blood ...and PCR-ESI/MS using three definitions of PJI are shown in Table ...for PCR-ESI/MS and culture, respectively (P ⫽ ...by PCR-ESI/MS and ... See full document
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Rapid Detection and Molecular Serotyping of Adenovirus by Use of PCR Followed by Electrospray Ionization Mass Spectrometry
... uses PCR coupled with electrospray ionization mass spec- trometry (PCR/ESI-MS) (11, 12, 17, ...broad-range PCR primers capable of producing unique amplicons from a wide variety ... See full document
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Broad Range PCR Coupled with Electrospray Ionization Time of Flight Mass Spectrometry for Detection of Bacteremia and Fungemia in Patients with Neutropenic Fever
... liquid blood culture (BC), followed by Gram stain, subculturing, identification, and antimicrobial susceptibility ...of blood cultures (range, 2 to 5 days) are major ...the detection of an ... See full document
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Broad Range PCR Electrospray Ionization Mass Spectrometry for Detection and Typing of Adenovirus and Other Opportunistic Viruses in Stem Cell Transplant Patients
... virus detection in plasma is associated with nephropathy in kidney transplant pa- tients, and it was recently shown that BK viremia preceded hem- orrhagic cystitis in children undergoing allogeneic HSCT ... See full document
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Metabolomics Investigation of Glyceollins by On-Line Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry and Fungal Metabolite Identification by Thermal Desorption Analysis Coupled with Gas Chromatography-Mass Spectrometry
... analyte species may enter the ESI source of the mass spectrometer free from ...tandem mass spectrometry can enable direct mixture analyses in the absence of chromatographic ...sample ... See full document
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Biotransformation of ginsenosides Rb1, Rg3 and Rh2 in rat gastrointestinal tracts
... nents. Mass spectrometry (MS) has progressed extremely rapidly during the last decade; especially in production, separation and ejection of ions, data acquisition and data ...the mass spectrometer ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... desorption ionization–time of flight (MALDI-TOF) mass spectrometry represents a revolution in the rapid iden- tification of bacterial and fungal pathogens in the clinical microbiology ...positive ... See full document
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Ranitidine hydrochloride: An update on analytical, clinical and pharmacological aspects
... the use of this drug may associated with some side effects which include headache, skin rashes, tiredness, constipation, nausea, diarrhoea, bradycardia, hypersensitivity, contact dermatitis, urticaria ... See full document
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